Keratan sulfate: biosynthesis, structures, ...
Type de document :
Article dans une revue scientifique
PMID :
URL permanente :
Titre :
Keratan sulfate: biosynthesis, structures, and biological functions
Auteur(s) :
Titre de la revue :
Methods in molecular biology (Clifton, N.J.)
Nom court de la revue :
Methods Mol. Biol.
Numéro :
1229
Pagination :
389-400
Date de publication :
2015
ISSN :
1940-6029
Mot(s)-clé(s) en anglais :
Immunohistochemistry
Brain
Animals
Mice, Inbred C57BL
Sulfotransferase
Oligosaccharide
Substrate Specificity
Cercopithecus aethiops
Epitopes
COS Cells
Keratan Sulfate
Brain
Animals
Mice, Inbred C57BL
Sulfotransferase
Oligosaccharide
Substrate Specificity
Cercopithecus aethiops
Epitopes
COS Cells
Keratan Sulfate
Discipline(s) HAL :
Chimie/Chimie théorique et/ou physique
Résumé en anglais : [en]
Keratan sulfate is a glycosaminoglycan that has been investigated in the cornea and skeletal tissues for decades. Endoglycosidases and monoclonal antibodies specific for keratan sulfate have been developed. These materials ...
Lire la suite >Keratan sulfate is a glycosaminoglycan that has been investigated in the cornea and skeletal tissues for decades. Endoglycosidases and monoclonal antibodies specific for keratan sulfate have been developed. These materials have facilitated the analysis of keratan sulfate biosynthesis and structures. Likewise, they have expedited study of the biological roles of keratan sulfate in vitro and in vivo. It has been shown that keratan sulfate is also expressed in the central nervous system and functions as a regulator of neuronal regeneration/sprouting. Here, we describe methods to determine the enzymatic activity of GlcNAc6ST, which is involved in keratan sulfate biosynthesis, and to extract and prepare ocular keratan sulfate for a disaccharide composition analysis. Immunohistochemistry for an anti-keratan sulfate epitope in the brain is also described.Lire moins >
Lire la suite >Keratan sulfate is a glycosaminoglycan that has been investigated in the cornea and skeletal tissues for decades. Endoglycosidases and monoclonal antibodies specific for keratan sulfate have been developed. These materials have facilitated the analysis of keratan sulfate biosynthesis and structures. Likewise, they have expedited study of the biological roles of keratan sulfate in vitro and in vivo. It has been shown that keratan sulfate is also expressed in the central nervous system and functions as a regulator of neuronal regeneration/sprouting. Here, we describe methods to determine the enzymatic activity of GlcNAc6ST, which is involved in keratan sulfate biosynthesis, and to extract and prepare ocular keratan sulfate for a disaccharide composition analysis. Immunohistochemistry for an anti-keratan sulfate epitope in the brain is also described.Lire moins >
Langue :
Anglais
Établissement(s) :
CNRS
Université de Lille
Université de Lille
Date de dépôt :
2020-02-12T15:11:37Z