Increased phagocytosis of Mycobacterium ...
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Article dans une revue scientifique
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Title :
Increased phagocytosis of Mycobacterium marinum mutants defective in lipooligosaccharide production: a structure-activity relationship study
Author(s) :
Alibaud, Laeticia [Auteur]
Dynamique des interactions membranaires normales et pathologiques [DIMNP]
Pawelczyk, Jakub [Auteur]
Polska Akademia Nauk = Polish Academy of Sciences = Académie polonaise des sciences [PAN]
Gannoun-Zaki, Laila [Auteur]
Dynamique des interactions membranaires normales et pathologiques [DIMNP]
Singh, Vipul K. [Auteur]
Dynamique des interactions membranaires normales et pathologiques [DIMNP]
Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes [URMITE]
Rombouts, Yoann [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Drancourt, Michel [Auteur]
Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes [URMITE]
Dziadek, Jaroslaw [Auteur]
Polska Akademia Nauk = Polish Academy of Sciences = Académie polonaise des sciences [PAN]
Guerardel, Yann [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Kremer, Laurent [Auteur]
Dynamique des interactions membranaires normales et pathologiques [DIMNP]
Dynamique des interactions membranaires normales et pathologiques [DIMNP]
Pawelczyk, Jakub [Auteur]
Polska Akademia Nauk = Polish Academy of Sciences = Académie polonaise des sciences [PAN]
Gannoun-Zaki, Laila [Auteur]
Dynamique des interactions membranaires normales et pathologiques [DIMNP]
Singh, Vipul K. [Auteur]
Dynamique des interactions membranaires normales et pathologiques [DIMNP]
Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes [URMITE]
Rombouts, Yoann [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Drancourt, Michel [Auteur]
Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes [URMITE]
Dziadek, Jaroslaw [Auteur]
Polska Akademia Nauk = Polish Academy of Sciences = Académie polonaise des sciences [PAN]
Guerardel, Yann [Auteur]

Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Kremer, Laurent [Auteur]
Dynamique des interactions membranaires normales et pathologiques [DIMNP]
Journal title :
The Journal of biological chemistry
Abbreviated title :
J. Biol. Chem.
Volume number :
289
Pages :
215-228
Publication date :
2014-01-03
ISSN :
1083-351X
English keyword(s) :
Cell Line
Genes, Bacterial
Mycobacterium
Mycobacterium marinum
Humans
Mass Spectrometry (MS)
Lipooligosaccharide
Macrophages
Carbohydrate Biosynthesis
Mycobacterium Infections, Nontuberculous
Lipopolysaccharides
Glycolipid Structure
Acanthamoeba
Mutation
Thin Layer Chromatography
Phagocytosis
Genes, Bacterial
Mycobacterium
Mycobacterium marinum
Humans
Mass Spectrometry (MS)
Lipooligosaccharide
Macrophages
Carbohydrate Biosynthesis
Mycobacterium Infections, Nontuberculous
Lipopolysaccharides
Glycolipid Structure
Acanthamoeba
Mutation
Thin Layer Chromatography
Phagocytosis
HAL domain(s) :
Chimie/Chimie théorique et/ou physique
English abstract : [en]
Mycobacterium marinum is a waterborne pathogen responsible for tuberculosis-like infections in ectotherms and is an occasional opportunistic human pathogen. In the environment, M. marinum also interacts with amoebae, which ...
Show more >Mycobacterium marinum is a waterborne pathogen responsible for tuberculosis-like infections in ectotherms and is an occasional opportunistic human pathogen. In the environment, M. marinum also interacts with amoebae, which may serve as a natural reservoir for this microorganism. However, the description of mycobacterial determinants in the early interaction with macrophages or amoebae remains elusive. Lipooligosaccharides (LOSs) are cell surface-exposed glycolipids capable of modulating the host immune system, suggesting that they may be involved in the early interactions of M. marinum with macrophages. Herein, we addressed whether LOS composition affects the uptake of M. marinum by professional phagocytes. Mutants with various truncated LOS variants were generated, leading to the identification of several previously uncharacterized biosynthetic genes (wbbL2, MMAR_2321, and MMAR_2331). Biochemical and structural approaches allowed resolving the structures of LOS precursors accumulating in this set of mutants. These strains with structurally defined LOS profiles were then used to infect both macrophages and Acanthamoebae. An inverse correlation between LOS completeness and uptake of mycobacteria by phagocytes was found, allowing the proposal of three mutant classes: class I (papA4), devoid of LOS and highly efficiently phagocytosed; class II, accumulating only early LOS intermediates (wbbL2 and MMAR_2331) and efficiently phagocytosed but less than class I mutants; class III, lacking LOS-IV (losA, MMAR_2319, and MMAR_2321) and phagocytosed similarly to the control strain. These results indicate that phagocytosis is conditioned by the LOS pattern and that the LOS pathway used by M. marinum in macrophages is conserved during infection of amoebae.Show less >
Show more >Mycobacterium marinum is a waterborne pathogen responsible for tuberculosis-like infections in ectotherms and is an occasional opportunistic human pathogen. In the environment, M. marinum also interacts with amoebae, which may serve as a natural reservoir for this microorganism. However, the description of mycobacterial determinants in the early interaction with macrophages or amoebae remains elusive. Lipooligosaccharides (LOSs) are cell surface-exposed glycolipids capable of modulating the host immune system, suggesting that they may be involved in the early interactions of M. marinum with macrophages. Herein, we addressed whether LOS composition affects the uptake of M. marinum by professional phagocytes. Mutants with various truncated LOS variants were generated, leading to the identification of several previously uncharacterized biosynthetic genes (wbbL2, MMAR_2321, and MMAR_2331). Biochemical and structural approaches allowed resolving the structures of LOS precursors accumulating in this set of mutants. These strains with structurally defined LOS profiles were then used to infect both macrophages and Acanthamoebae. An inverse correlation between LOS completeness and uptake of mycobacteria by phagocytes was found, allowing the proposal of three mutant classes: class I (papA4), devoid of LOS and highly efficiently phagocytosed; class II, accumulating only early LOS intermediates (wbbL2 and MMAR_2331) and efficiently phagocytosed but less than class I mutants; class III, lacking LOS-IV (losA, MMAR_2319, and MMAR_2321) and phagocytosed similarly to the control strain. These results indicate that phagocytosis is conditioned by the LOS pattern and that the LOS pathway used by M. marinum in macrophages is conserved during infection of amoebae.Show less >
Language :
Anglais
Audience :
Non spécifiée
Administrative institution(s) :
CNRS
Université de Lille
Université de Lille
Research team(s) :
Chemical Glycobiology
Submission date :
2020-02-12T15:11:12Z
2021-03-12T12:19:58Z
2021-03-12T12:19:58Z
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