Tracking sulfur and phosphorus within ...
Type de document :
Article dans une revue scientifique
PMID :
URL permanente :
Titre :
Tracking sulfur and phosphorus within single starch granules using synchrotron X-ray microfluorescence mapping
Auteur(s) :
Buléon, Alain [Auteur]
Cotte, Marine [Auteur]
Putaux, Jean-Luc [Auteur]
D'hulst, Christophe [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576
Susini, Jean [Auteur]
Cotte, Marine [Auteur]
Putaux, Jean-Luc [Auteur]
D'hulst, Christophe [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576
Susini, Jean [Auteur]
Titre de la revue :
Biochimica et biophysica acta
Nom court de la revue :
Biochim. Biophys. Acta
Numéro :
1840
Pagination :
113-119
Date de publication :
2014-01
ISSN :
0006-3002
Mot(s)-clé(s) en anglais :
Triticum
Cytoplasmic Granules
Micro X-ray fluorescence
Starch Synthase
Solanum tuberosum
Starch
Granule bound starch synthase
Spectrometry, X-Ray Emission
Phosphorus
Sulfur
Synchrotron
Cytoplasmic Granules
Micro X-ray fluorescence
Starch Synthase
Solanum tuberosum
Starch
Granule bound starch synthase
Spectrometry, X-Ray Emission
Phosphorus
Sulfur
Synchrotron
Discipline(s) HAL :
Chimie/Chimie théorique et/ou physique
Résumé en anglais : [en]
BACKGROUND: Native starch accumulates as granules containing two glucose polymers: amylose and amylopectin. Phosphate (0.2-0.5%) and proteins (0.1-0.7%) are also present in some starches. Phosphate groups play a major role ...
Lire la suite >BACKGROUND: Native starch accumulates as granules containing two glucose polymers: amylose and amylopectin. Phosphate (0.2-0.5%) and proteins (0.1-0.7%) are also present in some starches. Phosphate groups play a major role in starch metabolism while granule-bound starch synthase 1 (GBSS1) which represents up to 95% of the proteins bound to the granule is responsible for amylose biosynthesis. METHODS: Synchrotron micro-X-ray fluorescence (μXRF) was used for the first time for high-resolution mapping of GBSS1 and phosphate groups based on the XRF signal of sulfur (S) and phosphorus (P), respectively. Wild-type starches were studied as well as their related mutants lacking GBSS1 or starch-phosphorylating enzyme. RESULTS: Wild-type potato and maize starch exhibited high level of phosphorylation and high content of sulfur respectively when compared to mutant potato starch lacking glucan water dikinase (GWD) and mutant maize starch lacking GBSS1. Phosphate groups are mostly present at the periphery of wild-type potato starch granules, and spread all over the granule in the amylose-free mutant. P and S XRF were also measured within single small starch granules from Arabidopsis or Chlamydomonas not exceeding 3-5μm in diameter. CONCLUSIONS: Imaging GBSS1 (by S mapping) in potato starch sections showed that the antisense technique suppresses the expression of GBSS1 during biosynthesis. P mapping confirmed that amylose is mostly present in the center of the granule, which had been suggested before. GENERAL SIGNIFICANCE: μXRF is a potentially powerful technique to analyze the minor constituents of starch and understand starch structure/properties or biosynthesis by the use of selected genetic backgrounds.Lire moins >
Lire la suite >BACKGROUND: Native starch accumulates as granules containing two glucose polymers: amylose and amylopectin. Phosphate (0.2-0.5%) and proteins (0.1-0.7%) are also present in some starches. Phosphate groups play a major role in starch metabolism while granule-bound starch synthase 1 (GBSS1) which represents up to 95% of the proteins bound to the granule is responsible for amylose biosynthesis. METHODS: Synchrotron micro-X-ray fluorescence (μXRF) was used for the first time for high-resolution mapping of GBSS1 and phosphate groups based on the XRF signal of sulfur (S) and phosphorus (P), respectively. Wild-type starches were studied as well as their related mutants lacking GBSS1 or starch-phosphorylating enzyme. RESULTS: Wild-type potato and maize starch exhibited high level of phosphorylation and high content of sulfur respectively when compared to mutant potato starch lacking glucan water dikinase (GWD) and mutant maize starch lacking GBSS1. Phosphate groups are mostly present at the periphery of wild-type potato starch granules, and spread all over the granule in the amylose-free mutant. P and S XRF were also measured within single small starch granules from Arabidopsis or Chlamydomonas not exceeding 3-5μm in diameter. CONCLUSIONS: Imaging GBSS1 (by S mapping) in potato starch sections showed that the antisense technique suppresses the expression of GBSS1 during biosynthesis. P mapping confirmed that amylose is mostly present in the center of the granule, which had been suggested before. GENERAL SIGNIFICANCE: μXRF is a potentially powerful technique to analyze the minor constituents of starch and understand starch structure/properties or biosynthesis by the use of selected genetic backgrounds.Lire moins >
Langue :
Anglais
Établissement(s) :
CNRS
Université de Lille
Université de Lille
Équipe(s) de recherche :
Plant Storage Polysaccharides
Date de dépôt :
2020-02-12T15:11:44Z