The level of Ets-1 protein is regulated ...
Document type :
Article dans une revue scientifique
PMID :
Permalink :
Title :
The level of Ets-1 protein is regulated by poly(ADP-ribose) polymerase-1 (PARP-1) in cancer cells to prevent DNA damage
Author(s) :
Legrand, Arnaud J. [Auteur]
Institut de Recherche Interdisciplinaire [Villeneuve d'Ascq] [IRI]
Choul-Li, Souhaila [Auteur]
Institut Pasteur de Lille
Spriet, Corentin [Auteur]
Institut de Recherche Interdisciplinaire [Villeneuve d'Ascq] [IRI]
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Idziorek, Thierry [Auteur]
Vicogne, Dorothee [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Institut de Recherche Interdisciplinaire [Villeneuve d'Ascq] [IRI]
Drobecq, Herve [Auteur]
Institut Pasteur de Lille
Dantzer, Françoise [Auteur]
Biotechnologie et signalisation cellulaire [BSC]
Villeret, Vincent [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Institut de Recherche Interdisciplinaire [Villeneuve d'Ascq] [IRI]
Aumercier, Marc [Auteur]
Institut de Recherche Interdisciplinaire [Villeneuve d'Ascq] [IRI]
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Institut de Recherche Interdisciplinaire [Villeneuve d'Ascq] [IRI]
Choul-Li, Souhaila [Auteur]
Institut Pasteur de Lille
Spriet, Corentin [Auteur]
Institut de Recherche Interdisciplinaire [Villeneuve d'Ascq] [IRI]
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Idziorek, Thierry [Auteur]
Vicogne, Dorothee [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Institut de Recherche Interdisciplinaire [Villeneuve d'Ascq] [IRI]
Drobecq, Herve [Auteur]
Institut Pasteur de Lille
Dantzer, Françoise [Auteur]
Biotechnologie et signalisation cellulaire [BSC]
Villeret, Vincent [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Institut de Recherche Interdisciplinaire [Villeneuve d'Ascq] [IRI]
Aumercier, Marc [Auteur]
Institut de Recherche Interdisciplinaire [Villeneuve d'Ascq] [IRI]
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Journal title :
PLoS One
Abbreviated title :
PLoS ONE
Volume number :
8
Pages :
e55883
Publication date :
2013-02
ISSN :
1932-6203
English keyword(s) :
Immunoprecipitation
Poly(ADP-ribose) Polymerases
Humans
Cells, Cultured
Breast Neoplasms
Poly Adenosine Diphosphate Ribose
Poly(ADP-ribose) Polymerase Inhibitors
Blotting, Western
Mice, Knockout
Animals
Flow Cytometry
Fluorescent Antibody Technique
Female
Transcription, Genetic
Mice
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Proto-Oncogene Protein c-ets-1
DNA Damage
RNA, Messenger
Cell Nucleus
Poly(ADP-ribose) Polymerases
Humans
Cells, Cultured
Breast Neoplasms
Poly Adenosine Diphosphate Ribose
Poly(ADP-ribose) Polymerase Inhibitors
Blotting, Western
Mice, Knockout
Animals
Flow Cytometry
Fluorescent Antibody Technique
Female
Transcription, Genetic
Mice
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Proto-Oncogene Protein c-ets-1
DNA Damage
RNA, Messenger
Cell Nucleus
HAL domain(s) :
Chimie/Chimie théorique et/ou physique
English abstract : [en]
Ets-1 is a transcription factor that regulates many genes involved in cancer progression and in tumour invasion. It is a poor prognostic marker for breast, lung, colorectal and ovary carcinomas. Here, we identified ...
Show more >Ets-1 is a transcription factor that regulates many genes involved in cancer progression and in tumour invasion. It is a poor prognostic marker for breast, lung, colorectal and ovary carcinomas. Here, we identified poly(ADP-ribose) polymerase-1 (PARP-1) as a novel interaction partner of Ets-1. We show that Ets-1 activates, by direct interaction, the catalytic activity of PARP-1 and is then poly(ADP-ribosyl)ated in a DNA-independent manner. The catalytic inhibition of PARP-1 enhanced Ets-1 transcriptional activity and caused its massive accumulation in cell nuclei. Ets-1 expression was correlated with an increase in DNA damage when PARP-1 was inhibited, leading to cancer cell death. Moreover, PARP-1 inhibitors caused only Ets-1-expressing cells to accumulate DNA damage. These results provide new insight into Ets-1 regulation in cancer cells and its link with DNA repair proteins. Furthermore, our findings suggest that PARP-1 inhibitors would be useful in a new therapeutic strategy that specifically targets Ets-1-expressing tumours.Show less >
Show more >Ets-1 is a transcription factor that regulates many genes involved in cancer progression and in tumour invasion. It is a poor prognostic marker for breast, lung, colorectal and ovary carcinomas. Here, we identified poly(ADP-ribose) polymerase-1 (PARP-1) as a novel interaction partner of Ets-1. We show that Ets-1 activates, by direct interaction, the catalytic activity of PARP-1 and is then poly(ADP-ribosyl)ated in a DNA-independent manner. The catalytic inhibition of PARP-1 enhanced Ets-1 transcriptional activity and caused its massive accumulation in cell nuclei. Ets-1 expression was correlated with an increase in DNA damage when PARP-1 was inhibited, leading to cancer cell death. Moreover, PARP-1 inhibitors caused only Ets-1-expressing cells to accumulate DNA damage. These results provide new insight into Ets-1 regulation in cancer cells and its link with DNA repair proteins. Furthermore, our findings suggest that PARP-1 inhibitors would be useful in a new therapeutic strategy that specifically targets Ets-1-expressing tumours.Show less >
Language :
Anglais
Audience :
Non spécifiée
Administrative institution(s) :
CHU Lille
CNRS
Inserm
Université de Lille
CNRS
Inserm
Université de Lille
Collections :
Research team(s) :
Biologie structurale et intégrative
Submission date :
2020-02-12T15:11:32Z
2021-03-26T07:06:02Z
2021-03-26T07:06:02Z
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