Titre :

Testosterone‐androgen receptor: The steroid link inhibiting TRPM8‐mediated cold sensitivity

Auteur(s) :

Gkika, Dimitra []
Hétérogénéité, Plasticité et Résistance aux Thérapies des Cancers = Cancer Heterogeneity, Plasticity and Resistance to Therapies - UMR 9020 - U 1277 [CANTHER]
Laboratoire de Physiologie Cellulaire - U 1003 [PHYCELL]
Lolignier, Stéphane [Auteur]
Institut Analgesia
Neuro-Dol [Neuro-Dol]
Grolez, Guillaume [Auteur]
Laboratoire de Physiologie Cellulaire - U 1003 [PHYCELL]
Bavencoffe, Alexis [Auteur]
Laboratoire de Physiologie Cellulaire - U 1003 [PHYCELL]
Shapovalov, Georges [Auteur]
Laboratoire de Physiologie Cellulaire - U 1003 [PHYCELL]
Gordienko, Dmitri [Auteur]
Site de Recherche Intégrée en Cancérologie [SIRIC-ONCOLille]
Laboratoire de Physiologie Cellulaire - U 1003 [PHYCELL]
Kondratskyi, Artem [Auteur]
Laboratoire de Physiologie Cellulaire - U 1003 [PHYCELL]
Meleine, Mathieu [Auteur]
Institut Analgesia
Neuro-Dol [Neuro-Dol]
Prival, Laetitia [Auteur]
Institut Analgesia
Neuro-Dol [Neuro-Dol]
Chapuy, Eric [Auteur]
Institut Analgesia
Neuro-Dol [Neuro-Dol]
Etienne, Monique [Auteur]
Institut Analgesia
Neuro-Dol [Neuro-Dol]
Eschalier, Alain [Auteur]
Neuro-Dol [Neuro-Dol]
Institut Analgesia
Shuba, Yaroslav [Auteur]
Laboratoire de Physiologie Cellulaire - U 1003 [PHYCELL]
Skryma, Roman [Auteur]
Rôle des canaux ioniques membranaires et du calcium intracellulaire dans la physiopathologie de la prostate
Busserolles, Jérôme [Auteur]
Institut Analgesia
Neuro-Dol [Neuro-Dol]
Prevarskaya, Natalia [Auteur]
Laboratoire de Physiologie Cellulaire - U 1003 [PHYCELL]

Titre de la revue :

FASEB Journal

Pagination :

7483-7499

Éditeur :

Federation of American Society of Experimental Biology

Date de publication :

2020-06

ISSN :

0892-6638

Mot(s)-clé(s) en anglais :

C-terminal tail
Nt
N-terminal tail
PLA
proximity ligation assay
Ct

Discipline(s) HAL :

Sciences du Vivant [q-bio]/Neurosciences [q-bio.NC]
Sciences du Vivant [q-bio]/Sciences pharmaceutiques/Pharmacologie

Résumé en anglais : [en]

Recent studies have revealed gender differences in cold perception, and pointed to a possible direct action of testosterone (TST) on the cold-activated TRPM8 (Transient Receptor Potential Melastatin Member 8) channel. ...
Lire la suite >Recent studies have revealed gender differences in cold perception, and pointed to a possible direct action of testosterone (TST) on the cold-activated TRPM8 (Transient Receptor Potential Melastatin Member 8) channel. However, the mechanisms by which TST influences TRPM8-mediated sensory functions remain elusive. Here, we show that TST inhibits TRPM8-mediated mild-cold perception through the nonca-nonical engagement of the Androgen Receptor (AR). Castration of both male rats and mice increases sensitivity to mild cold, and this effect depends on the presence of intact TRPM8 and AR. TST in nanomolar concentrations suppresses whole-cell TRPM8-mediated currents and single-channel activity in native dorsal root ganglion (DRG) neurons and HEK293 cells co-expressing recombinant TRPM8 and AR, but not TRPM8 alone. AR cloned from rat DRGs shows no difference from standard AR. However, biochemical assays and confocal imaging reveal the presence of AR on the cell surface and its interaction with TRPM8 in response to TST, leading to an inhibition of channel activity.Lire moins >

Langue :

Anglais

Comité de lecture :

Oui

Audience :

Internationale

Vulgarisation :

Non

Collections :

• Cancer Heterogeneity, Plasticity and Resistance to Therapies (CANTHER) - UMR 9020 - UMR 1277

Source :

Harvested from HAL