Title :

Interlaboratory evaluation of Mucorales PCR assays for testing serum specimens: A study by the fungal PCR Initiative and the Modimucor study group

Author(s) :

Rocchi, S [Auteur]
Service de parasitologie et mycologie [CHRU de Besançon]
Laboratoire Chrono-environnement (UMR 6249) [LCE]
Scherer, E [Auteur]
Service de parasitologie et mycologie [CHRU de Besançon]
Laboratoire Chrono-environnement (UMR 6249) [LCE]
Mengoli, C [Auteur]
Università degli Studi di Padova = University of Padua [Unipd]
Alanio, A [Auteur]
Université Paris Cité [UPCité]
Centre National de Référence Mycoses Invasives et Antifongiques - National Reference Center Invasive Mycoses & Antifungals [CNRMA]
Hôpitaux Universitaire Saint-Louis, Lariboisière, Fernand-Widal
Mycologie moléculaire - Molecular Mycology
Botterel, F [Auteur]
CHU Henri Mondor [Créteil]
École nationale vétérinaire d'Alfort [ENVA]
Université Paris-Est Créteil Val-de-Marne - Faculté de médecine [UPEC Médecine]
Bougnoux, M E [Auteur]
Biologie et Pathogénicité fongiques [BPF]
Hôpital Necker - Enfants Malades [AP-HP]
Bretagne, S [Auteur]
Mycologie moléculaire
Université Paris Cité [UPCité]
Centre National de Référence Mycoses Invasives et Antifongiques - National Reference Center Invasive Mycoses & Antifungals [CNRMA]
Hôpitaux Universitaire Saint-Louis, Lariboisière, Fernand-Widal
Cogliati, M [Auteur]
Università degli Studi di Milano = University of Milan [UNIMI]
Cornu, Marjorie [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Dalle, F [Auteur]
Laboratoire de parasitologie mycologie (CHU de Dijon)
Damiani, C [Auteur]
Laboratoire de parasitologie et de mycologie médicales [CHU Amiens]
Denis, J [Auteur]
Laboratoire de Parasitologie et de Mycologie Médicale [Strasbourg]
Fuchs, S [Auteur]
Leopold Franzens Universität Innsbruck - University of Innsbruck
Gits-Muselli, M [Auteur]
UFR Médecine [Santé] - Université Paris Cité [UFR Médecine UPCité]
Hôpitaux Universitaire Saint-Louis, Lariboisière, Fernand-Widal
Hagen, F [Auteur]
University Medical Center [Utrecht] [UMCU]
Universiteit Utrecht / Utrecht University [Utrecht]
Halliday, C [Auteur]
Hare, R [Auteur]
Statens Serum Institut [Copenhagen]
Iriart, X [Auteur]
Centre de Physiopathologie Toulouse Purpan [CPTP]
Service de Parasitologie et Mycologie [CHU Toulouse]
Klaassen, C [Auteur]
Erasmus University Medical Center [Rotterdam] [Erasmus MC]
Lackner, M [Auteur]
Innsbruck Medical University = Medizinische Universität Innsbruck [IMU]
Lengerova, M [Auteur]
University Hospital Brno
Letscher-Bru, V [Auteur]
Centre Hospitalier Universitaire [Strasbourg] [CHU Strasbourg]
Morio, F [Auteur]
Centre Hospitalier Universitaire de Nantes = Nantes University Hospital [CHU Nantes]
Nourrisson, C [Auteur]
CHU Clermont-Ferrand
Posch, W [Auteur]
Innsbruck Medical University = Medizinische Universität Innsbruck [IMU]
Sendid, Boualem [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Springer, J [Auteur]
University Hospital Wuerzburg / Universitäts­klinikum Würzburg
Willinger, B [Auteur]
Medizinische Universität Wien = Medical University of Vienna
White, P L [Auteur]
University Hospital of Wales [UHW]
Barnes, R A [Auteur]
Cruciani, M [Auteur]
Donnelly, J P [Auteur]
University of Texas Health Science Center at San Antonio [San Antonio, Tx, USA]
Loeffler, J [Auteur]
University Hospital Wuerzburg / Universitäts­klinikum Würzburg
Millon, L [Auteur]
Service de parasitologie et mycologie [CHRU de Besançon]
Laboratoire Chrono-environnement (UMR 6249) [LCE]

Journal title :

Medical Mycology

Volume number :

59

Pages :

126-138

Publisher :

Oxford University Press (OUP)

Publication date :

2020-06-13

HAL domain(s) :

Chimie/Chimie théorique et/ou physique

English abstract : [en]

Abstract Interlaboratory evaluations of Mucorales qPCR assays were developed to assess the reproducibility and performance of methods currently used. The participants comprised 12 laboratories from French ...
Show more >Abstract Interlaboratory evaluations of Mucorales qPCR assays were developed to assess the reproducibility and performance of methods currently used. The participants comprised 12 laboratories from French university hospitals (nine of them participating in the Modimucor study) and 11 laboratories participating in the Fungal PCR Initiative. For panel 1, three sera were each spiked with DNA from three different species (Rhizomucor pusillus, Lichtheimia corymbifera, Rhizopus oryzae). For panel 2, six sera with three concentrations of R. pusillus and L. corymbifera (1, 10, and 100 genomes/ml) were prepared. Each panel included a blind negative-control serum. A form was distributed with each panel to collect results and required technical information, including DNA extraction method, sample volume used, DNA elution volume, qPCR method, qPCR template input volume, qPCR total reaction volume, qPCR platform, and qPCR reagents used. For panel 1, assessing 18 different protocols, qualitative results (positive or negative) were correct in 97% of cases (70/72). A very low interlaboratory variability in Cq values (SD = 1.89 cycles) were observed. For panel 2 assessing 26 different protocols, the detection rates were high (77–100%) for 5/6 of spiked serum. There was a significant association between the qPCR platform and performance. However, certain technical steps and optimal combinations of factors may also impact performance. The good reproducibility and performance demonstrated in this study support the use of Mucorales qPCR as part of the diagnostic strategy for mucormycosis.Show less >

Language :

Anglais

Audience :

Internationale

Popular science :

Non

Administrative institution(s) :

Université de Lille
CNRS

Collections :

• Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576

Research team(s) :

Glycobiology in fungal Pathogenesis and Clinical Applications

Submission date :

2024-03-25T10:20:09Z
2024-03-27T08:57:55Z