Structural studies of the cell wall ...
Document type :
Article dans une revue scientifique
PMID :
Permalink :
Title :
Structural studies of the cell wall polysaccharides from three strains of Lactobacillus helveticus with different autolytic properties: DPC4571, BROI, and LH1
Author(s) :
Vinogradov, Evgeny [Auteur]
National Research Council of Canada [NRC]
Valence, Florence [Auteur]
Science et Technologie du Lait et de l'Oeuf [STLO]
Maes, Emmanuel [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Jebava, Iva [Auteur]
Institute of Chemical Technology [Prague] [ICT]
Science et Technologie du Lait et de l'Oeuf [STLO]
Chuat, Victoria [Auteur]
Science et Technologie du Lait et de l'Oeuf [STLO]
Lortal, Sylvie [Auteur]
Science et Technologie du Lait et de l'Oeuf [STLO]
Grard, Thierry [Auteur]
Université du Littoral Côte d'Opale [ULCO]
Guerardel, Yann [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Sadovskaya, Irina [Auteur]
Université du Littoral Côte d'Opale [ULCO]
National Research Council of Canada [NRC]
Valence, Florence [Auteur]
Science et Technologie du Lait et de l'Oeuf [STLO]
Maes, Emmanuel [Auteur]

Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Jebava, Iva [Auteur]
Institute of Chemical Technology [Prague] [ICT]
Science et Technologie du Lait et de l'Oeuf [STLO]
Chuat, Victoria [Auteur]
Science et Technologie du Lait et de l'Oeuf [STLO]
Lortal, Sylvie [Auteur]
Science et Technologie du Lait et de l'Oeuf [STLO]
Grard, Thierry [Auteur]
Université du Littoral Côte d'Opale [ULCO]
Guerardel, Yann [Auteur]

Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Sadovskaya, Irina [Auteur]

Université du Littoral Côte d'Opale [ULCO]
Journal title :
Carbohydrate research
Abbreviated title :
Carbohydr. Res.
Volume number :
379
Pages :
7-12
Publication date :
2013-09-20
ISSN :
1873-426X
English keyword(s) :
Lactobacillus helveticus
Cell-wall polysaccharide
Structure
Autolytic properties
Cell-wall polysaccharide
Structure
Autolytic properties
HAL domain(s) :
Chimie/Chimie théorique et/ou physique
Sciences du Vivant [q-bio]
Sciences du Vivant [q-bio]/Microbiologie et Parasitologie
Sciences du Vivant [q-bio]
Sciences du Vivant [q-bio]/Microbiologie et Parasitologie
English abstract : [en]
Lactobacillus helveticus is traditionally used in dairy industry as a starter or an adjunct culture for manufacture of cheese and some types of fermented milk. Its autolysis releases intracellular enzymes which is a ...
Show more >Lactobacillus helveticus is traditionally used in dairy industry as a starter or an adjunct culture for manufacture of cheese and some types of fermented milk. Its autolysis releases intracellular enzymes which is a prerequisite for optimum cheese maturation, and is known to be strain dependent. Autolysis is caused by an enzymatic hydrolysis of the cell wall peptidoglycan (PG) by endogenous peptidoglycan hydrolases (PGHs) or autolysins. Origins of differences in autolytic properties of different strains are not fully elucidated. Regulation of autolysis possibly depends on the structure of the cell wall components other than PG, particularly polysaccharides. In the present work, we screened six L. helveticus strains with different autolytic properties: DPC4571, BROI and LH1. We established, for the first time, that cell walls (CWs) of these strains contained polysaccharides, different from their CW teichoic acids. Cell wall polysaccharides of three strains were purified, and their chemical structures were established by 2D NMR spectroscopy and methylation analysis. The structures of their repeating units are presented.Show less >
Show more >Lactobacillus helveticus is traditionally used in dairy industry as a starter or an adjunct culture for manufacture of cheese and some types of fermented milk. Its autolysis releases intracellular enzymes which is a prerequisite for optimum cheese maturation, and is known to be strain dependent. Autolysis is caused by an enzymatic hydrolysis of the cell wall peptidoglycan (PG) by endogenous peptidoglycan hydrolases (PGHs) or autolysins. Origins of differences in autolytic properties of different strains are not fully elucidated. Regulation of autolysis possibly depends on the structure of the cell wall components other than PG, particularly polysaccharides. In the present work, we screened six L. helveticus strains with different autolytic properties: DPC4571, BROI and LH1. We established, for the first time, that cell walls (CWs) of these strains contained polysaccharides, different from their CW teichoic acids. Cell wall polysaccharides of three strains were purified, and their chemical structures were established by 2D NMR spectroscopy and methylation analysis. The structures of their repeating units are presented.Show less >
Language :
Anglais
Audience :
Non spécifiée
Administrative institution(s) :
CNRS
INRA
ISA
Univ. Artois
Univ. Littoral Côte d’Opale
Université de Lille
INRA
ISA
Univ. Artois
Univ. Littoral Côte d’Opale
Université de Lille
Research team(s) :
Chemical Glycobiology
Submission date :
2020-02-12T15:11:08Z
2021-03-03T07:31:11Z
2021-03-03T07:47:13Z
2021-03-03T07:31:11Z
2021-03-03T07:47:13Z