In vitro synthesis of hyperbranched α-glucans ...
Type de document :
Article dans une revue scientifique
DOI :
PMID :
URL permanente :
Titre :
In vitro synthesis of hyperbranched α-glucans using a biomimetic enzymatic toolbox
Auteur(s) :
Grimaud, Florent [Auteur]
Lancelon-Pin, Christine [Auteur]
Rolland-Sabaté, Agnès [Auteur]
Roussel, Xavier [Auteur]
Laguerre, Sandrine [Auteur]
Viksø-Nielsen, Anders [Auteur]
Putaux, Jean-Luc [Auteur]
Guilois, Sophie [Auteur]
Buléon, Alain [Auteur]
D'hulst, Christophe [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576
Potocki-Véronèse, Gabrielle [Auteur]
Lancelon-Pin, Christine [Auteur]
Rolland-Sabaté, Agnès [Auteur]
Roussel, Xavier [Auteur]
Laguerre, Sandrine [Auteur]
Viksø-Nielsen, Anders [Auteur]
Putaux, Jean-Luc [Auteur]
Guilois, Sophie [Auteur]
Buléon, Alain [Auteur]
D'hulst, Christophe [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576
Potocki-Véronèse, Gabrielle [Auteur]
Titre de la revue :
Biomacromolecules
Nom court de la revue :
Biomacromolecules
Numéro :
14
Pagination :
438-447
Date de publication :
2013-02-11
ISSN :
1526-4602
Mot(s)-clé(s) en anglais :
Rhodothermus
Biomimetics
Starch
Glycosyltransferase
Glucans
Neisseria
1,4-alpha-Glucan Branching Enzyme
Glutathione Transferase
Biomimetics
Starch
Glycosyltransferase
Glucans
Neisseria
1,4-alpha-Glucan Branching Enzyme
Glutathione Transferase
Discipline(s) HAL :
Chimie/Chimie théorique et/ou physique
Résumé en anglais : [en]
Glycogen biosynthesis requires the coordinated action of elongating and branching enzymes, of which the synergetic action is still not clearly understood. We have designed an experimental plan to develop and fully exploit ...
Lire la suite >Glycogen biosynthesis requires the coordinated action of elongating and branching enzymes, of which the synergetic action is still not clearly understood. We have designed an experimental plan to develop and fully exploit a biomimetic system reproducing in vitro the activities involved in the formation of α(1,4) and α(1,6) glycosidic linkages during glycogen biosynthesis. This method is based on the use of two bacterial transglucosidases, the amylosucrase from Neisseria polysaccharea and the branching enzyme from Rhodothermus obamensis . The α-glucans synthesized from sucrose, a low cost agroresource, by the tandem action of the two enzymes, have been characterized by using complementary enzymatic, chromatographic, and imaging techniques. In a single step, linear and branched α-glucans were obtained, whose proportions, morphology, molar mass, and branching degree depended on both the initial sucrose concentration and the ratio between elongating and branching enzymes. In particular, spherical hyperbranched α-glucans with a controlled mean diameter (ranging from 10 to 150 nm), branching degree (from 10 to 13%), and weight-average molar mass (3.7 × 10(6) to 4.4 × 10(7) g.mol(-1)) were synthesized. Despite their structure, which is similar to that of natural glycogens, the mechanisms involved in their in vitro synthesis appeared to be different from those involved in the biosynthesis of native hyperbranched α-glucans.Lire moins >
Lire la suite >Glycogen biosynthesis requires the coordinated action of elongating and branching enzymes, of which the synergetic action is still not clearly understood. We have designed an experimental plan to develop and fully exploit a biomimetic system reproducing in vitro the activities involved in the formation of α(1,4) and α(1,6) glycosidic linkages during glycogen biosynthesis. This method is based on the use of two bacterial transglucosidases, the amylosucrase from Neisseria polysaccharea and the branching enzyme from Rhodothermus obamensis . The α-glucans synthesized from sucrose, a low cost agroresource, by the tandem action of the two enzymes, have been characterized by using complementary enzymatic, chromatographic, and imaging techniques. In a single step, linear and branched α-glucans were obtained, whose proportions, morphology, molar mass, and branching degree depended on both the initial sucrose concentration and the ratio between elongating and branching enzymes. In particular, spherical hyperbranched α-glucans with a controlled mean diameter (ranging from 10 to 150 nm), branching degree (from 10 to 13%), and weight-average molar mass (3.7 × 10(6) to 4.4 × 10(7) g.mol(-1)) were synthesized. Despite their structure, which is similar to that of natural glycogens, the mechanisms involved in their in vitro synthesis appeared to be different from those involved in the biosynthesis of native hyperbranched α-glucans.Lire moins >
Langue :
Anglais
Établissement(s) :
CNRS
Université de Lille
Université de Lille
Équipe(s) de recherche :
Plant Storage Polysaccharides
Date de dépôt :
2020-02-12T15:11:46Z