Title :

Systematic gene overexpression in Candida albicans identifies a regulator of early adaptation to the mammalian gut

Author(s) :

Znaidi, Sadri [Auteur]
Biologie et Pathogénicité fongiques - Fungal Biology and Pathogenicity [BPF]
Van Wijlick, Lasse [Auteur]
Biologie et Pathogénicité fongiques - Fungal Biology and Pathogenicity [BPF]
Hernández-Cervantes, Arturo [Auteur]
Biologie et Pathogénicité fongiques - Fungal Biology and Pathogenicity [BPF]
Sertour, Natacha [Auteur]
Biologie et Pathogénicité fongiques - Fungal Biology and Pathogenicity [BPF]
Desseyn, Jean-Luc [Auteur]
Lille Inflammation Research International Center - U 995 [LIRIC]
Vincent, Frédéric [Auteur]
Université de Bourgogne [UB]
Atanassova, Ralitsa [Auteur]
Université de Bourgogne [UB]
Gouyer, Valérie [Auteur]
Lille Inflammation Research International Center - U 995 [LIRIC]
Munro, Carol A [Auteur]
Bachellier-Bassi, Sophie [Auteur]
Biologie et Pathogénicité fongiques - Fungal Biology and Pathogenicity [BPF]
Dalle, Frédéric [Auteur]
Université de Bourgogne [UB]
Bougnoux, Marie-Elisabeth [Auteur]
Biologie et Pathogénicité fongiques - Fungal Biology and Pathogenicity [BPF]
D'enfert, Christophe [Auteur]
Biologie et Pathogénicité fongiques - Fungal Biology and Pathogenicity [BPF]

Journal title :

Cellular Microbiology

Abbreviated title :

Cell Microbiol

Volume number :

20

Pages :

e12890

Publication date :

2018-11

ISSN :

1462-5822

English keyword(s) :

CRZ2
Candida albicans
chromatin immunoprecipitation-on-chip
gastrointestinal colonisation
regulatory networks
signature-tagged overexpression
transcriptomics

HAL domain(s) :

Sciences du Vivant [q-bio]

English abstract : [en]

Candida albicans is part of the human gastrointestinal (GI) microbiota. To better understand how C. albicans efficiently establishes GI colonisation, we competitively challenged growth of 572 signature-tagged strains (~10% ...
Show more >Candida albicans is part of the human gastrointestinal (GI) microbiota. To better understand how C. albicans efficiently establishes GI colonisation, we competitively challenged growth of 572 signature-tagged strains (~10% genome coverage), each conditionally overexpressing a single gene, in the murine gut. We identified CRZ2, a transcription factor whose overexpression and deletion respectively increased and decreased early GI colonisation. Using clues from genome-wide expression and gene-set enrichment analyses, we found that the optimal activity of Crz2p occurs under hypoxia at 37°C, as evidenced by both phenotypic and transcriptomic analyses following CRZ2 genetic perturbation. Consistent with early colonisation of the GI tract, we show that CRZ2 overexpression confers resistance to acidic pH and bile salts, suggesting an adaptation to the upper sections of the gut. Genome-wide location analyses revealed that Crz2p directly modulates the expression of many mannosyltransferase- and cell-wall protein-encoding genes, suggesting a link with cell-wall function. We show that CRZ2 overexpression alters cell-wall phosphomannan abundance and increases sensitivity to tunicamycin, suggesting a role in protein glycosylation. Our study reflects the powerful use of gene overexpression as a complementary approach to gene deletion to identify relevant biological pathways involved in C. albicans interaction with the host environment.Show less >

Language :

Anglais

Audience :

Non spécifiée

Popular science :

Non

Collections :

• Institut de Recherche Translationnelle sur l'Inflammation (INFINITE) - U1286

Submission date :

2019-07-10T08:45:03Z
2019-07-10T09:33:20Z
2024-03-20T12:17:29Z
2024-03-20T12:20:50Z
2024-03-20T12:27:48Z
2024-03-20T12:29:01Z