Title :

BLISS: A Bioorthogonal Dual-Labeling Strategy to Unravel Lignification Dynamics in Plants

Author(s) :

Lion, Cédric [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576
Simon, Clémence [Auteur]
Huss, Brigitte [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576
Blervacq, Anne-Sophie [Auteur]
Tirot, Louis [Auteur]
Toybou, Djadidi [Auteur]
Spriet, Corentin [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576
Slomianny, Christian [Auteur]
Guerardel, Yann [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576
Hawkins, Simon [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576
Biot, Christophe [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576

Journal title :

Cell Chemical Biology

Abbreviated title :

Cell Chem Biol

Volume number :

24

Pages :

326-338

Publication date :

2017-03-16

ISSN :

2451-9456

English keyword(s) :

lignification
SPAAC
Click Chemistry
CuAAC
bioorthogonal chemistry
dual labeling
lignin
monolignol
Metabolic labeling
plant cell wall

HAL domain(s) :

Chimie/Chimie théorique et/ou physique

English abstract : [en]

A better in vivo understanding of lignin formation within plant cell walls will contribute to improving the valorization of plant-derived biomass. Although bioorthogonal chemistry provides a promising platform to study the ...
Show more >A better in vivo understanding of lignin formation within plant cell walls will contribute to improving the valorization of plant-derived biomass. Although bioorthogonal chemistry provides a promising platform to study the lignification process, methodologies that simultaneously detect multiple chemical reporters in living organisms are still scarce. Here, we have developed an original bioorthogonal labeling imaging sequential strategy (BLISS) to visualize and analyze the incorporation of both p-hydroxyphenyl (H) and guaiacyl (G) units into lignin in vivo with a combination of strain-promoted and copper-catalyzed azide-alkyne cycloadditions. On our path to BLISS, we designed a new azide-tagged monolignol reporter for H units in metabolic lignin engineering and used it in conjunction with an alkyne-tagged G unit surrogate to study lignification dynamics in flax. Here, we show that BLISS provides precise spatial information on the zones of active lignification and reveals polarization in single-cell lignification dynamics.Show less >

Language :

Anglais

Administrative institution(s) :

CNRS
Inserm
Université de Lille

Collections :

• Physiologie Cellulaire (PHYCEL) - U1003
• Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576

Research team(s) :

Fibres végétales
Glycobiologie structurale des interactions hôtes-pathogènes

Submission date :

2020-02-12T15:11:12Z