Titre :

Comparison of two commercial quantitative PCR assays and correlation with the first WHO International Standard for human CMV

Auteur(s) :

Engelmann, Ilka [Auteur]
Laboratoire de Virologie - ULR 3610 [Laboratoire de Virologie]
Alidjinou, Enagnon Kazali [Auteur]
Laboratoire de virologie - ULR 3610
Lazrek, Mouna [Auteur]
Laboratoire de Virologie - ULR 3610 [Laboratoire de Virologie]
Ogiez, Judith [Auteur]
Laboratoire de Virologie - ULR 3610 [Laboratoire de Virologie]
Pouillaude, Jean-Marie [Auteur]
Laboratoire de Virologie - ULR 3610 [Laboratoire de Virologie]
Chazard, Emmanuel [Auteur]
Evaluation des technologies de santé et des pratiques médicales - ULR 2694 [METRICS]
Dewilde, Anny [Auteur]
Laboratoire de Virologie - ULR 3610 [Laboratoire de Virologie]
Hober, Didier [Auteur]
215293|||Laboratoire de Virologie - ULR 3610 [Laboratoire de Virologie] (VALID)

Titre de la revue :

Diagnostic Microbiology and Infectious Disease

Nom court de la revue :

Diagn. Microbiol. Infect. Dis.

Numéro :

91

Pagination :

27-33

Éditeur :

Elsevier

Date de publication :

2018-01-07

ISSN :

1879-0070

Mot(s)-clé(s) en anglais :

Viral load
CMV
Molecular testing
WHO standard
PCR

Discipline(s) HAL :

Sciences du Vivant [q-bio]

Résumé en anglais : [en]

Comparability between CMV assays could be facilitated by the first WHO International Standard for human CMV (standard). Standard dilutions were submitted to nucleic acid extraction with Versant kPCR Molecular systems SP ...
Lire la suite >Comparability between CMV assays could be facilitated by the first WHO International Standard for human CMV (standard). Standard dilutions were submitted to nucleic acid extraction with Versant kPCR Molecular systems SP or MagNA Pure LC System followed by the kPCR PLX™ CMV DNA (kPCR) or the CMV R-gene™ assay (R-gene), respectively; 139 clinical specimens were tested. Both assays correlated well with the standard (R2 > 0.96) and a matrix effect was observed. Quantitative results correlated reasonably between both assays for whole blood (R2 = 0.79) and well for other specimen types (R2 = 0.93). Quantification differences were within one log10 of the averaged log10 results for 25/27 blood specimens and for 32/33 other specimens. Calibration to the standard did not increase this percentage. In conclusion, results of both assays showed reasonable correlation with each other and good correlation with the standard. Calibration to the standard did not improve comparability of quantitative results.Lire moins >

Langue :

Anglais

Audience :

Internationale

Vulgarisation :

Non

Établissement(s) :

CHU Lille
Université de Lille

Collections :

• Laboratoire de virologie - ULR 3610
• METRICS : Evaluation des technologies de santé et des pratiques médicales - ULR 2694

Date de dépôt :

2020-02-11T09:07:36Z
2024-02-23T10:09:52Z