Title :

KSGal6ST is essential for the 6-sulfation of galactose within keratan sulfate in early postnatal brain

Author(s) :

Hoshino, Hitomi [Auteur]
Foyez, Tahmina [Auteur]
Ohtake-Niimi, Shiori [Auteur]
Takeda-Uchimura, Yoshiko [Auteur]
Michikawa, Makoto [Auteur]
Kadomatsu, Kenji [Auteur]
Uchimura, Kenji [Auteur]

Journal title :

The Journal of Histochemistry and Cytochemistry. Official Journal of the Histochemistry Society

Abbreviated title :

J. Histochem. Cytochem.

Volume number :

62

Pages :

145-156

Publication date :

2014-02

ISSN :

1551-5044

English keyword(s) :

Animals, Newborn
Brain
Glycosaminoglycans
5D4
developing brain
Humans
Mice, Inbred C57BL
Mice, Knockout
Keratan Sulfate
Animals
Time Factors
Sulfotransferase
Phosphacan
Mice
HeLa Cells
Galactose
RNA, Messenger
extracellular matrix

HAL domain(s) :

Chimie/Chimie théorique et/ou physique

English abstract : [en]

Keratan sulfate (KS) comprises repeating disaccharides of galactose (Gal) and N-acetylglucosamine (GlcNAc). Residues of Gal and GlcNAc in KS are potentially modified with sulfate at their C-6 positions. The 5D4 monoclonal ...
Show more >Keratan sulfate (KS) comprises repeating disaccharides of galactose (Gal) and N-acetylglucosamine (GlcNAc). Residues of Gal and GlcNAc in KS are potentially modified with sulfate at their C-6 positions. The 5D4 monoclonal antibody recognizes KS structures containing Gal and GlcNAc, both 6-sulfated, and has been used most extensively to evaluate KS expression in mammalian brains. We previously showed that GlcNAc6ST1 is an enzyme responsible for the synthesis of the 5D4 epitope in developing brain and in the adult brain, where it is induced after injury. It has been unclear which sulfotransferase is responsible for Gal-6-sulfation within the 5D4 KS epitope in developing brains. We produced mice deficient in KSGal6ST, a Gal-6-sulfotransferase. Western blotting and immunoprecipitation revealed that all 5D4-immunoreactivity to proteins, including phosphacan, were abolished in KSGal6ST-deficient postnatal brains. Likewise, the 5D4 epitope, expressed primarily in the cortical marginal zone and subplate and dorsal thalamus, was eliminated in KSGal6ST-deficient mice. Disaccharide analysis showed the loss of Gal-6-sulfate in KS of the KSGal6ST-deficient brains. Transfection studies revealed that GlcNAc6ST1 and KSGal6ST cooperated in the expression of the 5D4 KS epitope in HeLa cells. These results indicate that KSGal6ST is essential for C-6 sulfation of Gal within KS in early postnatal brains.Show less >

Language :

Anglais

Administrative institution(s) :

CNRS
Université de Lille

Collections :

• Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576

Submission date :

2020-02-12T15:11:39Z