Apical membrane expression of distinct ...
Type de document :
Article dans une revue scientifique
PMID :
URL permanente :
Titre :
Apical membrane expression of distinct sulfated glycans represents a novel marker of cholangiolocellular carcinoma
Auteur(s) :
Hoshino, Hitomi [Auteur]
University of Fukui [Bunkyo]
Ohta, Makoto [Auteur]
University of Fukui [Bunkyo]
Ito, Makoto [Auteur]
Uchimura, Kenji [Auteur]
Nagoya University
Sakai, Yasuhiro [Auteur]
Shinshu University Hospital
Uehara, Takeshi [Auteur]
Shinshu University Hospital
Low, Shulin [Auteur]
University of Fukui [Bunkyo]
Fukushima, Mana [Auteur]
Shinshu University Hospital
Kobayashi, Motohiro [Auteur]
University of Fukui [Bunkyo]
University of Fukui [Bunkyo]
Ohta, Makoto [Auteur]
University of Fukui [Bunkyo]
Ito, Makoto [Auteur]
Uchimura, Kenji [Auteur]
Nagoya University
Sakai, Yasuhiro [Auteur]
Shinshu University Hospital
Uehara, Takeshi [Auteur]
Shinshu University Hospital
Low, Shulin [Auteur]
University of Fukui [Bunkyo]
Fukushima, Mana [Auteur]
Shinshu University Hospital
Kobayashi, Motohiro [Auteur]
University of Fukui [Bunkyo]
Titre de la revue :
Laboratory Investigation; a Journal of Technical Methods and Pathology
Nom court de la revue :
Lab. Invest.
Numéro :
96
Pagination :
1246-1255
Date de publication :
2016
ISSN :
1530-0307
Mot(s)-clé(s) en anglais :
Cell Membrane
Cell Polarity
Cricetinae
Cricetulus
Isoenzymes
Humans
Recombinant Proteins
Liver Neoplasms
Biomarkers, Tumor
Sulfuric Acid Esters
Protein Transport
Mice, Knockout
Mucin-1
Animals
Sulfotransferase
Cell Line, Tumor
Bile Duct Neoplasms
Antigens, Tumor-Associated, Carbohydrate
Bile Ducts, Intrahepatic
Membrane Protein
Antigens, Surface
Polysaccharide
Cholangiocarcinoma
CHO Cells
Cell Polarity
Cricetinae
Cricetulus
Isoenzymes
Humans
Recombinant Proteins
Liver Neoplasms
Biomarkers, Tumor
Sulfuric Acid Esters
Protein Transport
Mice, Knockout
Mucin-1
Animals
Sulfotransferase
Cell Line, Tumor
Bile Duct Neoplasms
Antigens, Tumor-Associated, Carbohydrate
Bile Ducts, Intrahepatic
Membrane Protein
Antigens, Surface
Polysaccharide
Cholangiocarcinoma
CHO Cells
Discipline(s) HAL :
Chimie/Chimie théorique et/ou physique
Résumé en anglais : [en]
Intrahepatic cholangiocarcinoma (ICC) is the second most common primary liver neoplasm, followed by hepatocellular carcinoma. ICC can be further subclassified as (i) perihilar and (ii) peripheral types, the latter ...
