Title :

Species Identification and In Vitro Antifungal Susceptibility of Paecilomyces/Purpureocillium Species Isolated from Clinical Respiratory Samples: A Multicenter Study

Author(s) :

Monpierre, Lorra [Auteur]
Aït-Ammar, Nawel [Auteur]
Valsecchi, Isabel [Auteur]
Normand, Anne-Cécile [Auteur]
Guitard, Juliette [Auteur]
Riat, Arnaud [Auteur]
Huguenin, Antoine [Auteur]
Bonnal, Christine [Auteur]
Sendid, Boualem [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576
Hasseine, Lilia [Auteur]
Raberin, Hélène [Auteur]
Dehais, Marion [Auteur]
Ranque, Stéphane [Auteur]
Hennequin, Christophe [Auteur]
Piarroux, Renaud [Auteur]
Dannaoui, Eric [Auteur]
Botterel, Françoise [Auteur]

Journal title :

Journal of Fungi

Abbreviated title :

JoF

Volume number :

8

Pages :

684

Publisher :

MDPI

Publication date :

2022-06-29

ISSN :

2309-608X

English keyword(s) :

Paecilomyces variotii
Paecilomyces maximus
Purpureocillium lilacinum
antifungal susceptibility testing
molecular identification
MALDI-TOF mass spectrometry
MSI 2

HAL domain(s) :

Sciences du Vivant [q-bio]

English abstract : [en]

Paecilomyces spp. are emerging fungal pathogens, where Paecilomyces lilacinus and Paecilomyces variotii are the most reported species. Taxonomic and phylogenetic revisions in this genus have shown that P. variotii represents ...
Show more >Paecilomyces spp. are emerging fungal pathogens, where Paecilomyces lilacinus and Paecilomyces variotii are the most reported species. Taxonomic and phylogenetic revisions in this genus have shown that P. variotii represents a species complex, whereas P. lilacinus is related to another genus called Purpureocillium. The aims of this study were to identify clinical isolates of Paecilomyces spp. at the species level, and to determine their antifungal susceptibility profiles. 70 clinical Paecilomyces spp. isolates were identified by MALDI-TOF Mass Spectrometry (MS) and by multilocus rDNA genes sequencing including ITS and the D1/D2 genes. Among the 70 Paecilomyces spp. isolates, 28 were identified as P. lilacinum, 26 as P. variotii stricto sensu, and 16 as P. maximus. For antifungal susceptibility testing, Minimal Inhibitory Concentrations (MICs) or Minimal Effective Concentrations (MECs) were determined for 8 antifungals. All P. lilacinum isolates had high MICs and MECs of amphotericin B and echinocandins, respectively, unlike P. variotii and P. maximus. For azole drugs, MICs were molecule- and species- dependent. The differences in in vitro susceptibility to antifungals underline the importance of accurate species identification. The MALDI–TOF MS can be a good alternative in routine laboratory to ensure fast identification of Paecilomyces spp. and P. lilacinum.Show less >

Language :

Anglais

Peer reviewed article :

Oui

Audience :

Internationale

Popular science :

Non

Administrative institution(s) :

Université de Lille
CNRS

Collections :

• Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576

Research team(s) :

Glycobiology in fungal Pathogenesis and Clinical Applications

Submission date :

2024-02-26T15:36:18Z
2024-03-01T09:12:10Z