Title :

Polymorphic microsatellite markers demonstrate hybridization and interspecific gene flow between lumbricid earthworm species, Eisenia andrei and E. fetida.

Author(s) :

Jaskulak, Marta [Auteur]
Laboratoire de Génie Civil et Géo-Environnement (LGCgE) - ULR 4515 [LGCgE]
Rorat, Agnieszka [Auteur]
Laboratoire de Génie Civil et Géo-Environnement (LGCgE) - ULR 4515 [LGCgE]
Vandenbulcke, Franck [Auteur]
Laboratoire Génie Civil et géo-Environnement (LGCgE) - ULR 4515
Pauwels, Maxime [Auteur]
Évolution, Écologie et Paléontologie (Evo-Eco-Paleo) - UMR 8198 [Evo-Eco-Paléo (EEP)]
Laboratoire de Génie Civil et Géo-Environnement (LGCgE) - ULR 4515 [LGCgE]
Grzmil, P. [Auteur]
Plytycz, B. [Auteur]

Journal title :

PLoS ONE

Abbreviated title :

PLoS One

Volume number :

17

Pages :

e0262493

Publication date :

2022-02-22

ISSN :

1932-6203

HAL domain(s) :

Sciences de l'ingénieur [physics]/Génie civil

English abstract : [en]

The lumbricid earthworms Eisenia andrei (Ea) and E. fetida (Ef) have been used as model organisms for studies on hybridization. Previously they have been identified by species specific sequences of the mitochondrial COI ...
Show more >The lumbricid earthworms Eisenia andrei (Ea) and E. fetida (Ef) have been used as model organisms for studies on hybridization. Previously they have been identified by species specific sequences of the mitochondrial COI gene of maternal origin (‘a’ or ‘f’) and the nuclear 28S gene of maternal/paternal origin (‘A’ or ‘F’). In experimental crosses, these hermaphroditic species produce progeny of genotypes Ea (aAA), Ef (fFF) and hybrids (aAF and fFA) originating by self-fertilization or cross-fertilization. To facilitate studies on new aspects of the breeding biology and hybridization of earthworms, polymorphic microsatellite markers were developed based on 12 Ea and 12 Ef specimens and validated on DNA samples extracted from 24 genotyped specimens (aAA, fFF, aAF and fFA) from three laboratory-raised families and 10 of them were applied in the present study. The results indicate that microsatellite markers are valuable tools for tracking interspecific gene flow between these species.Show less >

Language :

Anglais

Peer reviewed article :

Oui

Audience :

Internationale

Popular science :

Non

Administrative institution(s) :

Université de Lille
CNRS

Collections :

• Évolution, Écologie et Paléontologie (Evo Eco Paleo) - UMR 8198
• Laboratoire Génie Civil et géo-Environnement (LGCgE) - ULR 4515

Submission date :

2024-02-28T22:32:31Z
2024-03-20T09:15:10Z