Title :

Omega-3 polyunsaturated fatty acids improve host response in chronic Pseudomonas aeruginosa lung infection in mice.

Author(s) :

Pierre, Maud [Auteur]
Lille Inflammation Research International Center - U 995 [LIRIC]
Le Berre, Rozenn [Auteur]
Faculté de Médecine Henri Warembourg - Université de Lille
Desseyn, Jean-Luc [Auteur]
Lille Inflammation Research International Center - U 995 [LIRIC]
Galabert, Claude [Auteur]
Hôpital Renée Sabran [CHU - HCL]
Beghin, Laurent [Auteur]
Lille Inflammation Research International Center - U 995 [LIRIC]
Beermann, Christopher [Auteur]
University of Applied Sciences [Fulda]
Dagenais, André [Auteur]
Centre Hospitalier de l'Université de Montréal [CHUM]
Berthiaume, Yves [Auteur]
Centre Hospitalier de l'Université de Montréal [CHUM]
cardinaud, bruno [Auteur]
Université Nice Sophia Antipolis (1965 - 2019) [UNS]
barbry, pascal [Auteur]
Université Nice Sophia Antipolis (1965 - 2019) [UNS]
gottrand, Fréderic [Auteur]
Lille Inflammation Research International Center - U 995 [LIRIC]
guery, benoit [Auteur]
Faculté de Médecine Henri Warembourg - Université de Lille
cardinaud, bruno [Auteur]

Journal title :

American journal of physiology. Lung cellular and molecular physiology

Abbreviated title :

Am J Physiol Lung Cell Mol Physiol

Volume number :

292

Pages :

L1422-31

Publication date :

2007-06-01

ISSN :

1040-0605

English keyword(s) :

Animal Feed
Animals
Bronchoalveolar Lavage Fluid
Epithelial Sodium Channels
Extravascular Lung Water
Fatty Acids
Omega-3
Interleukin-6
Male
Mice
Mice
Inbred C57BL
Neutrophils
Pneumonia
Bacterial
Pseudomonas Infections
Pseudomonas aeruginosa
Pulmonary Alveoli
RNA
Messenger
Sodium-Potassium-Exchanging ATPase
Tumor Necrosis Factor-alpha
Weight Loss

HAL domain(s) :

Sciences du Vivant [q-bio]

English abstract : [en]

Pseudomonas aeruginosa is a gram-negative bacilli frequently encountered in human pathology. This pathogen is involved in a large number of nosocomial infections and chronic diseases. Herein we investigated the effects of ...
Show more >Pseudomonas aeruginosa is a gram-negative bacilli frequently encountered in human pathology. This pathogen is involved in a large number of nosocomial infections and chronic diseases. Herein we investigated the effects of polyunsaturated fatty acids (PUFA) in chronic Pseudomonas aeruginosa lung infection. C57BL/6 mice were fed for 5 wk with specifically designed diets with high contents in either omega-3 (omega-3) or omega-6 PUFA and compared to a control diet. P. aeruginosa included in agarose beads was then instilled intratracheally, and the animals were studied for 7 days. On the 4th day, the mice fed with the omega-3 diet had a higher lean body mass gain and a lower omega-6:omega-3 ratio of fatty acids extracted from the lung tissue compared with the other groups (P < 0.05). The omega-3 group had the lowest mortality. Distal alveolar fluid clearance (DAFC) as well as the inflammatory response and the cellular recruitment were higher in the omega-3 group on the 4th day. The effect on DAFC was independent of alpha-epithelial Na(+) channels (alpha-ENaC), beta-ENaC, and alpha(1)-Na-K-ATPase mRNA expressions, which were not altered by the different diets. In conclusion, a diet enriched in omega-3 PUFA can change lung membrane composition and improve survival in chronic pneumonia. This effect on survival is probably multifactorial involving the increased DAFC capacity as well as the optimization of the initial inflammatory response. This work suggests that a better control of the omega-6/omega-3 PUFA balance may represent an interesting target in the prevention and/or control of P. aeruginosa infection in patients.Show less >

Language :

Anglais

Audience :

Non spécifiée

Collections :

• Institut de Recherche Translationnelle sur l'Inflammation (INFINITE) - U1286

Submission date :

2019-07-09T14:03:11Z
2019-07-10T09:26:02Z
2021-05-28T07:55:37Z
2021-11-10T09:54:43Z
2022-02-02T10:05:30Z