Bone diagenesis at early stage followed-up during 12 months by Raman spectroscopy

Falgayrac, Guillaume; Vitale, Raffaele; Delannoy, Yann; Olejnik, Cecile; Duponchel, Ludovic; Colard, Thomas

Document type :

Autre communication scientifique (congrès sans actes - poster - séminaire...)

Title :

Bone diagenesis at early stage followed-up during 12 months by Raman spectroscopy

Author(s) :

Falgayrac, Guillaume [Auteur]

Marrow Adiposity & Bone Lab - Adiposité Médullaire et Os - ULR 4490 [MABLab]
Université de Lille
Vitale, Raffaele [Auteur]
Laboratoire Avancé de Spectroscopie pour les Intéractions la Réactivité et l'Environnement - UMR 8516 [LASIRE]
Delannoy, Yann [Auteur]

Marrow Adiposity & Bone Lab - Adiposité Médullaire et Os - ULR 4490 [MABLab]
Unité de Taphonomie médico-légale et Anatomie - ULR 7367 [UTML&A]
Olejnik, Cecile [Auteur]

Université de Lille
Faculté de Chirurgie Dentaire
Marrow Adiposity & Bone Lab - Adiposité Médullaire et Os - ULR 4490 [MABLab]
Duponchel, Ludovic [Auteur]

Laboratoire Avancé de Spectroscopie pour les Intéractions la Réactivité et l'Environnement - UMR 8516 [LASIRE]
Colard, Thomas [Auteur]

Marrow Adiposity & Bone Lab - Adiposité Médullaire et Os - ULR 4490 [MABLab]

Conference title :

14th ICCBMT 2023

City :

Oosterbeek

Country :

Pays-Bas

Start date of the conference :

2023-10-23

HAL domain(s) :

Sciences du Vivant [q-bio]

English abstract : [en]

IntroductionFor the forensic anthropologists, bone remains encapsulate many features of the individual’s life history. The study of post-mortem alteration of bone is needed to differentiate the diagenetic processes from ...
Show more >IntroductionFor the forensic anthropologists, bone remains encapsulate many features of the individual’s life history. The study of post-mortem alteration of bone is needed to differentiate the diagenetic processes from the physiological or pathological processes. The bone diagenesis is defined as all interactions that occur between the bone and the burial environment [1]. Diagenetic mechanisms are complex especially in the early stages because numerous processes take place which depend on the burial environment (weather, soil, scavengers, …). Numerous studies focused on the evaluation of the diagenetic processes over archaeological periods, but few focused over forensic period [2]. PurposeThe aim of this study is to evaluate the molecular alterations induced by diagenesis on human bone during 12 months using Raman microspectroscopy. MethodsSix ribs from 6 subjects were placed in burial environment which consists in a plastic bin filled with clay soil from north of France. Plastic bins were placed outside and sheltered. Each month, a bone slice (<5 mm) was sampled from each rib. The remaining ribs are placed back in their burial environment. This procedure was repeated each month during 12 months. The bone slices were analysed on Raman microscope LabRAM (HR800) and equipped with a 785 nm laser diode. Forty spectra were acquired per rib and per month. The composition was assessed by 5 physico-chemical parameters calculated from Raman spectra.ResultsThe mineral/organic ratio and the carbonation type-B are decreased at a rate of -0.02 (p=0.015) and -0.03×10-3 (p<0.001) per month, respectively. The crystallinity is increased at a rate of +0.04×10-3 (p<0.001) per month. The modifications observed in the mineral matrix were in agreement with previous studies. The hydroxyproline/proline ratio is not modified. The collagen cross-links are decreased at the rate of -0.012 (p < 0.001) per month. The collagen cross-links give an assessment of the bonds that contribute to the stabilization of the helicoidal structure of collagen fibrils. This finding is in agreement with the mathematical simulation of the collagen hydrolysis [3]. ConclusionIn our conditions, the mechanism of diagenesis is driven by the hydrolysis of the collagen for bone remains buried during 12 months. References:[1] R.E.M. Hedges, Archaeometry, 44 (2002) 319[2] N. Procopio, C.A. Mein, S. Starace, A. Bonicelli, A. Williams, Biology, 10 (2021).[3] M.J. Collins, M.S. Riley, A.M. Child, G. Turner-Walker, J. Archaeol.Science, 22 (1995)Show less >

Language :

Anglais

Peer reviewed article :

Oui

Audience :

Internationale

Popular science :

Non

Collections :

Unité de Taphonomie Médico-Légale & d’Anatomie (UTMLA) - ULR 7367

Source :

Harvested from HAL

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