Surface Plasmon Resonance (SPR) for the ...
Document type :
Article dans une revue scientifique
DOI :
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Title :
Surface Plasmon Resonance (SPR) for the Evaluation of Shear-Force-Dependent Bacterial Adhesion
Author(s) :
Zagorodko, Oleksandr [Auteur]
Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 [IEMN]
Bouckaert, Julie [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 [UGSF]
Dumych, Tetiana [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Bilyy, Rostyslav [Auteur]
National Academy of Sciences of Ukraine [NASU]
Larroulet, Iban [Auteur]
Yanguas Serrano, Aritz [Auteur]
Alvarez Dorta, Dimitri [Auteur]
Chimie Et Interdisciplinarité : Synthèse, Analyse, Modélisation [CEISAM]
Gouin, Sébastien G. [Auteur]
Chimie Et Interdisciplinarité : Synthèse, Analyse, Modélisation [CEISAM]
Dima, Stefan-Ovidiu [Auteur]
The National Institute for Research & Development in Chemistry and Petrochemistry [ICECHIM]
Oancea, Florin [Auteur]
The National Institute for Research & Development in Chemistry and Petrochemistry [ICECHIM]
Boukherroub, Rabah [Auteur]
Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 [IEMN]
Szunerits, Sabine [Auteur]
Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 [IEMN]
Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 [IEMN]
Bouckaert, Julie [Auteur]

Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 [UGSF]
Dumych, Tetiana [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Bilyy, Rostyslav [Auteur]
National Academy of Sciences of Ukraine [NASU]
Larroulet, Iban [Auteur]
Yanguas Serrano, Aritz [Auteur]
Alvarez Dorta, Dimitri [Auteur]
Chimie Et Interdisciplinarité : Synthèse, Analyse, Modélisation [CEISAM]
Gouin, Sébastien G. [Auteur]
Chimie Et Interdisciplinarité : Synthèse, Analyse, Modélisation [CEISAM]
Dima, Stefan-Ovidiu [Auteur]
The National Institute for Research & Development in Chemistry and Petrochemistry [ICECHIM]
Oancea, Florin [Auteur]
The National Institute for Research & Development in Chemistry and Petrochemistry [ICECHIM]
Boukherroub, Rabah [Auteur]

Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 [IEMN]
Szunerits, Sabine [Auteur]

Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 [IEMN]
Journal title :
Biosensors
Abbreviated title :
Biosensors (Basel)
Volume number :
5
Pages :
276-287
Publication date :
2015-05-26
ISSN :
2079-6374
English keyword(s) :
Bacterial Adhesion
carbohydrates
shear force enhancement
Surface Plasmon Resonance
Gold
Biomechanical Phenomena
Escherichia coli
Escherichia coli (E. coli)
Biosensing Techniques
Mannose
surface plasmon resonance (SPR)
flow rate
carbohydrates
shear force enhancement
Surface Plasmon Resonance
Gold
Biomechanical Phenomena
Escherichia coli
Escherichia coli (E. coli)
Biosensing Techniques
Mannose
surface plasmon resonance (SPR)
flow rate
HAL domain(s) :
Chimie/Chimie théorique et/ou physique
English abstract : [en]
The colonization of Escherichia coli (E. coli) to host cell surfaces is known to be a glycan-specific process that can be modulated by shear stress. In this work we investigate whether flow rate changes in microchannels ...
Show more >The colonization of Escherichia coli (E. coli) to host cell surfaces is known to be a glycan-specific process that can be modulated by shear stress. In this work we investigate whether flow rate changes in microchannels integrated on surface plasmon resonance (SPR) surfaces would allow for investigating such processes in an easy and high-throughput manner. We demonstrate that adhesion of uropathogenic E. coli UTI89 on heptyl α-d-mannopyranoside-modified gold SPR substrates is minimal under almost static conditions (flow rates of 10 µL·min⁻¹), and reaches a maximum at flow rates of 30 µL·min⁻¹ (≈30 mPa). This concept is applicable to the investigation of any ligand-pathogen interactions, offering a robust, easy, and fast method for screening adhesion characteristics of pathogens to ligand-modified interfaces.Show less >
Show more >The colonization of Escherichia coli (E. coli) to host cell surfaces is known to be a glycan-specific process that can be modulated by shear stress. In this work we investigate whether flow rate changes in microchannels integrated on surface plasmon resonance (SPR) surfaces would allow for investigating such processes in an easy and high-throughput manner. We demonstrate that adhesion of uropathogenic E. coli UTI89 on heptyl α-d-mannopyranoside-modified gold SPR substrates is minimal under almost static conditions (flow rates of 10 µL·min⁻¹), and reaches a maximum at flow rates of 30 µL·min⁻¹ (≈30 mPa). This concept is applicable to the investigation of any ligand-pathogen interactions, offering a robust, easy, and fast method for screening adhesion characteristics of pathogens to ligand-modified interfaces.Show less >
Language :
Anglais
Audience :
Non spécifiée
Administrative institution(s) :
ISEN
Univ. Valenciennes
CNRS
Institut Catholique Lille
Centrale Lille
Université de Lille
Univ. Valenciennes
CNRS
Institut Catholique Lille
Centrale Lille
Université de Lille
Collections :
Research team(s) :
Computational Molecular Systems Biology
Submission date :
2020-02-12T15:11:18Z
2021-03-18T09:01:07Z
2021-03-18T09:01:07Z