Title :

The level of Ets-1 protein is regulated by poly(ADP-ribose) polymerase-1 (PARP-1) in cancer cells to prevent DNA damage

Author(s) :

Legrand, Arnaud J. [Auteur]
Institut de Recherche Interdisciplinaire [Villeneuve d'Ascq] [IRI]
Choul-Li, Souhaila [Auteur]
Institut Pasteur de Lille
Spriet, Corentin [Auteur]
Institut de Recherche Interdisciplinaire [Villeneuve d'Ascq] [IRI]
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Idziorek, Thierry [Auteur]

Vicogne, Dorothee [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Institut de Recherche Interdisciplinaire [Villeneuve d'Ascq] [IRI]
Drobecq, Herve [Auteur]
Institut Pasteur de Lille
Dantzer, Françoise [Auteur]
Biotechnologie et signalisation cellulaire [BSC]
Villeret, Vincent [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Institut de Recherche Interdisciplinaire [Villeneuve d'Ascq] [IRI]
Aumercier, Marc [Auteur]
Institut de Recherche Interdisciplinaire [Villeneuve d'Ascq] [IRI]
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]

Journal title :

PLoS One

Abbreviated title :

PLoS ONE

Volume number :

8

Pages :

e55883

Publication date :

2013-02

ISSN :

1932-6203

English keyword(s) :

Immunoprecipitation
Poly(ADP-ribose) Polymerases
Humans
Cells, Cultured
Breast Neoplasms
Poly Adenosine Diphosphate Ribose
Poly(ADP-ribose) Polymerase Inhibitors
Blotting, Western
Mice, Knockout
Animals
Flow Cytometry
Fluorescent Antibody Technique
Female
Transcription, Genetic
Mice
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Proto-Oncogene Protein c-ets-1
DNA Damage
RNA, Messenger
Cell Nucleus

HAL domain(s) :

Chimie/Chimie théorique et/ou physique

English abstract : [en]

Ets-1 is a transcription factor that regulates many genes involved in cancer progression and in tumour invasion. It is a poor prognostic marker for breast, lung, colorectal and ovary carcinomas. Here, we identified ...
Show more >Ets-1 is a transcription factor that regulates many genes involved in cancer progression and in tumour invasion. It is a poor prognostic marker for breast, lung, colorectal and ovary carcinomas. Here, we identified poly(ADP-ribose) polymerase-1 (PARP-1) as a novel interaction partner of Ets-1. We show that Ets-1 activates, by direct interaction, the catalytic activity of PARP-1 and is then poly(ADP-ribosyl)ated in a DNA-independent manner. The catalytic inhibition of PARP-1 enhanced Ets-1 transcriptional activity and caused its massive accumulation in cell nuclei. Ets-1 expression was correlated with an increase in DNA damage when PARP-1 was inhibited, leading to cancer cell death. Moreover, PARP-1 inhibitors caused only Ets-1-expressing cells to accumulate DNA damage. These results provide new insight into Ets-1 regulation in cancer cells and its link with DNA repair proteins. Furthermore, our findings suggest that PARP-1 inhibitors would be useful in a new therapeutic strategy that specifically targets Ets-1-expressing tumours.Show less >

Language :

Anglais

Audience :

Non spécifiée

Administrative institution(s) :

CHU Lille
CNRS
Inserm
Université de Lille

Collections :

• Lille Neurosciences & Cognition (LilNCog) - U 1172
• Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576

Research team(s) :

Biologie structurale et intégrative

Submission date :

2020-02-12T15:11:32Z
2021-03-26T07:06:02Z