Hyper-accumulation of starch and oil in a ...
Type de document :
Article dans une revue scientifique
PMID :
URL permanente :
Titre :
Hyper-accumulation of starch and oil in a Chlamydomonas mutant affected in a plant-specific DYRK kinase
Auteur(s) :
Schulz-Raffelt, Miriam [Auteur]
Institut de Biosciences et Biotechnologies d'Aix-Marseille (ex-IBEB) [BIAM]
Chochois, Vincent [Auteur]
Institut de Biosciences et Biotechnologies d'Aix-Marseille (ex-IBEB) [BIAM]
Auroy, Pascaline [Auteur]
Institut de Biosciences et Biotechnologies d'Aix-Marseille (ex-IBEB) [BIAM]
Cuiné, Stéphan [Auteur]
Institut de Biosciences et Biotechnologies d'Aix-Marseille (ex-IBEB) [BIAM]
Billon, Emmanuelle [Auteur]
Institut de Biosciences et Biotechnologies d'Aix-Marseille (ex-IBEB) [BIAM]
Dauvillee, David [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Li-Beisson, Yonghua [Auteur]
Institut de Biosciences et Biotechnologies d'Aix-Marseille (ex-IBEB) [BIAM]
Peltier, Gilles [Auteur]
Institut de Biosciences et Biotechnologies d'Aix-Marseille (ex-IBEB) [BIAM]
Institut de Biosciences et Biotechnologies d'Aix-Marseille (ex-IBEB) [BIAM]
Chochois, Vincent [Auteur]
Institut de Biosciences et Biotechnologies d'Aix-Marseille (ex-IBEB) [BIAM]
Auroy, Pascaline [Auteur]
Institut de Biosciences et Biotechnologies d'Aix-Marseille (ex-IBEB) [BIAM]
Cuiné, Stéphan [Auteur]
Institut de Biosciences et Biotechnologies d'Aix-Marseille (ex-IBEB) [BIAM]
Billon, Emmanuelle [Auteur]
Institut de Biosciences et Biotechnologies d'Aix-Marseille (ex-IBEB) [BIAM]
Dauvillee, David [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Li-Beisson, Yonghua [Auteur]
Institut de Biosciences et Biotechnologies d'Aix-Marseille (ex-IBEB) [BIAM]
Peltier, Gilles [Auteur]
Institut de Biosciences et Biotechnologies d'Aix-Marseille (ex-IBEB) [BIAM]
Titre de la revue :
Biotechnology for Biofuels
Nom court de la revue :
Biotechnol Biofuels
Numéro :
9
Pagination :
55
Date de publication :
2016
ISSN :
1754-6834
Mot(s)-clé(s) en anglais :
Nutrient deprivation
Oil
Starch
Kinase
Microalgae
Photosynthesis
Chlamydomonas
DYRK
Oil
Starch
Kinase
Microalgae
Photosynthesis
Chlamydomonas
DYRK
Discipline(s) HAL :
Chimie/Chimie théorique et/ou physique
Sciences du Vivant [q-bio]/Biologie végétale
Sciences du Vivant [q-bio]/Biologie végétale
Résumé en anglais : [en]
BACKGROUND: Because of their high biomass productivity and their ability to accumulate high levels of energy-rich reserve compounds such as oils or starch, microalgae represent a promising feedstock for the production of ...
Lire la suite >BACKGROUND: Because of their high biomass productivity and their ability to accumulate high levels of energy-rich reserve compounds such as oils or starch, microalgae represent a promising feedstock for the production of biofuel. Accumulation of reserve compounds takes place when microalgae face adverse situations such as nutrient shortage, conditions which also provoke a stop in cell division, and down-regulation of photosynthesis. Despite growing interest in microalgal biofuels, little is known about molecular mechanisms controlling carbon reserve formation. In order to discover new regulatory mechanisms, and identify genes of interest to boost the potential of microalgae for biofuel production, we developed a forward genetic approach in the model microalga Chlamydomonas reinhardtii. RESULTS: By screening an insertional mutant library on the ability of mutants to accumulate and re-mobilize reserve compounds, we isolated a Chlamydomonas mutant (starch degradation 1, std1) deficient for a dual-specificity tyrosine-phosphorylation-regulated kinase (DYRK). The std1 mutant accumulates higher levels of starch and oil than wild-type and maintains a higher photosynthetic activity under nitrogen starvation. Phylogenetic analysis revealed that this kinase (named DYRKP) belongs to a plant-specific subgroup of the evolutionarily conserved DYRK kinase family. Furthermore, hyper-accumulation of storage compounds occurs in std1 mostly under low light in photoautotrophic condition, suggesting that the kinase normally acts under conditions of low energy status to limit reserve accumulation. CONCLUSIONS: The DYRKP kinase is proposed to act as a negative regulator of the sink capacity of photosynthetic cells that integrates nutrient and energy signals. Inactivation of the kinase strongly boosts accumulation of reserve compounds under photoautotrophic nitrogen deprivation and allows maintaining high photosynthetic activity. The DYRKP kinase therefore represents an attractive target for improving the energy density of microalgae or crop plants.Lire moins >
Lire la suite >BACKGROUND: Because of their high biomass productivity and their ability to accumulate high levels of energy-rich reserve compounds such as oils or starch, microalgae represent a promising feedstock for the production of biofuel. Accumulation of reserve compounds takes place when microalgae face adverse situations such as nutrient shortage, conditions which also provoke a stop in cell division, and down-regulation of photosynthesis. Despite growing interest in microalgal biofuels, little is known about molecular mechanisms controlling carbon reserve formation. In order to discover new regulatory mechanisms, and identify genes of interest to boost the potential of microalgae for biofuel production, we developed a forward genetic approach in the model microalga Chlamydomonas reinhardtii. RESULTS: By screening an insertional mutant library on the ability of mutants to accumulate and re-mobilize reserve compounds, we isolated a Chlamydomonas mutant (starch degradation 1, std1) deficient for a dual-specificity tyrosine-phosphorylation-regulated kinase (DYRK). The std1 mutant accumulates higher levels of starch and oil than wild-type and maintains a higher photosynthetic activity under nitrogen starvation. Phylogenetic analysis revealed that this kinase (named DYRKP) belongs to a plant-specific subgroup of the evolutionarily conserved DYRK kinase family. Furthermore, hyper-accumulation of storage compounds occurs in std1 mostly under low light in photoautotrophic condition, suggesting that the kinase normally acts under conditions of low energy status to limit reserve accumulation. CONCLUSIONS: The DYRKP kinase is proposed to act as a negative regulator of the sink capacity of photosynthetic cells that integrates nutrient and energy signals. Inactivation of the kinase strongly boosts accumulation of reserve compounds under photoautotrophic nitrogen deprivation and allows maintaining high photosynthetic activity. The DYRKP kinase therefore represents an attractive target for improving the energy density of microalgae or crop plants.Lire moins >
Langue :
Anglais
Audience :
Non spécifiée
Établissement(s) :
CNRS
Université de Lille
Université de Lille
Équipe(s) de recherche :
Plant Storage Polysaccharides
Date de dépôt :
2020-02-12T15:11:43Z
2021-03-04T13:18:35Z
2021-03-04T13:18:35Z
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