Titre :

Distribution of the O-acetyl groups and β-galactofuranose units in galactoxylomannans of the opportunistic fungus Cryptococcus neoformans

Auteur(s) :

Previato, Jose O. [Auteur]
Instituto de Biofísica Carlos Chagas Filho [Rio de Janeiro] [IBCCF / UFRJ]
Vinogradov, Evgeny [Auteur]
National Research Council of Canada [NRC]
Maes, Emmanuel [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576
Fonseca, Leonardo M. [Auteur]
Instituto de Biofísica Carlos Chagas Filho [Rio de Janeiro] [IBCCF / UFRJ]
Guerardel, Yann [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576
Oliveira, Priscila A. V. [Auteur]
Instituto de Biofísica Carlos Chagas Filho [Rio de Janeiro] [IBCCF / UFRJ]
Mendonça-Previato, Lucia [Auteur]
Instituto de Biofísica Carlos Chagas Filho [Rio de Janeiro] [IBCCF / UFRJ]

Titre de la revue :

Glycobiology

Nom court de la revue :

Glycobiology

Numéro :

27

Pagination :

582-592

Date de publication :

2017-06-01

ISSN :

1460-2423

Mot(s)-clé(s) en anglais :

Cryptococcus
galactofuranose
O-acetyl
capsular polysaccharides
galactoxylomannans

Discipline(s) HAL :

Chimie/Chimie théorique et/ou physique

Résumé en anglais : [en]

Galactoxylomannans (GalXMs) are a mixture of neutral and acidic capsular polysaccharides produced by the opportunistic fungus Cryptococcus neoformans that exhibit potent suppressive effects on the host immune system. ...
Lire la suite >Galactoxylomannans (GalXMs) are a mixture of neutral and acidic capsular polysaccharides produced by the opportunistic fungus Cryptococcus neoformans that exhibit potent suppressive effects on the host immune system. Previous studies describing the chemical structure of C. neoformans GalXMs have reported species without O-acetyl substituents. Herein we describe that C. neoformans grown in capsule-inducing medium produces highly O-acetylated GalXMs. The location of the O-acetyl groups was determined by nuclear magnetic resonance (NMR) spectroscopy. In the neutral GalXM (NGalXM), 80% of 3-linked mannose (α-Manp) residues present in side chains are acetylated at the O-2 position. In the acidic GalXM also termed glucuronoxylomannogalactan (GXMGal), 85% of the 3-linked α-Manp residues are acetylated either in the O-2 (75%) or in the O-6 (25%) position, but O-acetyl groups are not present at both positions simultaneously. In addition, NMR spectroscopy and methylation analysis showed that β-galactofuranose (β-Galf) units are linked to O-2 and O-3 positions of nonbranched α-galactopyranose (α-Galp) units present in the GalXMs backbone chain. These findings highlight new structural features of C. neoformans GalXMs. Among these features, the high degree of O-acetylation is of particular interest, since O-acetyl group-containing polysaccharides are known to possess a range of immunobiological activities.Lire moins >

Langue :

Anglais

Audience :

Non spécifiée

Établissement(s) :

CNRS
Université de Lille

Collections :

• Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576

Équipe(s) de recherche :

Chemical Glycobiology

Date de dépôt :

2020-02-12T15:11:06Z
2021-05-20T10:08:15Z