Mucin CYS domain stiffens the mucus gel hindering bacteria and spermatozoa.

Demouveaux, Bastien; Gouyer, Valérie; Robbe-Masselot, Catherine; gottrand, frederic; Narita, Tetsuharu; Desseyn, Jean-Luc

Type de document :

Article dans une revue scientifique: Article original

DOI :

10.1038/s41598-019-53547-x

PMID :

31740753

URL permanente :

http://hdl.handle.net/20.500.12210/15786

Titre :

Mucin CYS domain stiffens the mucus gel hindering bacteria and spermatozoa.

Auteur(s) :

Demouveaux, Bastien [Auteur]

Lille Inflammation Research International Center - U 995 [LIRIC]
Gouyer, Valérie [Auteur]
Lille Inflammation Research International Center - U 995 [LIRIC]
Robbe-Masselot, Catherine [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
gottrand, frederic [Auteur]

Lille Inflammation Research International Center - U 995 [LIRIC]
Narita, Tetsuharu [Auteur]
Desseyn, Jean-Luc [Auteur]

Lille Inflammation Research International Center - U 995 [LIRIC]

Titre de la revue :

Scientific reports

Nom court de la revue :

Sci Rep

Numéro :

Pagination :

16993

Date de publication :

2019-11-18

ISSN :

2045-2322

Discipline(s) HAL :

Sciences du Vivant [q-bio]

Résumé en anglais : [en]

Mucus is the first biological barrier encountered by particles and pathogenic bacteria at the surface of secretory epithelia. The viscoelasticity of mucus is governed in part by low energy interactions that are difficult ...
Lire la suite >Mucus is the first biological barrier encountered by particles and pathogenic bacteria at the surface of secretory epithelia. The viscoelasticity of mucus is governed in part by low energy interactions that are difficult to assess. The CYS domain is a good candidate to support low energy interactions between GFMs and/or mucus constituents. Our aim was to stiffen the mucus from HT29-MTX cell cocultures and the colon of mice through the delivery of a recombinant protein made of hydrophobic CYS domains and found in multiple copies in polymeric mucins. The ability of the delivery of a poly-CYS molecule to stiffen mucus gels was assessed by probing cellular motility and particle diffusion. We demonstrated that poly-CYS enrichment decreases mucus permeability and hinders displacement of pathogenic flagellated bacteria and spermatozoa. Particle tracking microrheology showed a decrease of mucus diffusivity. The empirical obstruction scaling model evidenced a decrease of mesh size for mouse mucus enriched with poly-CYS molecules. Our data bring evidence that enrichment with a protein made of CYS domains stiffens the mucin network to provide a more impermeable and protective mucus barrier than mucus without such enrichment.Lire moins >

Langue :

Anglais

Comité de lecture :

Oui

Audience :

Non spécifiée

Établissement(s) :

Université de Lille
Inserm
CHU Lille
CNRS

Collections :

Équipe(s) de recherche :

Génétique des enveloppes bactériennes

Date de dépôt :

2019-11-25T15:57:25Z
2019-11-26T13:59:07Z
2021-01-06T15:18:17Z

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Ce document est diffusé sous licence Attribution 3.0 United States

Historique des modifications

Numéro de version	Lien	Date de modification
4	20.500.12210/15786*	2021-01-06T15:17:26Z
3	20.500.12210/15786.3	2019-11-26T13:53:53Z
1	20.500.12210/15786.1	2019-11-25T15:57:25Z

*Version sélectionnée

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