Title :

MuscleJ2: a rebuilding of MuscleJ with new features for high-content analysis of skeletal muscle immunofluorescence slides

Author(s) :

Danckaert, Anne [Auteur correspondant]
Plateforme technologique Bioimagerie Photonique - Photonic BioImaging Technologic Platform
Trignol, Aurélie [Auteur]
Institut de Recherche Biomédicale des Armées [Brétigny-sur-Orge] [IRBA]
Le Loher, Guillaume [Auteur]
Ecole Centrale d'Electronique
Plateforme technologique Bioimagerie Photonique - Photonic BioImaging Technologic Platform
Loubens, Sébastien [Auteur]
Centre Hospitalier Régional Universitaire [CHU Lille] [CHRU Lille]
Récepteurs Nucléaires, Maladies Métaboliques et Cardiovasculaires - U 1011 [RNMCD]
Staels, Bart [Auteur]
Récepteurs Nucléaires, Maladies Métaboliques et Cardiovasculaires - U 1011 [RNMCD]
Duez, Hélène [Auteur]
Récepteurs Nucléaires, Maladies Métaboliques et Cardiovasculaires - U 1011 [RNMCD]
Shorte, Spencer [Auteur]
Plateforme technologique Bioimagerie Photonique - Photonic BioImaging Technologic Platform
Mayeuf-Louchart, Alicia [Auteur correspondant]
Récepteurs Nucléaires, Maladies Métaboliques et Cardiovasculaires - U 1011 [RNMCD]

Journal title :

Skeletal Muscle

Pages :

14

Publisher :

BioMed Central

Publication date :

2023-08-23

ISSN :

2044-5040

English keyword(s) :

Histology
Muscle fber morphology
Centro- and perinuclei
Fiber typing
Vascularization
Phenotype cartography
Extracellular matrix
Interstitial cells
Sarcolemmal staining

HAL domain(s) :

Sciences du Vivant [q-bio]

English abstract : [en]

Histological analysis of skeletal muscle is of major interest for understanding its behavior in different pathophysiological conditions, such as the response to different environments or myopathies. In this context, many ...
Show more >Histological analysis of skeletal muscle is of major interest for understanding its behavior in different pathophysiological conditions, such as the response to different environments or myopathies. In this context, many software programs have been developed to perform automated high-content analysis. We created MuscleJ, a macro that runs in ImageJ/Fiji on batches of images. MuscleJ is a multianalysis tool that initially allows the analysis of muscle fibers, capillaries, and satellite cells. Since its creation, it has been used in many studies, and we have further developed the software and added new features, which are presented in this article. We converted the macro into a Java-language plugin with an improved user interface. MuscleJ2 provides quantitative analysis of fibrosis, vascularization, and cell phenotype in whole muscle sections. It also performs analysis of the peri-myonuclei, the individual capillaries, and any staining in the muscle fibers, providing accurate quantification within regional sublocalizations of the fiber. A multicartography option allows users to visualize multiple results simultaneously. The plugin is freely available to the muscle science community.Show less >

Language :

Anglais

Peer reviewed article :

Oui

Audience :

Internationale

Popular science :

Non

ANR Project :

EGID Diabetes Pole
Développment d'une infrastructure française distribuée coordonnée

Collections :

• Récepteurs nucléaires, Maladies Cardiovasculaires et Diabète (RNMCD) - U1011

Source :

Harvested from HAL