Title :

Penta-block copolymer microspheres: Impact of polymer characteristics and process parameters on protein release

Author(s) :

Le, Minh-Quan [Auteur]
Violet, Fabien [Auteur]
Paniagua, Cedric [Auteur]
Garric, Xavier [Auteur]
Venier-Julienne, Marie-Claire [Auteur]

Journal title :

International Journal of Pharmaceutics

Abbreviated title :

Int. J. Pharm.

Volume number :

535

Pages :

428-437

Publication date :

2018-01-15

ISSN :

0378-5173

English keyword(s) :

Protein sustained release
Morphology
Penta-block copolymer
Microencapsulation

HAL domain(s) :

Sciences du Vivant [q-bio]

English abstract : [en]

Here, we aimed to develop protein loaded microspheres (MSs) using penta-block PLGA-based copolymers to obtain sustained and complete protein release. We varied MS morphology and studied the control of protein release. ...
Show more >Here, we aimed to develop protein loaded microspheres (MSs) using penta-block PLGA-based copolymers to obtain sustained and complete protein release. We varied MS morphology and studied the control of protein release. Lysozyme was used as a model protein and MSs were prepared using the solid-in-oil-in-water emulsion solvent extraction method. We synthesized and studied various penta-block PLGA-based copolymers. Copolymer characteristics (LA/GA ratio and molecular weight of PLGA blocks) influenced MS morphology. MS porosity was influenced by process parameters (such as solvent type, polymer concentration, emulsifying speed), whereas the aqueous volume for extraction and stabilizer did not have a significant effect. MSs of the same size, but different morphologies, exhibited different protein release behavior, with porous structures being essential for the continuous and complete release of encapsulated protein. These findings suggest strategies to engineer the morphology of MSs produced from PLGA-based multi-block copolymers to achieve appropriate release rates for a protein delivery system.Show less >

Language :

Anglais

Audience :

Internationale

Popular science :

Non

Collections :

• Institut de Recherche Translationnelle sur l'Inflammation (INFINITE) - U1286

Submission date :

2019-10-22T08:09:26Z