Title :

Senescence Induced by UVB in Keratinocytes Impairs Amino Acids Balance

Author(s) :

Bauwens, Emilie [Auteur]
Parée, Tom [Auteur]
Meurant, Sébastien [Auteur]
Bouriez, Inès [Auteur]
Hannart, Clotilde [Auteur]
Wéra, Anne-Catherine [Auteur]
Khelfi, Alexis [Auteur]
Fattaccioli, Antoine [Auteur]
Burteau, Sophie [Auteur]
Demazy, Catherine [Auteur]
Fransolet, Maude [Auteur]
De Schutter, Clémentine [Auteur]
Hétérogénéité, Plasticité et Résistance aux Thérapies des Cancers = Cancer Heterogeneity, Plasticity and Resistance to Therapies - UMR 9020 - U 1277 [CANTHER]
Martin, Nathalie [Auteur]
Hétérogénéité, Plasticité et Résistance aux Thérapies des Cancers = Cancer Heterogeneity, Plasticity and Resistance to Therapies - UMR 9020 - U 1277 [CANTHER]
Théry, Julien [Auteur]
Decanter, Gauthier [Auteur]
Penel, Nicolas [Auteur]
Bury, Marina [Auteur]
Pluquet, Olivier [Auteur]
Hétérogénéité, Plasticité et Résistance aux Thérapies des Cancers = Cancer Heterogeneity, Plasticity and Resistance to Therapies - UMR 9020 - U 1277 [CANTHER]
Garmyn, Marjan [Auteur]
Debacq-Chainiaux, Florence [Auteur]

Journal title :

Journal of Investigative Dermatology

Pages :

554-565.e9

Publisher :

Nature Publishing Group

Publication date :

2023-04

ISSN :

0022-202X

HAL domain(s) :

Sciences du Vivant [q-bio]

English abstract : [en]

Skin is one of the most exposed organs to external stress. Namely, UV rays are the most harmful stress that could induce important damage leading to skin aging and cancers. At the cellular level, senescence is observed in ...
Show more >Skin is one of the most exposed organs to external stress. Namely, UV rays are the most harmful stress that could induce important damage leading to skin aging and cancers. At the cellular level, senescence is observed in several skin cell types and contributes to skin aging. However, the origin of skin senescent cells is still unclear but is probably related to exposure to stresses. In this work, we developed an in vitro model of UVBinduced premature senescence in normal human epidermal keratinocytes. UVB-induced senescent keratinocytes display a common senescent phenotype resulting in an irreversible cell cycle arrest, an increase in the proportion of senescence-associated b-galactosidase-positive cells, unrepaired DNA damage, and a long-term DNA damage response activation. Moreover, UVB-induced senescent keratinocytes secrete senescenceassociated secretory phenotype factors that influence cutaneous squamous cell carcinoma cell migration. Finally, a global transcriptomic study highlighted that senescent keratinocytes present a decrease in the expression of several amino acid transporters, which is associated with reduced intracellular levels of glycine, alanine, and leucine. Interestingly, the chemical inhibition of the glycine transporter SLC6A9/Glyt1 triggers senescence features.Show less >

Language :

Anglais

Popular science :

Non

Collections :

• Cancer Heterogeneity, Plasticity and Resistance to Therapies (CANTHER) - UMR 9020 - UMR 1277

Source :

Harvested from HAL

Submission date :

2024-03-20T03:20:13Z