Penta-block copolymer microspheres: Impact ...
Document type :
Article dans une revue scientifique: Article original
PMID :
Permalink :
Title :
Penta-block copolymer microspheres: Impact of polymer characteristics and process parameters on protein release
Author(s) :
Le, Minh-Quan [Auteur]
Violet, Fabien [Auteur]
Paniagua, Cedric [Auteur]
Garric, Xavier [Auteur]
Venier-Julienne, Marie-Claire [Auteur]
Violet, Fabien [Auteur]
Paniagua, Cedric [Auteur]
Garric, Xavier [Auteur]
Venier-Julienne, Marie-Claire [Auteur]
Journal title :
International Journal of Pharmaceutics
Abbreviated title :
Int. J. Pharm.
Volume number :
535
Pages :
428-437
Publication date :
2018-01-15
ISSN :
0378-5173
English keyword(s) :
Protein sustained release
Morphology
Penta-block copolymer
Microencapsulation
Morphology
Penta-block copolymer
Microencapsulation
HAL domain(s) :
Sciences du Vivant [q-bio]
English abstract : [en]
Here, we aimed to develop protein loaded microspheres (MSs) using penta-block PLGA-based copolymers to obtain sustained and complete protein release. We varied MS morphology and studied the control of protein release. ...
Show more >Here, we aimed to develop protein loaded microspheres (MSs) using penta-block PLGA-based copolymers to obtain sustained and complete protein release. We varied MS morphology and studied the control of protein release. Lysozyme was used as a model protein and MSs were prepared using the solid-in-oil-in-water emulsion solvent extraction method. We synthesized and studied various penta-block PLGA-based copolymers. Copolymer characteristics (LA/GA ratio and molecular weight of PLGA blocks) influenced MS morphology. MS porosity was influenced by process parameters (such as solvent type, polymer concentration, emulsifying speed), whereas the aqueous volume for extraction and stabilizer did not have a significant effect. MSs of the same size, but different morphologies, exhibited different protein release behavior, with porous structures being essential for the continuous and complete release of encapsulated protein. These findings suggest strategies to engineer the morphology of MSs produced from PLGA-based multi-block copolymers to achieve appropriate release rates for a protein delivery system.Show less >
Show more >Here, we aimed to develop protein loaded microspheres (MSs) using penta-block PLGA-based copolymers to obtain sustained and complete protein release. We varied MS morphology and studied the control of protein release. Lysozyme was used as a model protein and MSs were prepared using the solid-in-oil-in-water emulsion solvent extraction method. We synthesized and studied various penta-block PLGA-based copolymers. Copolymer characteristics (LA/GA ratio and molecular weight of PLGA blocks) influenced MS morphology. MS porosity was influenced by process parameters (such as solvent type, polymer concentration, emulsifying speed), whereas the aqueous volume for extraction and stabilizer did not have a significant effect. MSs of the same size, but different morphologies, exhibited different protein release behavior, with porous structures being essential for the continuous and complete release of encapsulated protein. These findings suggest strategies to engineer the morphology of MSs produced from PLGA-based multi-block copolymers to achieve appropriate release rates for a protein delivery system.Show less >
Language :
Anglais
Audience :
Internationale
Popular science :
Non
Submission date :
2019-10-22T08:09:26Z