Vectorization by nanoparticles decreases ...
Type de document :
Article dans une revue scientifique: Article original
PMID :
URL permanente :
Titre :
Vectorization by nanoparticles decreases the overall toxicity of airborne pollutants
Auteur(s) :
Carpentier, Rodolphe [Auteur]
498252|||Lille Inflammation Research International Center - U 995 [LIRIC] (OLD)
Platel, Anne [Auteur]
IMPact de l'Environnement Chimique sur la Santé humaine (IMPECS) - ULR 4483
Institut Pasteur de Lille
Maiz-Gregores, Helena [Auteur]
Lille Inflammation Research International Center - U 995 [LIRIC]
Nesslany, Fabrice [Auteur]
IMPact de l'Environnement Chimique sur la Santé humaine (IMPECS) - ULR 4483
Institut Pasteur de Lille
Betbeder, Didier [Auteur]
Université d'Artois [UA]
498252|||Lille Inflammation Research International Center - U 995 [LIRIC] (OLD)
498252|||Lille Inflammation Research International Center - U 995 [LIRIC] (OLD)
Platel, Anne [Auteur]
IMPact de l'Environnement Chimique sur la Santé humaine (IMPECS) - ULR 4483
Institut Pasteur de Lille
Maiz-Gregores, Helena [Auteur]
Lille Inflammation Research International Center - U 995 [LIRIC]
Nesslany, Fabrice [Auteur]
IMPact de l'Environnement Chimique sur la Santé humaine (IMPECS) - ULR 4483
Institut Pasteur de Lille
Betbeder, Didier [Auteur]
Université d'Artois [UA]
498252|||Lille Inflammation Research International Center - U 995 [LIRIC] (OLD)
Titre de la revue :
PLoS ONE
Numéro :
12
Pagination :
e0183243
Éditeur :
Public Library of Science
Date de publication :
2017-08-15
ISSN :
1932-6203
Discipline(s) HAL :
Sciences du Vivant [q-bio]
Résumé en anglais : [en]
Atmospheric pollution is mainly composed of volatile pollutants and particulate matter that strongly interact. However, their specific roles in the induction of cellular toxicity, in particular the impact of the vectorization ...
Lire la suite >Atmospheric pollution is mainly composed of volatile pollutants and particulate matter that strongly interact. However, their specific roles in the induction of cellular toxicity, in particular the impact of the vectorization of atmospheric pollutants by ultrafine particles, remains to be fully elucidated. For this purpose, non-toxic poly-lactic co-glycolic acid (PLGA) nanoparticles were synthesized and three pollutants (benzo(a)pyrene, naphthalene and di-ethyl-hexyl-phthalate) were adsorbed on the surface of the nanoparticles in order to evaluate the toxicity (cytotoxicity, genotoxicity and ROS induction) of these complexes to a human airway epithelial cell line. The adsorption of the pollutants onto the nanoparticles was confirmed by HPLC analysis. Interestingly, the cytotoxicity assays (MTT, LDH and CellTox Green) clearly demonstrated that the vectorization by nanoparticles decreases the toxicity of the adsorbed pollutants. Genotoxicity was assessed by the micronucleus test and the comet assay and showed no increase in primary DNA damage or in chromosomal aberrations of nanoparticle vectorized pollutants. Neither cytotoxicity nor genotoxicity was correlated with ROS induction. To conclude, our results indicate that the vectorization of pollutants by nanoparticles does not potentiate the toxicity of the pollutants studied and that, on the contrary, adsorption onto nanoparticles could protect cells against pollutants' toxicity.Lire moins >
Lire la suite >Atmospheric pollution is mainly composed of volatile pollutants and particulate matter that strongly interact. However, their specific roles in the induction of cellular toxicity, in particular the impact of the vectorization of atmospheric pollutants by ultrafine particles, remains to be fully elucidated. For this purpose, non-toxic poly-lactic co-glycolic acid (PLGA) nanoparticles were synthesized and three pollutants (benzo(a)pyrene, naphthalene and di-ethyl-hexyl-phthalate) were adsorbed on the surface of the nanoparticles in order to evaluate the toxicity (cytotoxicity, genotoxicity and ROS induction) of these complexes to a human airway epithelial cell line. The adsorption of the pollutants onto the nanoparticles was confirmed by HPLC analysis. Interestingly, the cytotoxicity assays (MTT, LDH and CellTox Green) clearly demonstrated that the vectorization by nanoparticles decreases the toxicity of the adsorbed pollutants. Genotoxicity was assessed by the micronucleus test and the comet assay and showed no increase in primary DNA damage or in chromosomal aberrations of nanoparticle vectorized pollutants. Neither cytotoxicity nor genotoxicity was correlated with ROS induction. To conclude, our results indicate that the vectorization of pollutants by nanoparticles does not potentiate the toxicity of the pollutants studied and that, on the contrary, adsorption onto nanoparticles could protect cells against pollutants' toxicity.Lire moins >
Langue :
Anglais
Comité de lecture :
Oui
Audience :
Internationale
Vulgarisation :
Non
Autre(s) projet(s) ou source(s) de financement :
Conseil régional Nord – Pas de Calais
Conseil Régional des Hauts de France
Conseil Régional des Hauts de France
Établissement(s) :
CHU Lille
Inserm
Université de Lille
Institut Pasteur de Lille
Inserm
Université de Lille
Institut Pasteur de Lille
Équipe(s) de recherche :
Therapeutic innovation targetting inflammation
Date de dépôt :
2019-05-17T13:08:44Z
2022-09-07T10:01:19Z
2024-02-20T11:00:51Z
2022-09-07T10:01:19Z
2024-02-20T11:00:51Z
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- Carpentier et al.pdf
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