Title :

Testosterone‐androgen receptor: The steroid link inhibiting TRPM8‐mediated cold sensitivity

Author(s) :

Gkika, Dimitra []
Laboratoire de Physiologie Cellulaire - U 1003 [PHYCELL]
Hétérogénéité, Plasticité et Résistance aux Thérapies des Cancers = Cancer Heterogeneity, Plasticity and Resistance to Therapies - UMR 9020 - U 1277 [CANTHER]
Lolignier, Stéphane [Auteur]
Neuro-Dol [Neuro-Dol]
Institut Analgesia
Grolez, Guillaume [Auteur]
Laboratoire de Physiologie Cellulaire - U 1003 [PHYCELL]
Bavencoffe, Alexis [Auteur]
Laboratoire de Physiologie Cellulaire - U 1003 [PHYCELL]
Shapovalov, Georges [Auteur]
Laboratoire de Physiologie Cellulaire - U 1003 [PHYCELL]
Gordienko, Dmitri [Auteur]
Laboratoire de Physiologie Cellulaire - U 1003 [PHYCELL]
Site de Recherche Intégrée en Cancérologie [SIRIC-ONCOLille]
Kondratskyi, Artem [Auteur]
Laboratoire de Physiologie Cellulaire - U 1003 [PHYCELL]
Meleine, Mathieu [Auteur]
Neuro-Dol [Neuro-Dol]
Institut Analgesia
Prival, Laetitia [Auteur]
Neuro-Dol [Neuro-Dol]
Institut Analgesia
Chapuy, Eric [Auteur]
Neuro-Dol [Neuro-Dol]
Institut Analgesia
Etienne, Monique [Auteur]
Neuro-Dol [Neuro-Dol]
Institut Analgesia
Eschalier, Alain [Auteur]
Institut Analgesia
Neuro-Dol [Neuro-Dol]
Shuba, Yaroslav [Auteur]
Laboratoire de Physiologie Cellulaire - U 1003 [PHYCELL]
Skryma, Roman [Auteur]
Rôle des canaux ioniques membranaires et du calcium intracellulaire dans la physiopathologie de la prostate
Busserolles, Jérôme [Auteur]
Neuro-Dol [Neuro-Dol]
Institut Analgesia
Prevarskaya, Natalia [Auteur]
Laboratoire de Physiologie Cellulaire - U 1003 [PHYCELL]

Journal title :

FASEB Journal

Pages :

7483-7499

Publisher :

Federation of American Society of Experimental Biology

Publication date :

2020-06

ISSN :

0892-6638

English keyword(s) :

C-terminal tail
Nt
N-terminal tail
PLA
proximity ligation assay
Ct

HAL domain(s) :

Sciences du Vivant [q-bio]/Neurosciences [q-bio.NC]
Sciences du Vivant [q-bio]/Sciences pharmaceutiques/Pharmacologie

English abstract : [en]

Recent studies have revealed gender differences in cold perception, and pointed to a possible direct action of testosterone (TST) on the cold-activated TRPM8 (Transient Receptor Potential Melastatin Member 8) channel. ...
Show more >Recent studies have revealed gender differences in cold perception, and pointed to a possible direct action of testosterone (TST) on the cold-activated TRPM8 (Transient Receptor Potential Melastatin Member 8) channel. However, the mechanisms by which TST influences TRPM8-mediated sensory functions remain elusive. Here, we show that TST inhibits TRPM8-mediated mild-cold perception through the nonca-nonical engagement of the Androgen Receptor (AR). Castration of both male rats and mice increases sensitivity to mild cold, and this effect depends on the presence of intact TRPM8 and AR. TST in nanomolar concentrations suppresses whole-cell TRPM8-mediated currents and single-channel activity in native dorsal root ganglion (DRG) neurons and HEK293 cells co-expressing recombinant TRPM8 and AR, but not TRPM8 alone. AR cloned from rat DRGs shows no difference from standard AR. However, biochemical assays and confocal imaging reveal the presence of AR on the cell surface and its interaction with TRPM8 in response to TST, leading to an inhibition of channel activity.Show less >

Language :

Anglais

Peer reviewed article :

Oui

Audience :

Internationale

Popular science :

Non

Collections :

• Cancer Heterogeneity, Plasticity and Resistance to Therapies (CANTHER) - UMR 9020 - UMR 1277

Source :

Harvested from HAL