Title :

Comparison of Three β-Glucan Tests for the Diagnosis of Invasive Candidiasis in Intensive Care Units

Author(s) :

Kritikos, A. [Auteur]
Université de Lausanne = University of Lausanne [UNIL]
Caruana, G. [Auteur]
Université de Lausanne = University of Lausanne [UNIL]
Poissy, Julien [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576
Mamin, A. [Auteur]
Université de Lausanne = University of Lausanne [UNIL]
Bachmann, D. [Auteur]
Université de Lausanne = University of Lausanne [UNIL]
Pagani, J. L. [Auteur]
Université de Lausanne = University of Lausanne [UNIL]
Coste, A. T. [Auteur]
Université de Lausanne = University of Lausanne [UNIL]
Lamoth, F. [Auteur]
Université de Lausanne = University of Lausanne [UNIL]

Journal title :

Journal of clinical microbiology

Abbreviated title :

J Clin Microbiol

Volume number :

61

Publisher :

American Society for Microbiology

Publication date :

2023-02-22

ISSN :

0095-1137

English keyword(s) :

Candida
intensive care
intra-abdominal candidiasis
candidemia

HAL domain(s) :

Sciences du Vivant [q-bio]

English abstract : [en]

The (1!3)-b-D-glucan (BDG) is a marker of invasive fungal infection that can be detected in serum by different commercial kits. In this study, we compared the performance of the Fungitell assay (FA), the Fungitell STAT ...
Show more >The (1!3)-b-D-glucan (BDG) is a marker of invasive fungal infection that can be detected in serum by different commercial kits. In this study, we compared the performance of the Fungitell assay (FA), the Fungitell STAT assay (STAT), and the Wakob-glucan test (WA) for the diagnosis of invasive candidiasis (IC) in the intensive care unit (ICU). Patients for whom at least one BDG testing was required for a clinical suspicion of IC were retrospectively enrolled. A total of 85 serum samples from 56 patients were tested by the three BDG tests. The rate of IC was 23% (13/56) with a predominance of noncandidemic (intra-abdominal) IC. STAT and WA results exhibited overall good correlation with those obtained by FA (Spearman’s coefficient R = 0.90 and R = 0.89, respectively). For the recommended cutoffs of positivity, sensitivity and specificity for IC diagnosis were 77%/51% (FA, 80 pg/mL), 69%/53% (STAT, ratio 1.2), and 54%/65% (WA, 7 pg/mL), respectively. Optimal performance was obtained at 50 pg/mL (FA), ratio 1.3 (STAT), and 3.3 pg/mL (WA) with sensitivity/specificity of 85%/51%, 69%/57%, and 77%/58%, respectively. Overall, the three BDG tests showed comparable but limited performance in this setting with positive and negative predictive values for an estimated IC prevalence of 20% that were in the range of 30 to 35% and 85 to 95%, respectively.Show less >

Language :

Anglais

Audience :

Internationale

Popular science :

Non

Administrative institution(s) :

Université de Lille
CNRS

Collections :

• Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576

Research team(s) :

Glycobiology in fungal Pathogenesis and Clinical Applications

Submission date :

2024-03-01T10:31:58Z
2024-03-01T10:54:11Z