Mechanisms allowing protein delivery in nasal mucosa using NPL nanoparticles

Bernocchi, Beatrice; Carpentier, Rodolphe; Lantier, Isabelle; Ducournau, Celine; Dimier-Poisson, Isabelle; Betbeder, Didier

Document type :

Article dans une revue scientifique

DOI :

10.1016/j.jconrel.2016.04.014

PMID :

27080572

Permalink :

http://hdl.handle.net/20.500.12210/11276

Title :

Mechanisms allowing protein delivery in nasal mucosa using NPL nanoparticles

Author(s) :

Bernocchi, Beatrice [Auteur]
Carpentier, Rodolphe [Auteur]

Lille Inflammation Research International Center - U 995 [LIRIC]
Lille Inflammation Research International Center - U 995 [LIRIC]
Lantier, Isabelle [Auteur]
Ducournau, Celine [Auteur]
Dimier-Poisson, Isabelle [Auteur]
Betbeder, Didier [Auteur]

Lille Inflammation Research International Center - U 995 [LIRIC]
Lille Inflammation Research International Center - U 995 [LIRIC]

Journal title :

Journal of controlled release . official journal of the Controlled Release Society

Abbreviated title :

J. Control. Release

Volume number :

232

Pages :

42-50

Publication date :

2016-06-28

ISSN :

0168-3659

English keyword(s) :

Vaccine
Biodistribution
Transcytosis
Protein delivery
Intranasal drug delivery
Nanoparticle

HAL domain(s) :

Sciences du Vivant [q-bio]

English abstract : [en]

The intranasal administration of proteins using nanoparticles is a promising approach for several applications, especially for mucosal vaccines. Delivery of protein within the epithelial barrier is a key point to elicit ...
Show more >The intranasal administration of proteins using nanoparticles is a promising approach for several applications, especially for mucosal vaccines. Delivery of protein within the epithelial barrier is a key point to elicit an immune response and nano-carrier has to show no toxicity. The aim of this work was to elucidate the interactions of cationic porous nanoparticles loaded with protein delivery for antigen delivery in the nose. We investigated the loading, the cellular delivery and the epithelial transcytosis of proteins associated to these nanoparticles containing an anionic lipid in their core (NPL). NPL were highly endocytosed by airway epithelial cells and significantly improved the protein delivery into the cell. In vitro transcytosis studies showed that NPL did not modify the in vitro epithelial permeability suggesting no toxicity of these carriers. Moreover protein and NPL did not translocate the epithelial barrier. In vivo studies demonstrated that NPL prolonged the nasal residence time of the protein and no NPL were found beyond the epithelial barrier in vivo, precluding a negative side effect. All together these results establish the NPL as a bio-eliminable and optimal vaccine carrier.Show less >

Audience :

Internationale

Popular science :

Non

Administrative institution(s) :

CHU Lille
Inserm
Université de Lille

Collections :

Institut de Recherche Translationnelle sur l'Inflammation (INFINITE) - U1286

Research team(s) :

Therapeutic innovation targetting inflammation

Submission date :

2019-05-17T13:14:39Z

Mechanisms allowing protein delivery in ... BibTeX CSV Excel RIS

Mechanisms allowing protein delivery in ...

BibTeX

CSV

Excel

RIS