Title :

Red light induces starch accumulation in Chlorella vulgaris without affecting photosynthesis efficiency, unlike abiotic stress

Author(s) :

Six, A. [Auteur]
CEA Cadarache
Dimitriades-Lemaire, A. [Auteur]
CEA Cadarache
Lancelon-Pin, C. [Auteur]
Centre de Recherches sur les Macromolécules Végétales [CERMAV ]
Putaux, J.-L. [Auteur]
Centre de Recherches sur les Macromolécules Végétales [CERMAV]
Dauvillee, David [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576
Petroutsos, D. [Auteur]
Uppsala University
Alvarez Diaz, P. [Auteur]
CEA Cadarache
Sassi, J.-F. [Auteur]
CEA Cadarache
Li-Beisson, Y. [Auteur]
Institut de Biosciences et Biotechnologies d'Aix-Marseille (ex-IBEB) [BIAM]
Fleury, G. [Auteur]
CEA Cadarache

Journal title :

Algal Research

Abbreviated title :

Algal Research

Volume number :

80

Pages :

103515

Publisher :

Elsevier BV

Publication date :

2024-06

ISSN :

2211-9264

English keyword(s) :

Chlorella vulgaris
Starch
Light quality
Nitrogen deprivation
Supra-optimal temperature
Bioplastics

HAL domain(s) :

Sciences du Vivant [q-bio]

English abstract : [en]

Microalgae show great promise as sources of starch, one of the most widely consumed macromolecules. In this study, we evaluated the impact of three starch-inducing factors, namely nitrogen deprivation, supra-optimal ...
Show more >Microalgae show great promise as sources of starch, one of the most widely consumed macromolecules. In this study, we evaluated the impact of three starch-inducing factors, namely nitrogen deprivation, supra-optimal temperature, and red light, on the physiology and starch accumulation capacity of Chlorella vulgaris. This starch accumulation was monitored by measuring the total carbohydrate content and transmission electron microscopy (TEM) imaging. Nitrogen deprivation and a supra-optimal temperature of 39 °C resulted in carbohydrate contents of 69.7 and 64.3 % of dry weight (DW) respectively. This constituted a 5.3- and 3.3-fold increase in carbohydrate productivity compared to the control, after 4 days of cultivation. During this period, carbohydrates represented over 80 % of the produced material (DW basis). However, nitrogen deprivation and supra-optimal temperature were accompanied by extensive stress, leading to lower cell division rates and damage to the photosynthetic apparatus. Red light illumination resulted in a more moderate production of carbohydrates. After 4 days of cultivation, the carbohydrate content reached 46.8 %, representing a 3.0-fold increase in productivity compared to control. The composition of the starch formed under red light was surprisingly poor in amylose, similar to transitory-type starch rather than storage starch. Most notably, the starch accumulation under red light was sustained over 7 days without affecting the rate of cell division and quantum yield efficiency. To the best of our knowledge, red light is the only factor reported so far to induce a significant starch accumulation without hindering cell division and photosynthesis efficiency, even after long-term exposure (7 days). Furthermore, all three conditions induced a cell wall thickening, albeit without affecting the recovery of accumulated starch by high-pressure homogenization. These results highlight the potential of red light as a starch inducer in Chlorella vulgaris and open up perspectives for the production of starch-based bioplastics from microalgae.Show less >

Language :

Anglais

Audience :

Internationale

Popular science :

Non

Administrative institution(s) :

Université de Lille
CNRS

Collections :

• Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576

Research team(s) :

Plant Storage Polysaccharides

Submission date :

2024-05-07T09:27:31Z
2024-05-17T07:50:49Z