Title :

Ionizing radiation results in a mixture of cellular outcomes including mitotic catastrophe, senescence, methuosis, and iron-dependent cell death

Author(s) :

Adjemian, Sandy [Auteur]
Oltean, Teodora [Auteur]
Martens, Sofie [Auteur]
Wiernicki, Bartosz [Auteur]
Goossens, Vera [Auteur]
Vanden Berghe, Tom [Auteur]
Cappe, Benjamin [Auteur]
Ladik, Maria [Auteur]
Riquet, Franck B. [Auteur]
Laboratoire de Physique des Lasers, Atomes et Molécules - UMR 8523 [PhLAM]
Heyndrickx, Liesbeth [Auteur]
Bridelance, Jolien [Auteur]
Vuylsteke, Marnik [Auteur]
Vandecasteele, Katrien [Auteur]
Vandenabeele, Peter [Auteur]

Journal title :

CELL DEATH & DISEASE

Publication date :

2020

HAL domain(s) :

Physique [physics]/Physique [physics]/Biophysique [physics.bio-ph]

English abstract : [en]

Radiotherapy is commonly used as a cytotoxic treatment of a wide variety of tumors. Interestingly, few case reports underlined its potential to induce immune-mediated abscopal effects, resulting in regression of metastases, ...
Show more >Radiotherapy is commonly used as a cytotoxic treatment of a wide variety of tumors. Interestingly, few case reports underlined its potential to induce immune-mediated abscopal effects, resulting in regression of metastases, distant from the irradiated site. These observations are rare, and apparently depend on the dose used, suggesting that dose-related cellular responses may be involved in the distant immunogenic responses. Ionizing radiation (IR) has been reported to elicit immunogenic apoptosis, necroptosis, mitotic catastrophe, and senescence. In order to link a cellular outcome with a particular dose of irradiation, we performed a systematic study in a panel of cell lines on the cellular responses at different doses of X-rays. Remarkably, we observed that all cell lines tested responded in a similar fashion to IR with characteristics of mitotic catastrophe, senescence, lipid peroxidation, and caspase activity. Iron chelators (but not Ferrostatin-1 or vitamin E) could prevent the formation of lipid peroxides and cell death induced by IR, suggesting a crucial role of iron-dependent cell death during high-dose irradiation. We also show that in K-Ras-mutated cells, IR can induce morphological features reminiscent of methuosis, a cell death modality that has been recently described following H-Ras or K-Ras mutation overexpression.Show less >

Language :

Anglais

Collections :

• Laboratoire de Physique des Lasers, Atomes et Molécules (PhLAM) - UMR 8523

Source :

Harvested from HAL