Diversity and trends in population structure ...
Document type :
Article dans une revue scientifique: Article original
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Title :
Diversity and trends in population structure of esbl-producing enterobacteriaceae in febrile urinary tract infections in children in france from 2014 to 2017
Author(s) :
Birgy, Andre [Auteur]
Madhi, Fouad [Auteur]
Jung, Camille [Auteur]
Levy, Corinne [Auteur]
Cointe, Aurelie [Auteur]
Bidet, Philippe [Auteur]
Hobson, Claire Amaris [Auteur]
Bechet, Stéphane [Auteur]
Sobral, Elsa [Auteur]
Vuthien, Hoang [Auteur]
Ferroni, Agnes [Auteur]
Aberrane, Said [Auteur]
Cuzon, Gaelle [Auteur]
Beraud, Laetitia [Auteur]
Gajdos, Vincent [Auteur]
Launay, Elise [Auteur]
Pinquier, Didier [Auteur]
Haas, Herve [Auteur]
Desmarest, Marie [Auteur]
Dommergues, Marie-Aliette [Auteur]
Cohen, Robert [Auteur]
Bonacorsi, Stéphane [Auteur]
Madhi, Fouad [Auteur]
Jung, Camille [Auteur]
Levy, Corinne [Auteur]
Cointe, Aurelie [Auteur]
Bidet, Philippe [Auteur]
Hobson, Claire Amaris [Auteur]
Bechet, Stéphane [Auteur]
Sobral, Elsa [Auteur]
Vuthien, Hoang [Auteur]
Ferroni, Agnes [Auteur]
Aberrane, Said [Auteur]
Cuzon, Gaelle [Auteur]
Beraud, Laetitia [Auteur]
Gajdos, Vincent [Auteur]
Launay, Elise [Auteur]
Pinquier, Didier [Auteur]
Haas, Herve [Auteur]
Desmarest, Marie [Auteur]
Dommergues, Marie-Aliette [Auteur]
Cohen, Robert [Auteur]
Bonacorsi, Stéphane [Auteur]
Journal title :
The Journal of antimicrobial chemotherapy
Abbreviated title :
J. Antimicrob. Chemother.
Publication date :
2019-10-16
ISSN :
1460-2091
HAL domain(s) :
Sciences du Vivant [q-bio]
English abstract : [en]
BACKGROUND: The population structure of extraintestinal pathogenic Escherichia coli evolves over time, notably due to the emergence of antibiotic-resistant clones such as ESBL-producing Enterobacteriaceae (ESBL-E).
OBJECTIVE: ...
Show more >BACKGROUND: The population structure of extraintestinal pathogenic Escherichia coli evolves over time, notably due to the emergence of antibiotic-resistant clones such as ESBL-producing Enterobacteriaceae (ESBL-E). OBJECTIVE: To analyse by WGS the genetic diversity of a large number of ESBL-E isolated from urinary tract infections in children from paediatric centres across France between 2014 and 2017 and collected by the National Observatory of febrile urinary tract infection (FUTI) caused by ESBL-E. METHODS: A total of 40 905 Enterobacteriaceae-positive urine cultures were identified. ESBL-E were found in 1983 samples (4.85%). WGS was performed on 251 ESBL-E causing FUTI. STs, core genome MLST (cgMLST), serotype, fimH allele, ESBL genes and presence of papGII key virulence factor were determined. RESULTS: E. coli and Klebsiella pneumoniae were found in 86.9% (218/251) and 11.2% (28/251) of cases, respectively. Several STs predominate among E. coli such as ST131, ST38, ST69, ST73, ST95, ST405, ST12 and ST1193, while no ST emerged in K. pneumoniae. E. coli ST131, ST38 and ST1193 increased during the study period, with a heterogeneity in papGII prevalence (64.5%, 35% and 20% respectively). Most isolates harboured the CTX-M type (97%) with a predominance of blaCTX-M-15. blaCTX-M-27, an emerging variant in E. coli, is found in various STs. cgMLST enabled discrimination of clusters within the main STs. CONCLUSIONS: The predominance of ST131, and the emergence of other STs such as ST38 and ST1193 combined with ESBL genes deserves close epidemiological surveillance considering their high threat in infectious disease. cgMLST could be a discriminant complementary tool for the analyses.Show less >
Show more >BACKGROUND: The population structure of extraintestinal pathogenic Escherichia coli evolves over time, notably due to the emergence of antibiotic-resistant clones such as ESBL-producing Enterobacteriaceae (ESBL-E). OBJECTIVE: To analyse by WGS the genetic diversity of a large number of ESBL-E isolated from urinary tract infections in children from paediatric centres across France between 2014 and 2017 and collected by the National Observatory of febrile urinary tract infection (FUTI) caused by ESBL-E. METHODS: A total of 40 905 Enterobacteriaceae-positive urine cultures were identified. ESBL-E were found in 1983 samples (4.85%). WGS was performed on 251 ESBL-E causing FUTI. STs, core genome MLST (cgMLST), serotype, fimH allele, ESBL genes and presence of papGII key virulence factor were determined. RESULTS: E. coli and Klebsiella pneumoniae were found in 86.9% (218/251) and 11.2% (28/251) of cases, respectively. Several STs predominate among E. coli such as ST131, ST38, ST69, ST73, ST95, ST405, ST12 and ST1193, while no ST emerged in K. pneumoniae. E. coli ST131, ST38 and ST1193 increased during the study period, with a heterogeneity in papGII prevalence (64.5%, 35% and 20% respectively). Most isolates harboured the CTX-M type (97%) with a predominance of blaCTX-M-15. blaCTX-M-27, an emerging variant in E. coli, is found in various STs. cgMLST enabled discrimination of clusters within the main STs. CONCLUSIONS: The predominance of ST131, and the emergence of other STs such as ST38 and ST1193 combined with ESBL genes deserves close epidemiological surveillance considering their high threat in infectious disease. cgMLST could be a discriminant complementary tool for the analyses.Show less >
Language :
Anglais
Audience :
Internationale
Popular science :
Non
Administrative institution(s) :
CHU Lille
Université de Lille
Université de Lille
Submission date :
2019-12-09T16:50:54Z