Title :

Cell surface of Lactococcus lactis is covered by a protective polysaccharide pellicle.

Author(s) :

Chapot-Chartier, Marie-Pierre [Auteur]
MICrobiologie de l'ALImentation au Service de la Santé [MICALIS]
Vinogradov, Evgeny [Auteur]
National Research Council of Canada [NRC]
Sadovskaya, Irina [Auteur]
Laboratoire de Recherche sur les Biomatériaux et les Biotechnologies [LR2B]
Andre, Guillaume [Auteur]
Mistou, Michel-Yves [Auteur]
MICrobiologie de l'ALImentation au Service de la Santé [MICALIS]
Trieu-Cuot, Patrick [Auteur]
Biologie des Bactéries Pathogènes à Gram-positif
Furlan, Sylviane [Auteur]
MICrobiologie de l'ALImentation au Service de la Santé [MICALIS]
Bidnenko, Elena [Auteur]
MICrobiologie de l'ALImentation au Service de la Santé [MICALIS]
Courtin, Pascal [Auteur]
MICrobiologie de l'ALImentation au Service de la Santé [MICALIS]
Péchoux, Christine [Auteur]
Unité de recherche génomique et physiologie de la lactation [GPL]
Hols, Pascal [Auteur]
Dufrêne, Yves F [Auteur]
Kulakauskas, Saulius [Auteur]
MICrobiologie de l'ALImentation au Service de la Santé [MICALIS]

Journal title :

Journal of Biological Chemistry

Pages :

10464-71

Publisher :

American Society for Biochemistry and Molecular Biology

Publication date :

2010-04-02

ISSN :

0021-9258

English keyword(s) :

POLYSACCHARIDE
LACTOCOCCUS LACTIS
COMPLETE GENOME
GRAM-POSITIVE BACTERIA
ATOMIC-FORCE MICROSCOPY

HAL domain(s) :

Sciences du Vivant [q-bio]/Biochimie, Biologie Moléculaire/Biochimie [q-bio.BM]
Sciences du Vivant [q-bio]/Microbiologie et Parasitologie/Bactériologie

English abstract : [en]

In Gram-positive bacteria, the functional role of surface polysaccharides (PS) that are not of capsular nature remains poorly understood. Here, we report the presence of a novel cell wall PS pellicle on the surface of ...
Show more >In Gram-positive bacteria, the functional role of surface polysaccharides (PS) that are not of capsular nature remains poorly understood. Here, we report the presence of a novel cell wall PS pellicle on the surface of Lactococcus lactis. Spontaneous PS-negative mutants were selected using semi-liquid growth conditions, and all mutations were mapped in a single chromosomal locus coding for PS biosynthesis. PS molecules were shown to be composed of hexasaccharide phosphate repeating units that are distinct from other bacterial PS. Using complementary atomic force and transmission electron microscopy techniques, we showed that the PS layer forms an outer pellicle surrounding the cell. Notably, we found that this cell wall layer confers a protective barrier against host phagocytosis by murine macrophages. Altogether, our results suggest that the PS pellicle could represent a new cell envelope structural component of Gram-positive bacteria.Show less >

Language :

Anglais

Popular science :

Non

Collections :

• Marrow Adiposity & Bone Lab (MABLab) - ULR 4490

Source :

Harvested from HAL