Title :

(n-3) long-chain PUFA differentially affect resistance to Pseudomonas aeruginosa infection of male and female cftr-/- mice.

Author(s) :

Tiesset, Hélène [Auteur]
Lille Inflammation Research International Center - U 995 [LIRIC]
Bernard, Henry [Auteur]
Lille Inflammation Research International Center - U 995 [LIRIC]
Bartke, Nana [Auteur]
Beermann, Christopher [Auteur]
Flachaire, Elisabeth [Auteur]
Desseyn, Jean-Luc [Auteur]
Lille Inflammation Research International Center - U 995 [LIRIC]
gottrand, frederic [Auteur]
Lille Inflammation Research International Center - U 995 [LIRIC]
Husson, Marie-Odile [Auteur]
Lille Inflammation Research International Center - U 995 [LIRIC]

Journal title :

The Journal of Nutrition

Abbreviated title :

J Nutr

Volume number :

141

Pages :

1101-7

Publication date :

2011-06-01

ISSN :

1541-6100

English keyword(s) :

Animals
Bronchoalveolar Lavage Fluid
Cystic Fibrosis
Cystic Fibrosis Transmembrane Conductance Regulator
Cytokines
Dietary Supplements
Disease Susceptibility
Fatty Acids
Omega-3
Female
Humans
Inflammation Mediators
Lung
Male
Mice
Mice
129 Strain
Mice
Inbred C57BL
Mice
Inbred CFTR
Mice
Knockout
Opportunistic Infections
Pneumonia
Bacterial
Pseudomonas Infections
Sex Factors

HAL domain(s) :

Sciences du Vivant [q-bio]

English abstract : [en]

The aim of this study was to determine whether oral supplementation with EPA/DHA (10.5 and 5.1% of fat, respectively) could improve the outcome of pulmonary P. aeruginosa infection in cftr(-/-) mice compared with wild-type ...
Show more >The aim of this study was to determine whether oral supplementation with EPA/DHA (10.5 and 5.1% of fat, respectively) could improve the outcome of pulmonary P. aeruginosa infection in cftr(-/-) mice compared with wild-type (Wt) mice similarly treated. Because gender could influence the susceptibility of cftr-deficient mice, results were analyzed by gender. Wt and (-/-) mice were randomized for 6 wk to consume a control or EPA/DHA diet, infected with endotracheal injection of 5 × 10(7) CFU/mouse of P. aeruginosa, and killed 24 h later. Cftr(-/-) mice were more susceptible to infection than were Wt mice; (-/-) males had more neutrophils (P < 0.01) and a higher keratinocyte-derived chemokine (KC) level (P < 0.05), and (-/-) females had greater lung injury and mortality (P < 0.05). Female (-/-) mice were more susceptible than (-/-) males with a higher mortality and lung injury (P < 0.05). The EPA/DHA diet reduced neutrophil numbers and KC and IL-6 levels (P < 0.05) in (-/-) males and reduced mortality rate (P < 0.001), lung permeability, and IL-6 level (P < 0.05) in (-/-) females compared with (-/-) mice fed the control diet. These results were associated with a reduction in the pulmonary bacterial load (P < 0.05), an increase in the EPA/DHA concentration in cell membranes of (-/-) males and females (P < 0.01), and an increased weight gain only in males compared with (-/-) mice fed the control diet (P < 0.01). In conclusion, EPA/DHA improves the host resistance of (-/-) mice, although the beneficial effect differed in males and females.Show less >

Language :

Anglais

Audience :

Non spécifiée

Collections :

• Institut de Recherche Translationnelle sur l'Inflammation (INFINITE) - U1286

Submission date :

2019-07-09T14:01:21Z
2019-07-10T09:11:21Z