Expression of Escherichia coli glycogen ...
Type de document :
Article dans une revue scientifique
DOI :
PMID :
URL permanente :
Titre :
Expression of Escherichia coli glycogen branching enzyme in an Arabidopsis mutant devoid of endogenous starch branching enzymes induces the synthesis of starch-like polyglucans
Auteur(s) :
Boyer, Laura [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Roussel, Xavier [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576
Courseaux, Adeline [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Ndjindji, Ofilia M. [Auteur]
Centre de Recherches sur les Macromolécules Végétales [CERMAV]
Lancelon-Pin, Christine [Auteur]
Centre de Recherches sur les Macromolécules Végétales [CERMAV]
Putaux, Jean-Luc [Auteur]
Centre de Recherches sur les Macromolécules Végétales [CERMAV]
Tetlow, Ian J. [Auteur]
University of Guelph
Emes, Michael J. [Auteur]
University of Guelph
Pontoire, Bruno [Auteur]
Unité de recherche sur les Biopolymères, Interactions Assemblages [BIA]
D'hulst, Christophe [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576
Wattebled, Fabrice [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Roussel, Xavier [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576
Courseaux, Adeline [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Ndjindji, Ofilia M. [Auteur]
Centre de Recherches sur les Macromolécules Végétales [CERMAV]
Lancelon-Pin, Christine [Auteur]
Centre de Recherches sur les Macromolécules Végétales [CERMAV]
Putaux, Jean-Luc [Auteur]
Centre de Recherches sur les Macromolécules Végétales [CERMAV]
Tetlow, Ian J. [Auteur]
University of Guelph
Emes, Michael J. [Auteur]
University of Guelph
Pontoire, Bruno [Auteur]
Unité de recherche sur les Biopolymères, Interactions Assemblages [BIA]
D'hulst, Christophe [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576
Wattebled, Fabrice [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576
Titre de la revue :
Plant, Cell & Environment
Nom court de la revue :
Plant Cell Environ.
Numéro :
39
Pagination :
1432-1447
Date de publication :
2016
ISSN :
1365-3040
Mot(s)-clé(s) en anglais :
Arabidopsis thaliana
GlgB
Chloroplasts
Arabidopsis
Escherichia coli
branching enzyme
Glucans
Plants, Genetically Modified
α-glucan
Carbohydrate Metabolism
1,4-alpha-Glucan Branching Enzyme
Debranching Enzyme
GlgB
Chloroplasts
Arabidopsis
Escherichia coli
branching enzyme
Glucans
Plants, Genetically Modified
α-glucan
Carbohydrate Metabolism
1,4-alpha-Glucan Branching Enzyme
Debranching Enzyme
Discipline(s) HAL :
Chimie/Chimie théorique et/ou physique
Sciences du Vivant [q-bio]/Biologie végétale
Sciences du Vivant [q-bio]/Biologie végétale
Résumé en anglais : [en]
Starch synthesis requires several enzymatic activities including branching enzymes (BEs) responsible for the formation of α(1 → 6) linkages. Distribution and number of these linkages are further controlled by debranching ...
Lire la suite >Starch synthesis requires several enzymatic activities including branching enzymes (BEs) responsible for the formation of α(1 → 6) linkages. Distribution and number of these linkages are further controlled by debranching enzymes that cleave some of them, rendering the polyglucan water-insoluble and semi-crystalline. Although the activity of BEs and debranching enzymes is mandatory to sustain normal starch synthesis, the relative importance of each in the establishment of the plant storage polyglucan (i.e. water insolubility, crystallinity and presence of amylose) is still debated. Here, we have substituted the activity of BEs in Arabidopsis with that of the Escherichia coli glycogen BE (GlgB). The latter is the BE counterpart in the metabolism of glycogen, a highly branched water-soluble and amorphous storage polyglucan. GlgB was expressed in the be2 be3 double mutant of Arabidopsis, which is devoid of BE activity and consequently free of starch. The synthesis of a water-insoluble, partly crystalline, amylose-containing starch-like polyglucan was restored in GlgB-expressing plants, suggesting that BEs' origin only has a limited impact on establishing essential characteristics of starch. Moreover, the balance between branching and debranching is crucial for the synthesis of starch, as an excess of branching activity results in the formation of highly branched, water-soluble, poorly crystalline polyglucan.Lire moins >
Lire la suite >Starch synthesis requires several enzymatic activities including branching enzymes (BEs) responsible for the formation of α(1 → 6) linkages. Distribution and number of these linkages are further controlled by debranching enzymes that cleave some of them, rendering the polyglucan water-insoluble and semi-crystalline. Although the activity of BEs and debranching enzymes is mandatory to sustain normal starch synthesis, the relative importance of each in the establishment of the plant storage polyglucan (i.e. water insolubility, crystallinity and presence of amylose) is still debated. Here, we have substituted the activity of BEs in Arabidopsis with that of the Escherichia coli glycogen BE (GlgB). The latter is the BE counterpart in the metabolism of glycogen, a highly branched water-soluble and amorphous storage polyglucan. GlgB was expressed in the be2 be3 double mutant of Arabidopsis, which is devoid of BE activity and consequently free of starch. The synthesis of a water-insoluble, partly crystalline, amylose-containing starch-like polyglucan was restored in GlgB-expressing plants, suggesting that BEs' origin only has a limited impact on establishing essential characteristics of starch. Moreover, the balance between branching and debranching is crucial for the synthesis of starch, as an excess of branching activity results in the formation of highly branched, water-soluble, poorly crystalline polyglucan.Lire moins >
Langue :
Anglais
Audience :
Non spécifiée
Établissement(s) :
CNRS
Université de Lille
Université de Lille
Équipe(s) de recherche :
Plant Storage Polysaccharides
Date de dépôt :
2020-02-12T15:11:43Z
2021-03-03T09:59:36Z
2021-03-03T09:59:36Z