Titre :

MuscleJ2: a rebuilding of MuscleJ with new features for high-content analysis of skeletal muscle immunofluorescence slides

Auteur(s) :

Danckaert, Anne [Auteur correspondant]
Plateforme technologique Bioimagerie Photonique - Photonic BioImaging Technologic Platform
Trignol, Aurélie [Auteur]
Institut de Recherche Biomédicale des Armées [Brétigny-sur-Orge] [IRBA]
Le Loher, Guillaume [Auteur]
Plateforme technologique Bioimagerie Photonique - Photonic BioImaging Technologic Platform
Ecole Centrale d'Electronique
Loubens, Sébastien [Auteur]
Récepteurs Nucléaires, Maladies Métaboliques et Cardiovasculaires - U 1011 [RNMCD]
Centre Hospitalier Régional Universitaire [CHU Lille] [CHRU Lille]
Staels, Bart [Auteur]
Récepteurs Nucléaires, Maladies Métaboliques et Cardiovasculaires - U 1011 [RNMCD]
Duez, Hélène [Auteur]
Récepteurs Nucléaires, Maladies Métaboliques et Cardiovasculaires - U 1011 [RNMCD]
Shorte, Spencer [Auteur]
Plateforme technologique Bioimagerie Photonique - Photonic BioImaging Technologic Platform
Mayeuf-Louchart, Alicia [Auteur correspondant]
Récepteurs Nucléaires, Maladies Métaboliques et Cardiovasculaires - U 1011 [RNMCD]

Titre de la revue :

Skeletal Muscle

Pagination :

14

Éditeur :

BioMed Central

Date de publication :

2023-08-23

ISSN :

2044-5040

Mot(s)-clé(s) en anglais :

Histology
Muscle fber morphology
Centro- and perinuclei
Fiber typing
Vascularization
Phenotype cartography
Extracellular matrix
Interstitial cells
Sarcolemmal staining

Discipline(s) HAL :

Sciences du Vivant [q-bio]

Résumé en anglais : [en]

Histological analysis of skeletal muscle is of major interest for understanding its behavior in different pathophysiological conditions, such as the response to different environments or myopathies. In this context, many ...
Lire la suite >Histological analysis of skeletal muscle is of major interest for understanding its behavior in different pathophysiological conditions, such as the response to different environments or myopathies. In this context, many software programs have been developed to perform automated high-content analysis. We created MuscleJ, a macro that runs in ImageJ/Fiji on batches of images. MuscleJ is a multianalysis tool that initially allows the analysis of muscle fibers, capillaries, and satellite cells. Since its creation, it has been used in many studies, and we have further developed the software and added new features, which are presented in this article. We converted the macro into a Java-language plugin with an improved user interface. MuscleJ2 provides quantitative analysis of fibrosis, vascularization, and cell phenotype in whole muscle sections. It also performs analysis of the peri-myonuclei, the individual capillaries, and any staining in the muscle fibers, providing accurate quantification within regional sublocalizations of the fiber. A multicartography option allows users to visualize multiple results simultaneously. The plugin is freely available to the muscle science community.Lire moins >

Langue :

Anglais

Comité de lecture :

Oui

Audience :

Internationale

Vulgarisation :

Non

Projet ANR :

EGID Diabetes Pole
Développment d'une infrastructure française distribuée coordonnée

Collections :

• Récepteurs nucléaires, Maladies Cardiovasculaires et Diabète (RNMCD) - U1011

Source :

Harvested from HAL