Title :

Evaluation of novel starch-deficient mutants of Chlorella sorokiniana for hyper-accumulation of lipids

Author(s) :

Vonlanthen, Sofie [Auteur]
University College of London [London] [UCL]
Dauvillee, David [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 [UGSF]
Purton, Saul [Auteur]
University College of London [London] [UCL]

Journal title :

Algal Research

Abbreviated title :

Algal Res

Volume number :

12

Pages :

109-118

Publication date :

2015-11

ISSN :

2211-9264

English keyword(s) :

Microalgae
Biofuel
Chlorella
Starch
Isoamylase
Triacylglycerides

HAL domain(s) :

Chimie/Chimie théorique et/ou physique

English abstract : [en]

When green algae are exposed to physiological stresses such as nutrient deprivation, growth is arrested and the cells channel fixed carbon instead into storage compounds, accumulating first starch granules and then lipid ...
Show more >When green algae are exposed to physiological stresses such as nutrient deprivation, growth is arrested and the cells channel fixed carbon instead into storage compounds, accumulating first starch granules and then lipid bodies containing triacylglycerides. In recent years there has been significant interest in the commercial exploitation of algal lipids as a sustainable source of biodiesel. Since starch and lipid biosynthesis involves the same C3 precursor pool, it has been proposed that mutations blocking starch accumulation should result in increased lipid yields, and indeed several studies have supported this. The fast-growing, thermotolerant alga Chlorella sorokiniana represents an attractive strain for industrial cultivation. We have therefore generated and characterized starch-deficient mutants of C. sorokiniana and determined whether lipid levels are increased in these strains under stress conditions. One mutant (ST68) is shown to lack isoamylase, whilst two others (ST3 and ST12) are defective in starch phosphorylase. However, we find no significant change in the accumulation or profile of fatty acids in these mutants compared to the wild-type, suggesting that a failure to accumulate starch per se is not sufficient for the hyper-accumulation of lipid, and that more subtle regulatory steps underlie the partitioning of carbon to the two storage products.Show less >

Language :

Anglais

Audience :

Non spécifiée

Administrative institution(s) :

CNRS
Université de Lille

Collections :

• Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576

Research team(s) :

Plant Storage Polysaccharides

Submission date :

2020-02-12T15:11:43Z
2021-05-07T11:54:58Z