Title :

Multicenter Evaluation of an ELISA for the Detection of Cryptosporidium spp. Antigen in Clinical Human Stool Samples

Author(s) :

Razakandrainibe, Romy [Auteur]
Université de Rouen Normandie [UNIROUEN]
Mérat, Célia [Auteur]
Centre Hospitalier Universitaire de Nantes = Nantes University Hospital [CHU Nantes]
Kapel, Nathalie [Auteur]
Université Paris Cité [UPCité]
Sautour, Marc [Auteur]
CHU Dijon
Guyot, Karine [Auteur]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
Gargala, Gilles [Auteur]
Université de Rouen Normandie [UNIROUEN]
Ballet, Jean-Jacques [Auteur]
Université de Rouen Normandie [UNIROUEN]
Le Pape, Patrice [Auteur]
Centre Hospitalier Universitaire de Nantes = Nantes University Hospital [CHU Nantes]
Network, French Cryptosporidiosis [Auteur]
Dalle, Frédéric [Auteur]
CHU Dijon
Favennec, Loïc [Auteur]
Université de Rouen Normandie [UNIROUEN]

Journal title :

Microorganisms

Pages :

209

Publisher :

MDPI

Publication date :

2021-01-20

ISSN :

2076-2607

HAL domain(s) :

Sciences du Vivant [q-bio]

English abstract : [en]

Human cryptosporidiosis remains underdiagnosed, and rapid/accurate diagnosis is of clinical importance. Diagnosis of the Cryptosporidium oocyst in stool samples by conventional microscopy is labor-intensive, time-consuming, ...
Show more >Human cryptosporidiosis remains underdiagnosed, and rapid/accurate diagnosis is of clinical importance. Diagnosis of the Cryptosporidium oocyst in stool samples by conventional microscopy is labor-intensive, time-consuming, and requires skillful experience. Thus, we aimed to evaluate the usefulness of a coproantigen enzyme-linked immunosorbent assay (ELISA) test in detecting Cryptosporidium spp. from fecal specimens. For this aim, we evaluated the performances of a commercial ELISA (CoproELISA Cryptosporidium kit, Savyon Diagnostics, Israel) for the detection of Cryptosporidium spp. in random clinical stool samples through a multicenter study. The sensitivity and specificity for coproantigen ELISA were 98.86% and 94.32%, respectively. The coproantigen ELISA results indicate that the simple, rapid, reliable, and standardized immunoassay test is sensitive and specific for routine diagnosis, and may be useful for large-scale epidemiological studies of cryptosporidiosis.Show less >

Language :

Anglais

Peer reviewed article :

Oui

Audience :

Internationale

Popular science :

Non

Collections :

• Centre d'Infection et d'Immunité de Lille (CIIL) - U1019 - UMR 9017

Source :

Harvested from HAL