Transcription factor FOXD1 and miRNA-204-5p ...
Document type :
Article dans une revue scientifique
Permalink :
Title :
Transcription factor FOXD1 and miRNA-204-5p play a major role in B4GALNT2 downregulation in colon cancer
Author(s) :
Duca, Martina [Auteur]
Alma Mater Studiorum Università di Bologna = University of Bologna [UNIBO]
Malagolini, Nadia [Auteur]
Alma Mater Studiorum Università di Bologna = University of Bologna [UNIBO]
Pucci, Michela [Auteur]
Alma Mater Studiorum Università di Bologna = University of Bologna [UNIBO]
Cogez, Virginie [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576
Harduin-Lepers, Anne [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576
Dall’Olio, Fabio [Auteur]
Alma Mater Studiorum Università di Bologna = University of Bologna [UNIBO]
Alma Mater Studiorum Università di Bologna = University of Bologna [UNIBO]
Malagolini, Nadia [Auteur]
Alma Mater Studiorum Università di Bologna = University of Bologna [UNIBO]
Pucci, Michela [Auteur]
Alma Mater Studiorum Università di Bologna = University of Bologna [UNIBO]
Cogez, Virginie [Auteur]

Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576
Harduin-Lepers, Anne [Auteur]

Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576
Dall’Olio, Fabio [Auteur]
Alma Mater Studiorum Università di Bologna = University of Bologna [UNIBO]
Journal title :
Scientific Reports
Abbreviated title :
Sci Rep
Volume number :
15
Pages :
1821
Publisher :
Nature Publishing Group
Publication date :
2025-01-13
ISSN :
2045-2322
English keyword(s) :
Glycosylation
Sda antigen
Gene regulation
FOXD1
miR-204-5p
Colorectal cancer
Sda antigen
Gene regulation
FOXD1
miR-204-5p
Colorectal cancer
HAL domain(s) :
Sciences du Vivant [q-bio]
Chimie/Chimie théorique et/ou physique
Chimie/Chimie théorique et/ou physique
English abstract : [en]
The β1,4-N-acetylgalactosaminyltransferase 2 (B4GALNT2) which synthesizes the histo-blood group antigen Sda is highly expressed by normal colon, but it is dramatically down-regulated in colorectal cancer (CRC). High B4GALNT2 ...
Show more >The β1,4-N-acetylgalactosaminyltransferase 2 (B4GALNT2) which synthesizes the histo-blood group antigen Sda is highly expressed by normal colon, but it is dramatically down-regulated in colorectal cancer (CRC). High B4GALNT2 expression in CRC tissues is a marker of longer survival. The molecular bases of B4GALNT2 inhibition in CRC are largely obscure. A key role may be played by transcription factors and miRNA. Through an in silico analysis of The Cancer Genome Atlas and of the Cancer Cell Line Encyclopedia, we identified the transcription factors FOXD1, FOXF2 and PGR as well as mir-204-5p as potential inhibitory agents. Their transient transfection in the cell line GP2d, whose B4GALNT2 is closer to that of a normal mucosa, confirmed their inhibitory activity with a crucial role for FOXD1. The latter inhibited B4GALNT2 also in the middle B4GALNT2 expresser cell line Caco2. Deletion experiments of the putative FOXD1 binding sites in the ~ 2800 bp sequence upstream of the B4GALNT2 transcriptional start site cloned in frame with the luciferase reporter gene, confirmed the regulatory role of FOXD1. Finally, FOXD1 knock down in the non-B4GALNT2 expresser cell line SW948 stimulated B4GALNT2. Thus, FOXD1 and miR-204-5p emerged as crucial new player of B4GALNT2 down-regulation in CRC.Show less >
Show more >The β1,4-N-acetylgalactosaminyltransferase 2 (B4GALNT2) which synthesizes the histo-blood group antigen Sda is highly expressed by normal colon, but it is dramatically down-regulated in colorectal cancer (CRC). High B4GALNT2 expression in CRC tissues is a marker of longer survival. The molecular bases of B4GALNT2 inhibition in CRC are largely obscure. A key role may be played by transcription factors and miRNA. Through an in silico analysis of The Cancer Genome Atlas and of the Cancer Cell Line Encyclopedia, we identified the transcription factors FOXD1, FOXF2 and PGR as well as mir-204-5p as potential inhibitory agents. Their transient transfection in the cell line GP2d, whose B4GALNT2 is closer to that of a normal mucosa, confirmed their inhibitory activity with a crucial role for FOXD1. The latter inhibited B4GALNT2 also in the middle B4GALNT2 expresser cell line Caco2. Deletion experiments of the putative FOXD1 binding sites in the ~ 2800 bp sequence upstream of the B4GALNT2 transcriptional start site cloned in frame with the luciferase reporter gene, confirmed the regulatory role of FOXD1. Finally, FOXD1 knock down in the non-B4GALNT2 expresser cell line SW948 stimulated B4GALNT2. Thus, FOXD1 and miR-204-5p emerged as crucial new player of B4GALNT2 down-regulation in CRC.Show less >
Language :
Anglais
Peer reviewed article :
Oui
Audience :
Internationale
Popular science :
Non
Administrative institution(s) :
Université de Lille
CNRS
CNRS
Research team(s) :
Régulation de la glycosylation terminale
Submission date :
2025-01-20T10:39:20Z
2025-01-22T14:02:28Z
2025-01-22T14:27:55Z
2025-01-22T14:02:28Z
2025-01-22T14:27:55Z
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