Title :

Raman microspectroscopy reveals unsaturation heterogeneity at the lipid droplet level and validates an in vitro model of bone marrow adipocyte subtypes

Author(s) :

Tratwal, Josefine []
Université de Lausanne = University of Lausanne [UNIL]
Falgayrac, Guillaume []
201443|||Marrow Adiposity & Bone Lab - Adiposité Médullaire et Os - ULR 4490 [MABLab (ex-pmoi)] (VALID)
During, Alexandrine [Auteur]
201443|||Marrow Adiposity & Bone Lab - Adiposité Médullaire et Os - ULR 4490 [MABLab (ex-pmoi)] (VALID)
Bertheaume, Nicolas [Auteur]
Marrow Adiposity & Bone Lab - Adiposité Médullaire et Os - ULR 4490 [MABLab]
Bataclan, Charles [Auteur]
Tavakol, Daniel [Auteur]
Campos, Vasco [Auteur]
Duponchel, Ludovic [Auteur]
Laboratoire Avancé de Spectroscopie pour les Intéractions la Réactivité et l'Environnement (LASIRE) - UMR 8516
Daley, George [Auteur]
Dana-Farber Cancer Institute [Boston]
Boston Children's Hospital
Penel, Guillaume [Auteur]
201443|||Marrow Adiposity & Bone Lab - Adiposité Médullaire et Os - ULR 4490 [MABLab (ex-pmoi)] (VALID)
Chauveau, Christophe []
Marrow Adiposity & Bone Lab (MABLab) - ULR 4490
Naveiras, Olaia [Auteur correspondant]

Journal title :

Frontiers in Endocrinology

Volume number :

13

Publisher :

Frontiers

Publication date :

2022-10-01

ISSN :

1664-2392

English keyword(s) :

fatty acid unsaturation (fatty acid) OP9 cell lipid droplet diameter Regulated constitutive Stroma Bone marrow stromal cell (BMSC)
fatty acid
unsaturation (fatty acid)
OP9 cell
lipid droplet diameter
Regulated
constitutive
Stroma
Bone marrow stromal cell (BMSC)

HAL domain(s) :

Sciences du Vivant [q-bio]

English abstract : [en]

Bone marrow adipocytes (BMAds) constitute the most abundant stromalcomponent of adult human bone marrow. Two subtypes of BMAds havebeen described, the more labile regulated adipocytes (rBMAds) and the morestable constitutive ...
Show more >Bone marrow adipocytes (BMAds) constitute the most abundant stromalcomponent of adult human bone marrow. Two subtypes of BMAds havebeen described, the more labile regulated adipocytes (rBMAds) and the morestable constitutive adipocytes (cBMAds), which develop earlier in life and aremore resilient to environmental and metabolic disruptions. In vivo, rBMAds areenriched in saturated fatty acids, contain smaller lipid droplets (LDs) and morereadily provide hematopoietic support than their cBMAd counterparts. Mousemodels have been used for BMAds research, but isolation of primary BMAdspresents many challenges, and thus in vitro models remain the current standardto study nuances of adipocyte differentiation. No in vitro model has yet beendescribed for the study of rBMAds/cBMAds. Here, we present an in vitro modelof BM adipogenesis with differential rBMAd and cBMAd-like characteristics. Weused OP9 BM stromal cells derived from a (C57BL/6xC3H)F2-op/op mouse,which have been extensively characterized as feeder layer for hematopoiesisresearch. We observed similar canonical adipogenesis transcriptionalsignatures for spontaneously-differentiated (sOP9) and induced (iOP9)cultures, while fatty acid composition and desaturase expression of Scd1 andFads2 differed at the population level. To resolve differences at the singleadipocyte level we tested Raman microspectroscopy and show it constitutes ahigh-resolution method for studying adipogenesis in vitro in a label-freemanner, with resolution to individual LDs. We found sOP9 adipocytes have lower unsaturation ratios, smaller LDs and higher hematopoietic support thaniOP9 adipocytes, thus functionally resembling rBMAds, while iOP9 moreclosely resembled cBMAds. Validation in human primary samples confirmed ahigher unsaturation ratio for lipids extracted from stable cBMAd-rich sites(femoral head upon hip-replacement surgery) versus labile rBMAds (iliaccrest after chemotherapy). As a result, the 16:1/16:0 fatty acid unsaturationratio, which was already shown to discriminate BMAd subtypes in rabbit and ratmarrow, was validated to discriminate cBMAds from rBMAd in both the OP9model in vitro system and in human samples. We expect our model will beuseful for cBMAd and rBMAd studies, particularly where isolation of primaryBMAds is a limiting step.Show less >

Language :

Anglais

Peer reviewed article :

Oui

Audience :

Internationale

Popular science :

Non

Administrative institution(s) :

Université de Lille
CNRS

Collections :

• Laboratoire Avancé de Spectroscopie pour les Intéractions la Réactivité et l'Environnement (LASIRE) - UMR 8516
• Marrow Adiposity & Bone Lab (MABLab) - ULR 4490

Submission date :

2024-02-27T11:18:17Z