Titre :

Synthesis and antibacterial activity of new peptides from Alfalfa RuBisCO protein hydrolysates and mode of action via a membrane damage mechanism against Listeria innocua

Auteur(s) :

Kobbi, Sabrine [Auteur]
Nedjar, Naima [Auteur]
Institut Charles Viollette (ICV) - EA 7394 [ICV]
Chihib, Nour-Eddine [Auteur]
Unité Matériaux et Transformations (UMET) - UMR 8207
Balti, Rafik [Auteur]
Université de Sfax
Chevalier, Mickael [Auteur]
Institut Charles Viollette (ICV) - ULR 7394
Silvain, Amandine [Auteur]
Chaabouni, Semia [Auteur]
Université de Sfax
Dhulster, Pascal [Auteur]
Institut Charles Viollette (ICV) - ULR 7394
Bougatef, Ali [Auteur]
Université de Sfax

Titre de la revue :

Microbial Pathogenesis

Numéro :

115

Pagination :

41-49

Éditeur :

Elsevier BV

Date de publication :

2018-02

ISSN :

0882-4010

Discipline(s) HAL :

Sciences du Vivant [q-bio]/Ingénierie des aliments

Résumé en anglais : [en]

In this work we evaluated the mode of action of six new synthesized peptides (Met-Asp-Asn; Glu-leu-Ala-Ala-Ala-Cys; Leu-Arg-Asp-Asp-Phe; Gly-Asn-Ala-Pro-Gly-Ala-Val-Ala; Ala-Leu-Arg-Met-Ser-Gly and Arg-Asp-Arg-Phe–Leu), ...
Lire la suite >In this work we evaluated the mode of action of six new synthesized peptides (Met-Asp-Asn; Glu-leu-Ala-Ala-Ala-Cys; Leu-Arg-Asp-Asp-Phe; Gly-Asn-Ala-Pro-Gly-Ala-Val-Ala; Ala-Leu-Arg-Met-Ser-Gly and Arg-Asp-Arg-Phe–Leu), previously identified, from the most active peptide fractions of RuBisCO peptic hydrolysate against Listeria innocua via a membrane damage mechanism. Antibacterial effect and the minimum inhibitory concentrations (MIC) of these peptides were evaluated against six strains and their hemolytic activities towards bovine erythrocytes were determined. Prediction of the secondary structure of peptides indicated that these new antibacterial peptides are characterized by a short peptide chains (3–8 amino acid) and a random coli structure. Moreover, it was observed that one key characteristic of antibacterial peptides is the presence of specific amino acids such as cysteine, glycine, arginine and aspartic acid. In addition the determination of the extracellular potassium concentration revealed that treatment with pure RuBisCO peptides could cause morphological changes of L. innocua and destruction of the cell integrity via irreversible membrane damage. The results could provide information for investigating the antibacterial model of antibacterial peptides derived from RuBisCO protein hydrolysates.Lire moins >

Langue :

Anglais

Audience :

Internationale

Vulgarisation :

Non

Établissement(s) :

Université de Lille
CNRS
INRAE
ENSCL

Collections :

• BioEcoAgro - UMR-T 1158
• Unité Matériaux et Transformations (UMET) - UMR 8207

Équipe(s) de recherche :

Processus aux Interfaces et Hygiène des Matériaux (PIHM)

Date de dépôt :

2024-03-26T16:54:02Z