A decrease in anaerobic bacteria promotes ...
Document type :
Article dans une revue scientifique: Article original
PMID :
Permalink :
Title :
A decrease in anaerobic bacteria promotes Candida glabrata overgrowth while beta-glucan treatment restores the gut microbiota and attenuates colitis
Author(s) :
Charlet, Rogatien [Auteur]
Lille Inflammation Research International Center (LIRIC) - U995
Bortolus, Clovis [Auteur]
498252|||Lille Inflammation Research International Center - U 995 [LIRIC] (OLD)
Barbet, Melissandre [Auteur]
Lille Inflammation Research International Center - U 995 [LIRIC]
Sendid, Boualem [Auteur]
Lille Inflammation Research International Center (LIRIC) - U995
Jawhara, Samir [Auteur]
Lille Inflammation Research International Center - U 995 [LIRIC]
Lille Inflammation Research International Center (LIRIC) - U995
Bortolus, Clovis [Auteur]
498252|||Lille Inflammation Research International Center - U 995 [LIRIC] (OLD)
Barbet, Melissandre [Auteur]
Lille Inflammation Research International Center - U 995 [LIRIC]
Sendid, Boualem [Auteur]

Lille Inflammation Research International Center (LIRIC) - U995
Jawhara, Samir [Auteur]

Lille Inflammation Research International Center - U 995 [LIRIC]
Journal title :
Gut Pathogens
Abbreviated title :
Gut Pathogens
Volume number :
10
Publication date :
2018-12-03
ISSN :
1757-4749
English keyword(s) :
Microbiota
Anaerobic bacteria
Enterococcus faecalis
Escherichia coli
beta-Glucans
Candida glabrata
Colitis
Anaerobic bacteria
Enterococcus faecalis
Escherichia coli
beta-Glucans
Candida glabrata
Colitis
HAL domain(s) :
Sciences du Vivant [q-bio]
English abstract : [en]
BACKGROUND: The intestinal microbiota plays a crucial role in the maintenance of gut homeostasis. Changes in crosstalk between the intestinal epithelial cells, immune cells and the microbiota are critically involved in the ...
Show more >BACKGROUND: The intestinal microbiota plays a crucial role in the maintenance of gut homeostasis. Changes in crosstalk between the intestinal epithelial cells, immune cells and the microbiota are critically involved in the development of inflammatory bowel disease. In the experimental mouse model, the development of colitis induced by dextran sulfate sodium (DSS) promotes overgrowth of the opportunistic yeast pathogen . Conversely, fungal colonization aggravates inflammatory parameters. In the present study, we explored the effect of colonization on the diversity of the gut microbiota in a DSS-induced colitis model, and determined the impact of soluble β-glucans on -host interactions. RESULTS: Mice were administered a single inoculum of and were exposed to DSS treatment for 2 weeks in order to induce acute colitis. For β-glucan treatment, mice were administered with soluble β-glucans purified from (3 mg per mouse), orally and daily, for 5 days, starting on day 1. The number of colonies and changes in microbiota diversity were assessed in freshly collected stool samples from each tagged mouse, using traditional culture methods based on agar plates. An increase in and populations and a reduction in and were observed during colitis development. This decrease in was significantly accentuated by overgrowth. Oral administration of β-glucans to mice decreased the overgrowth of aerobic bacteria and IL-1β expression while and populations increased significantly. β-glucan treatment increased IL-10 production via PPARγ sensing, promoting the attenuation of colitis and elimination. CONCLUSIONS: This study shows that the colonic inflammation alters the microbial balance, while β-glucan treatment increases the anaerobic bacteria and promotes colitis attenuation and elimination.Show less >
Show more >BACKGROUND: The intestinal microbiota plays a crucial role in the maintenance of gut homeostasis. Changes in crosstalk between the intestinal epithelial cells, immune cells and the microbiota are critically involved in the development of inflammatory bowel disease. In the experimental mouse model, the development of colitis induced by dextran sulfate sodium (DSS) promotes overgrowth of the opportunistic yeast pathogen . Conversely, fungal colonization aggravates inflammatory parameters. In the present study, we explored the effect of colonization on the diversity of the gut microbiota in a DSS-induced colitis model, and determined the impact of soluble β-glucans on -host interactions. RESULTS: Mice were administered a single inoculum of and were exposed to DSS treatment for 2 weeks in order to induce acute colitis. For β-glucan treatment, mice were administered with soluble β-glucans purified from (3 mg per mouse), orally and daily, for 5 days, starting on day 1. The number of colonies and changes in microbiota diversity were assessed in freshly collected stool samples from each tagged mouse, using traditional culture methods based on agar plates. An increase in and populations and a reduction in and were observed during colitis development. This decrease in was significantly accentuated by overgrowth. Oral administration of β-glucans to mice decreased the overgrowth of aerobic bacteria and IL-1β expression while and populations increased significantly. β-glucan treatment increased IL-10 production via PPARγ sensing, promoting the attenuation of colitis and elimination. CONCLUSIONS: This study shows that the colonic inflammation alters the microbial balance, while β-glucan treatment increases the anaerobic bacteria and promotes colitis attenuation and elimination.Show less >
Language :
Anglais
Audience :
Internationale
Popular science :
Non
Administrative institution(s) :
CHU Lille
Inserm
Université de Lille
Inserm
Université de Lille
Submission date :
2019-10-22T08:10:02Z
2024-03-05T11:23:41Z
2024-03-05T11:23:41Z
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