Title :

Separation of enantiomers of native amino acids with polysaccharide-based chiral columns in supercritical fluid chromatography.

Author(s) :

Lipka, Emmanuelle [Auteur]
Lille Inflammation Research International Center - U 995 [LIRIC]
Lille Inflammation Research International Center - U 995 [LIRIC]
Dascalu, Anca [Auteur]
Messara, Yasmine [Auteur]
Tsutsqiridze, Eka [Auteur]
Farkas, Tivadar [Auteur]
Chankvetadze, Bezhan [Auteur]

Journal title :

Journal of chromatography. A

Abbreviated title :

J Chromatogr A

Publication date :

2018-11-22

ISSN :

1873-3778

English keyword(s) :

Extra column band-broadening
Separation of enantiomers
Polysaccharide-based chiral stationary phases
Native amino-acids
Mobile phase composition

HAL domain(s) :

Sciences du Vivant [q-bio]

English abstract : [en]

In this short communication preliminary results are reported on the separation of enantiomers of underivatized amino acids on polysaccharide-based chiral columns in sub/super-critical fluid chromatography (SFC). Racemic ...
Show more >In this short communication preliminary results are reported on the separation of enantiomers of underivatized amino acids on polysaccharide-based chiral columns in sub/super-critical fluid chromatography (SFC). Racemic mixtures of 14 amino acids were used to evaluate the chiral recognition ability of 8 different polysaccharide-based chiral columns with coated or covalently immobilized chiral selectors. Among the studied columns, the one based on cellulose (3,5-dichlorophenylcarbamate) exhibited the most universal chiral recognition ability providing baseline resolution for the enantiomers of 10 studied amino acids. In addition, the effect of mobile phase composition (in particular, of the amount of additive used), as well as the potential contribution of evaporative light scattering detector (ELSD) to extra column band-broadening was evaluated.Show less >

Language :

Anglais

Audience :

Internationale

Popular science :

Non

Administrative institution(s) :

CHU Lille
Inserm
Université de Lille

Collections :

• Institut de Recherche Translationnelle sur l'Inflammation (INFINITE) - U1286

Submission date :

2019-10-22T08:16:33Z