Lire la suite >Intrahepatic cholangiocarcinoma (ICC) is the second most common primary liver neoplasm, followed by hepatocellular carcinoma. ICC can be further subclassified as (i) perihilar and (ii) peripheral types, the latter histologically resembling small-sized intrahepatic bile ducts, such as interlobular bile ducts, cholangioles/ductules and the canals of Hering. Cholangiolocellular carcinoma (CoCC), now classified by the World Health Organization as a subtype of combined hepatocellular-cholangiocarcinoma, is currently regarded as a subtype of peripheral-type ICC. The present study was undertaken to determine whether sulfated glycans recognized by the MECA-79 monoclonal antibody could serve as a CoCC marker. Using immunohistochemistry, we show that MECA-79 sulfated glycans are preferentially expressed at the apical membrane of cholangiocytes found in small-sized intrahepatic bile ducts in normal liver and in canalicular structures formed in CoCC. We also report that apical membrane MECA-79 sulfated glycan expression colocalizes with that of mucin 1 (MUC1) core proteins. We also present immunoblotting of Chinese hamster ovary cells overexpressing FLAG-tagged MUC1 to show that MUC1 serves as a MECA-79 scaffold. Furthermore, we report that SSP-25 human ICC cells overexpressing N-acetylglucosamine-6-O-sulfotransferase 2 (GlcNAc6ST-2), but not GlcNAc6ST-1, exhibit membrane expression of MECA-79 sulfated glycans, suggesting that GlcNAc6ST-2 catalyzes MECA-79 epitope biosynthesis in cholangiocytes. Moreover, both wild-type and GlcNAc6ST-1 knockout mice exhibit apical membrane MECA-79 expression in small-sized intrahepatic bile ducts, namely interlobular bile ducts, whereas MECA-79 expression was completely absent in comparable tissues from GlcNAc6ST-1 and GlcNAc6ST-2 double knockout mice. These data collectively indicate that apical membrane localization of MUC1 proteins decorated with GlcNAc6ST-2-dependent MECA-79 sulfated glycans may mark cholangiocytes with cholangiolar/ductular differentiation and could serve as a useful CoCC marker.Lire moins >
Lire la suite >Intrahepatic cholangiocarcinoma (ICC) is the second most common primary liver neoplasm, followed by hepatocellular carcinoma. ICC can be further subclassified as (i) perihilar and (ii) peripheral types, the latter histologically resembling small-sized intrahepatic bile ducts, such as interlobular bile ducts, cholangioles/ductules and the canals of Hering. Cholangiolocellular carcinoma (CoCC), now classified by the World Health Organization as a subtype of combined hepatocellular-cholangiocarcinoma, is currently regarded as a subtype of peripheral-type ICC. The present study was undertaken to determine whether sulfated glycans recognized by the MECA-79 monoclonal antibody could serve as a CoCC marker. Using immunohistochemistry, we show that MECA-79 sulfated glycans are preferentially expressed at the apical membrane of cholangiocytes found in small-sized intrahepatic bile ducts in normal liver and in canalicular structures formed in CoCC. We also report that apical membrane MECA-79 sulfated glycan expression colocalizes with that of mucin 1 (MUC1) core proteins. We also present immunoblotting of Chinese hamster ovary cells overexpressing FLAG-tagged MUC1 to show that MUC1 serves as a MECA-79 scaffold. Furthermore, we report that SSP-25 human ICC cells overexpressing N-acetylglucosamine-6-O-sulfotransferase 2 (GlcNAc6ST-2), but not GlcNAc6ST-1, exhibit membrane expression of MECA-79 sulfated glycans, suggesting that GlcNAc6ST-2 catalyzes MECA-79 epitope biosynthesis in cholangiocytes. Moreover, both wild-type and GlcNAc6ST-1 knockout mice exhibit apical membrane MECA-79 expression in small-sized intrahepatic bile ducts, namely interlobular bile ducts, whereas MECA-79 expression was completely absent in comparable tissues from GlcNAc6ST-1 and GlcNAc6ST-2 double knockout mice. These data collectively indicate that apical membrane localization of MUC1 proteins decorated with GlcNAc6ST-2-dependent MECA-79 sulfated glycans may mark cholangiocytes with cholangiolar/ductular differentiation and could serve as a useful CoCC marker.Lire moins >
Langue :
Anglais
Audience :
Non spécifiée
Établissement(s) :
CNRS
Université de Lille
Université de Lille
Collections :
Date de dépôt :
2020-02-12T15:11:35Z
2021-03-25T08:41:12Z
2021-03-25T08:41:12Z