Title :

Using capillary electrophoresis to identify Anopheline species in routine sampling sites

Author(s) :

Chabanol, Estelle [Auteur]
Ecole Doctorale 587 : Diversités, santé et développement en Amazonie [ED 587]
Université de Guyane [UG]
Institut Pasteur de la Guyane
Romoli, Ottavia [Auteur]
Institut Pasteur de la Guyane
Talaga, Stanislas [Auteur]
Institut Pasteur de la Guyane
Epelboin, Yanouk [Auteur]
Institut Pasteur de la Guyane
Heu, Katy [Auteur]
Institut Pasteur de la Guyane
Prévot, Ghislaine [Auteur]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
Université de Guyane [UG]
Gendrin, Mathilde [Auteur correspondant]
Département Parasites et Insectes vecteurs - Department of Parasites and Insect Vectors
Institut Pasteur de la Guyane

Journal title :

Ecology And Evolution

Pages :

10782

Publisher :

Wiley Open Access

Publication date :

2024-03-12

English keyword(s) :

Anopheles
capillary electrophoresis
French Guiana
ITS2
length polymorphism
mosquito
Nyssorhynchus
species identification

HAL domain(s) :

Sciences du Vivant [q-bio]/Biodiversité/Systématique, phylogénie et taxonomie
Sciences du Vivant [q-bio]/Biotechnologies

English abstract : [en]

Abstract In the Anopheles genus, various mosquito species are able to transmit the Plasmodium parasites responsible for malaria, while others are non‐vectors. In an effort to better understand the biology of Anopheles ...
Show more >Abstract In the Anopheles genus, various mosquito species are able to transmit the Plasmodium parasites responsible for malaria, while others are non‐vectors. In an effort to better understand the biology of Anopheles species and to quantify transmission risk in an area, the identification of mosquito species collected in the field is an essential but problematic task. Morphological identification requires expertise and cannot be checked after processing samples in a destructive treatment, while sequencing of numerous samples is costly. Here, we introduce a method of Species identification via Simple Observation Coupled with Capillary Electrophoresis Technology (SOCCET). This molecular technique of species identification is based on precise determination of ITS2 length combined with a simple visual observation, the colour of mosquito hindleg tip. DNA extracted from field‐collected Anopheles mosquitoes was amplified with universal Anopheles ITS2 primers and analysed with a capillary electrophoresis device, which precisely determines the size of the fragments. We defined windows of amplicon sizes combined with fifth hind tarsus colour, which allows discrimination of the major Anopheles species found in our collections. We validated our parameters via Sanger sequencing of ITS2 amplicons. Using the SOCCET method, we characterised the composition of Anopheles populations in five locations of French Guiana, where we detected a total of nine species. Anopheles braziliensis and Anopheles darlingi were detected in four locations each and represented 13 and 67% of our samples, respectively. The SOCCET method can be particularly useful when working with routine sampling sites with a moderate species diversity, that is, when the number of local species is too high to define species‐specific primers but low enough to avoid individual ITS2 sequencing. This tool will be of interest to evaluate local malaria transmission risk and this approach may be further implemented for other mosquito genera.Show less >

Language :

Anglais

Peer reviewed article :

Oui

Audience :

Internationale

Popular science :

Non

ANR Project :

Integrative Biology of Emerging Infectious Diseases
Remodelage du microbiote de moustique pour étudier son impact sur la transmission du paludisme
CEnter of the study of Biodiversity in Amazonia

Collections :

• Centre d'Infection et d'Immunité de Lille (CIIL) - U1019 - UMR 9017

Source :

Harvested from HAL