Functions of maize genes encoding pyruvate ...
Type de document :
Article dans une revue scientifique
DOI :
PMID :
URL permanente :
Titre :
Functions of maize genes encoding pyruvate phosphate dikinase in developing endosperm
Auteur(s) :
Lappe, Ryan R. [Auteur]
Iowa State University [ISU]
Baier, John W. [Auteur]
University of Florida [Gainesville] [UF]
Boehlein, Susan K. [Auteur]
University of Florida [Gainesville] [UF]
Huffman, Ryan [Auteur]
Iowa State University [ISU]
Lin, Qiaohui [Auteur]
Iowa State University [ISU]
Wattebled, Fabrice [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Settles, A. Mark [Auteur]
University of Florida [Gainesville] [UF]
Hannah, L. Curtis [Auteur]
University of Florida [Gainesville] [UF]
Borisjuk, Ljudmilla [Auteur]
Leibniz Institute of Plant Genetics and Crop Plant Research [Gatersleben] [IPK-Gatersleben]
Rolletschek, Hardy [Auteur]
Leibniz Institute of Plant Genetics and Crop Plant Research [Gatersleben] [IPK-Gatersleben]
Stewart, Jon D. [Auteur]
University of Florida [Gainesville] [UF]
Scott, M. Paul [Auteur]
Iowa State University [ISU]
Hennen-Bierwagen, Tracie A. [Auteur]
Iowa State University [ISU]
Myers, Alan M. [Auteur]
Iowa State University [ISU]
Iowa State University [ISU]
Baier, John W. [Auteur]
University of Florida [Gainesville] [UF]
Boehlein, Susan K. [Auteur]
University of Florida [Gainesville] [UF]
Huffman, Ryan [Auteur]
Iowa State University [ISU]
Lin, Qiaohui [Auteur]
Iowa State University [ISU]
Wattebled, Fabrice [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Settles, A. Mark [Auteur]
University of Florida [Gainesville] [UF]
Hannah, L. Curtis [Auteur]
University of Florida [Gainesville] [UF]
Borisjuk, Ljudmilla [Auteur]
Leibniz Institute of Plant Genetics and Crop Plant Research [Gatersleben] [IPK-Gatersleben]
Rolletschek, Hardy [Auteur]
Leibniz Institute of Plant Genetics and Crop Plant Research [Gatersleben] [IPK-Gatersleben]
Stewart, Jon D. [Auteur]
University of Florida [Gainesville] [UF]
Scott, M. Paul [Auteur]
Iowa State University [ISU]
Hennen-Bierwagen, Tracie A. [Auteur]
Iowa State University [ISU]
Myers, Alan M. [Auteur]
Iowa State University [ISU]
Titre de la revue :
Proceedings of the National Academy of Sciences of the United States of America
Nom court de la revue :
Proc. Natl. Acad. Sci. U.S.A.
Numéro :
115
Pagination :
E24-E33
Date de publication :
2018-01
ISSN :
1091-6490
Mot(s)-clé(s) en anglais :
Pyruvate, Orthophosphate Dikinase
PPDK
opaque
Zea mays
Energy Metabolism
Starch
Transcription Factors
Endosperm
metabolism
Plant Proteins
Mutation
maize
PPDK
opaque
Zea mays
Energy Metabolism
Starch
Transcription Factors
Endosperm
metabolism
Plant Proteins
Mutation
maize
Discipline(s) HAL :
Chimie/Chimie théorique et/ou physique
Sciences du Vivant [q-bio]/Biologie végétale
Sciences du Vivant [q-bio]/Biologie végétale
Résumé en anglais : [en]
Maize opaque2 (o2) mutations are beneficial for endosperm nutritional quality but cause negative pleiotropic effects for reasons that are not fully understood. Direct targets of the bZIP transcriptional regulator encoded ...
Lire la suite >Maize opaque2 (o2) mutations are beneficial for endosperm nutritional quality but cause negative pleiotropic effects for reasons that are not fully understood. Direct targets of the bZIP transcriptional regulator encoded by o2 include pdk1 and pdk2 that specify pyruvate phosphate dikinase (PPDK). This enzyme reversibly converts AMP, pyrophosphate, and phosphoenolpyruvate to ATP, orthophosphate, and pyruvate and provides diverse functions in plants. This study addressed PPDK function in maize starchy endosperm where it is highly abundant during grain fill. pdk1 and pdk2 were inactivated individually by transposon insertions, and both genes were simultaneously targeted by endosperm-specific RNAi. pdk2 accounts for the large majority of endosperm PPDK, whereas pdk1 specifies the abundant mesophyll form. The pdk1- mutation is seedling-lethal, indicating that C4 photosynthesis is essential in maize. RNAi expression in transgenic endosperm eliminated detectable PPDK protein and enzyme activity. Transgenic kernels weighed the same on average as nontransgenic siblings, with normal endosperm starch and total N contents, indicating that PPDK is not required for net storage compound synthesis. An opaque phenotype resulted from complete PPDK knockout, including loss of vitreous endosperm character similar to the phenotype conditioned by o2-. Concentrations of multiple glycolytic intermediates were elevated in transgenic endosperm, energy charge was altered, and starch granules were more numerous but smaller on average than normal. The data indicate that PPDK modulates endosperm metabolism, potentially through reversible adjustments to energy charge, and reveal that o2- mutations can affect the opaque phenotype through regulation of PPDK in addition to their previously demonstrated effects on storage protein gene expression.Lire moins >
Lire la suite >Maize opaque2 (o2) mutations are beneficial for endosperm nutritional quality but cause negative pleiotropic effects for reasons that are not fully understood. Direct targets of the bZIP transcriptional regulator encoded by o2 include pdk1 and pdk2 that specify pyruvate phosphate dikinase (PPDK). This enzyme reversibly converts AMP, pyrophosphate, and phosphoenolpyruvate to ATP, orthophosphate, and pyruvate and provides diverse functions in plants. This study addressed PPDK function in maize starchy endosperm where it is highly abundant during grain fill. pdk1 and pdk2 were inactivated individually by transposon insertions, and both genes were simultaneously targeted by endosperm-specific RNAi. pdk2 accounts for the large majority of endosperm PPDK, whereas pdk1 specifies the abundant mesophyll form. The pdk1- mutation is seedling-lethal, indicating that C4 photosynthesis is essential in maize. RNAi expression in transgenic endosperm eliminated detectable PPDK protein and enzyme activity. Transgenic kernels weighed the same on average as nontransgenic siblings, with normal endosperm starch and total N contents, indicating that PPDK is not required for net storage compound synthesis. An opaque phenotype resulted from complete PPDK knockout, including loss of vitreous endosperm character similar to the phenotype conditioned by o2-. Concentrations of multiple glycolytic intermediates were elevated in transgenic endosperm, energy charge was altered, and starch granules were more numerous but smaller on average than normal. The data indicate that PPDK modulates endosperm metabolism, potentially through reversible adjustments to energy charge, and reveal that o2- mutations can affect the opaque phenotype through regulation of PPDK in addition to their previously demonstrated effects on storage protein gene expression.Lire moins >
Langue :
Anglais
Audience :
Non spécifiée
Établissement(s) :
CNRS
Université de Lille
Université de Lille
Équipe(s) de recherche :
Plant Storage Polysaccharides
Date de dépôt :
2020-02-12T15:11:42Z
2021-03-04T09:44:05Z
2021-03-04T09:44:05Z