Species Identification and In Vitro ...
Type de document :
Article dans une revue scientifique: Article original
DOI :
URL permanente :
Titre :
Species Identification and In Vitro Antifungal Susceptibility of Paecilomyces/Purpureocillium Species Isolated from Clinical Respiratory Samples: A Multicenter Study
Auteur(s) :
Monpierre, Lorra [Auteur]
Aït-Ammar, Nawel [Auteur]
Valsecchi, Isabel [Auteur]
Normand, Anne-Cécile [Auteur]
Guitard, Juliette [Auteur]
Riat, Arnaud [Auteur]
Huguenin, Antoine [Auteur]
Bonnal, Christine [Auteur]
Sendid, Boualem [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576
Hasseine, Lilia [Auteur]
Raberin, Hélène [Auteur]
Dehais, Marion [Auteur]
Ranque, Stéphane [Auteur]
Hennequin, Christophe [Auteur]
Piarroux, Renaud [Auteur]
Dannaoui, Eric [Auteur]
Botterel, Françoise [Auteur]
Aït-Ammar, Nawel [Auteur]
Valsecchi, Isabel [Auteur]
Normand, Anne-Cécile [Auteur]
Guitard, Juliette [Auteur]
Riat, Arnaud [Auteur]
Huguenin, Antoine [Auteur]
Bonnal, Christine [Auteur]
Sendid, Boualem [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576
Hasseine, Lilia [Auteur]
Raberin, Hélène [Auteur]
Dehais, Marion [Auteur]
Ranque, Stéphane [Auteur]
Hennequin, Christophe [Auteur]
Piarroux, Renaud [Auteur]
Dannaoui, Eric [Auteur]
Botterel, Françoise [Auteur]
Titre de la revue :
Journal of Fungi
Nom court de la revue :
JoF
Numéro :
8
Pagination :
684
Éditeur :
MDPI
Date de publication :
2022-06-29
ISSN :
2309-608X
Mot(s)-clé(s) en anglais :
Paecilomyces variotii
Paecilomyces maximus
Purpureocillium lilacinum
antifungal susceptibility testing
molecular identification
MALDI-TOF mass spectrometry
MSI 2
Paecilomyces maximus
Purpureocillium lilacinum
antifungal susceptibility testing
molecular identification
MALDI-TOF mass spectrometry
MSI 2
Discipline(s) HAL :
Sciences du Vivant [q-bio]
Résumé en anglais : [en]
Paecilomyces spp. are emerging fungal pathogens, where Paecilomyces lilacinus and Paecilomyces variotii are the most reported species. Taxonomic and phylogenetic revisions in this genus have shown that P. variotii represents ...
Lire la suite >Paecilomyces spp. are emerging fungal pathogens, where Paecilomyces lilacinus and Paecilomyces variotii are the most reported species. Taxonomic and phylogenetic revisions in this genus have shown that P. variotii represents a species complex, whereas P. lilacinus is related to another genus called Purpureocillium. The aims of this study were to identify clinical isolates of Paecilomyces spp. at the species level, and to determine their antifungal susceptibility profiles. 70 clinical Paecilomyces spp. isolates were identified by MALDI-TOF Mass Spectrometry (MS) and by multilocus rDNA genes sequencing including ITS and the D1/D2 genes. Among the 70 Paecilomyces spp. isolates, 28 were identified as P. lilacinum, 26 as P. variotii stricto sensu, and 16 as P. maximus. For antifungal susceptibility testing, Minimal Inhibitory Concentrations (MICs) or Minimal Effective Concentrations (MECs) were determined for 8 antifungals. All P. lilacinum isolates had high MICs and MECs of amphotericin B and echinocandins, respectively, unlike P. variotii and P. maximus. For azole drugs, MICs were molecule- and species- dependent. The differences in in vitro susceptibility to antifungals underline the importance of accurate species identification. The MALDI–TOF MS can be a good alternative in routine laboratory to ensure fast identification of Paecilomyces spp. and P. lilacinum.Lire moins >
Lire la suite >Paecilomyces spp. are emerging fungal pathogens, where Paecilomyces lilacinus and Paecilomyces variotii are the most reported species. Taxonomic and phylogenetic revisions in this genus have shown that P. variotii represents a species complex, whereas P. lilacinus is related to another genus called Purpureocillium. The aims of this study were to identify clinical isolates of Paecilomyces spp. at the species level, and to determine their antifungal susceptibility profiles. 70 clinical Paecilomyces spp. isolates were identified by MALDI-TOF Mass Spectrometry (MS) and by multilocus rDNA genes sequencing including ITS and the D1/D2 genes. Among the 70 Paecilomyces spp. isolates, 28 were identified as P. lilacinum, 26 as P. variotii stricto sensu, and 16 as P. maximus. For antifungal susceptibility testing, Minimal Inhibitory Concentrations (MICs) or Minimal Effective Concentrations (MECs) were determined for 8 antifungals. All P. lilacinum isolates had high MICs and MECs of amphotericin B and echinocandins, respectively, unlike P. variotii and P. maximus. For azole drugs, MICs were molecule- and species- dependent. The differences in in vitro susceptibility to antifungals underline the importance of accurate species identification. The MALDI–TOF MS can be a good alternative in routine laboratory to ensure fast identification of Paecilomyces spp. and P. lilacinum.Lire moins >
Langue :
Anglais
Comité de lecture :
Oui
Audience :
Internationale
Vulgarisation :
Non
Établissement(s) :
Université de Lille
CNRS
CNRS
Équipe(s) de recherche :
Glycobiology in fungal Pathogenesis and Clinical Applications
Date de dépôt :
2024-02-26T15:36:18Z
2024-03-01T09:12:10Z
2024-03-01T09:12:10Z
Fichiers
- P22.45 Boualem Sendid - JOF 2022.pdf
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