Analysis of the σE regulon in Crohn's ...
Type de document :
Article dans une revue scientifique
DOI :
PMID :
URL permanente :
Titre :
Analysis of the σE regulon in Crohn's disease-associated Escherichia coli revealed involvement of the waaWVL operon in biofilm formation
Auteur(s) :
Chassaing, Benoit [Auteur]
Institut National de la Recherche Agronomique [INRA]
Institut National de la Santé et de la Recherche Médicale [INSERM]
Garénaux, Estelle [Auteur]
Institut National de la Recherche Agronomique [INRA]
Institut National de la Santé et de la Recherche Médicale [INSERM]
Carriere, Jessica [Auteur]
Institut National de la Recherche Agronomique [INRA]
Institut National de la Santé et de la Recherche Médicale [INSERM]
Rolhion, Nathalie [Auteur]
Institut National de la Recherche Agronomique [INRA]
Institut National de la Santé et de la Recherche Médicale [INSERM]
Guerardel, Yann [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 [UGSF]
Barnich, Nicolas [Auteur]
Institut National de la Santé et de la Recherche Médicale [INSERM]
Institut National de la Recherche Agronomique [INRA]
Bonnet, Richard [Auteur]
Institut National de la Recherche Agronomique [INRA]
Institut National de la Santé et de la Recherche Médicale [INSERM]
Darfeuille-Michaud, Arlette [Auteur]
Institut National de la Recherche Agronomique [INRA]
Institut National de la Santé et de la Recherche Médicale [INSERM]
Institut National de la Recherche Agronomique [INRA]
Institut National de la Santé et de la Recherche Médicale [INSERM]
Garénaux, Estelle [Auteur]
Institut National de la Recherche Agronomique [INRA]
Institut National de la Santé et de la Recherche Médicale [INSERM]
Carriere, Jessica [Auteur]
Institut National de la Recherche Agronomique [INRA]
Institut National de la Santé et de la Recherche Médicale [INSERM]
Rolhion, Nathalie [Auteur]
Institut National de la Recherche Agronomique [INRA]
Institut National de la Santé et de la Recherche Médicale [INSERM]
Guerardel, Yann [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 [UGSF]
Barnich, Nicolas [Auteur]
Institut National de la Santé et de la Recherche Médicale [INSERM]
Institut National de la Recherche Agronomique [INRA]
Bonnet, Richard [Auteur]
Institut National de la Recherche Agronomique [INRA]
Institut National de la Santé et de la Recherche Médicale [INSERM]
Darfeuille-Michaud, Arlette [Auteur]
Institut National de la Recherche Agronomique [INRA]
Institut National de la Santé et de la Recherche Médicale [INSERM]
Titre de la revue :
Journal of Bacteriology
Nom court de la revue :
J. Bacteriol.
Numéro :
197
Pagination :
1451-1465
Date de publication :
2015-04
ISSN :
1098-5530
Discipline(s) HAL :
Chimie/Chimie théorique et/ou physique
Sciences du Vivant [q-bio]
Sciences du Vivant [q-bio]
Résumé en anglais : [en]
Ileal lesions of patients with Crohn's disease are colonized by adherent-invasive Escherichia coli (AIEC), which is able to adhere to and to invade intestinal epithelial cells (IEC), to replicate within macrophages, and ...
Lire la suite >Ileal lesions of patients with Crohn's disease are colonized by adherent-invasive Escherichia coli (AIEC), which is able to adhere to and to invade intestinal epithelial cells (IEC), to replicate within macrophages, and to form biofilms on the surface of the intestinal mucosa. Previous analyses indicated the involvement of the σ(E) pathway in AIEC-IEC interaction, as well as in biofilm formation, with σ(E) pathway inhibition leading to an impaired ability of AIEC to colonize the intestinal mucosa and to form biofilms. The aim of this study was to characterize the σ(E) regulon of AIEC strain LF82 in order to identify members involved in AIEC phenotypes. Using comparative in silico analysis of the σ(E) regulon, we identified the waaWVL operon as a new member of the σ(E) regulon in reference AIEC strain LF82. We determined that the waaWVL operon is involved in AIEC lipopolysaccharide structure and composition, and the waaWVL operon was found to be essential for AIEC strains to produce biofilm and to colonize the intestinal mucosa. IMPORTANCE: An increased prevalence of adherent-invasive Escherichia coli (AIEC) bacteria was previously observed in the intestinal mucosa of Crohn's disease (CD) patients, and clinical observations revealed bacterial biofilms associated with the mucosa of CD patients. Here, analysis of the σ(E) regulon in AIEC and commensal E. coli identified 12 genes controlled by σ(E) only in AIEC. Among them, WaaWVL factors were found to play an essential role in biofilm formation and mucosal colonization by AIEC. In addition to identifying molecular tools that revealed a pathogenic population of E. coli colonizing the mucosa of CD patients, these results indicate that targeting the waaWVL operon could be a potent therapeutic strategy to interfere with the ability of AIEC to form biofilms and to colonize the gut mucosa.Lire moins >
Lire la suite >Ileal lesions of patients with Crohn's disease are colonized by adherent-invasive Escherichia coli (AIEC), which is able to adhere to and to invade intestinal epithelial cells (IEC), to replicate within macrophages, and to form biofilms on the surface of the intestinal mucosa. Previous analyses indicated the involvement of the σ(E) pathway in AIEC-IEC interaction, as well as in biofilm formation, with σ(E) pathway inhibition leading to an impaired ability of AIEC to colonize the intestinal mucosa and to form biofilms. The aim of this study was to characterize the σ(E) regulon of AIEC strain LF82 in order to identify members involved in AIEC phenotypes. Using comparative in silico analysis of the σ(E) regulon, we identified the waaWVL operon as a new member of the σ(E) regulon in reference AIEC strain LF82. We determined that the waaWVL operon is involved in AIEC lipopolysaccharide structure and composition, and the waaWVL operon was found to be essential for AIEC strains to produce biofilm and to colonize the intestinal mucosa. IMPORTANCE: An increased prevalence of adherent-invasive Escherichia coli (AIEC) bacteria was previously observed in the intestinal mucosa of Crohn's disease (CD) patients, and clinical observations revealed bacterial biofilms associated with the mucosa of CD patients. Here, analysis of the σ(E) regulon in AIEC and commensal E. coli identified 12 genes controlled by σ(E) only in AIEC. Among them, WaaWVL factors were found to play an essential role in biofilm formation and mucosal colonization by AIEC. In addition to identifying molecular tools that revealed a pathogenic population of E. coli colonizing the mucosa of CD patients, these results indicate that targeting the waaWVL operon could be a potent therapeutic strategy to interfere with the ability of AIEC to form biofilms and to colonize the gut mucosa.Lire moins >
Langue :
Anglais
Audience :
Non spécifiée
Établissement(s) :
CNRS
Université de Lille
Université de Lille
Équipe(s) de recherche :
Chemical Glycobiology
Date de dépôt :
2020-02-12T15:11:09Z
2021-03-03T07:18:27Z
2021-03-03T07:22:04Z
2021-03-03T07:18:27Z
2021-03-03T07:22:04Z