Correction of spherical aberration in ...
Document type :
Article dans une revue scientifique
DOI :
PMID :
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Title :
Correction of spherical aberration in multi-focal multiphoton microscopy with spatial light modulator
Author(s) :
Matsumoto, Naoya [Auteur]
Konno, Alu [Auteur]
Ohbayashi, Yasushi [Auteur]
Inoue, Takashi [Auteur]
Matsumoto, Akiyuki [Auteur]
Nagoya University
Uchimura, Kenji [Auteur]
Nagoya University
Kadomatsu, Kenji [Auteur]
Nagoya University
Okazaki, Shigetoshi [Auteur]
Konno, Alu [Auteur]
Ohbayashi, Yasushi [Auteur]
Inoue, Takashi [Auteur]
Matsumoto, Akiyuki [Auteur]
Nagoya University
Uchimura, Kenji [Auteur]
Nagoya University
Kadomatsu, Kenji [Auteur]
Nagoya University
Okazaki, Shigetoshi [Auteur]
Journal title :
Optics Express
Abbreviated title :
Opt Express
Volume number :
25
Pages :
7055-7068
Publication date :
2017-03-20
ISSN :
1094-4087
English keyword(s) :
Blood Vessels
Animals
Image Enhancement
Light
Refractometry
Mice
Microscopy, Fluorescence, Multiphoton
Animals
Image Enhancement
Light
Refractometry
Mice
Microscopy, Fluorescence, Multiphoton
HAL domain(s) :
Chimie/Chimie théorique et/ou physique
English abstract : [en]
We demonstrate that high-quality images of the deep regions of a thick sample can be obtained from its surface by multi-focal multiphoton microscopy (MMM). The MMM system incorporates a spatial light modulator to separate ...
Show more >We demonstrate that high-quality images of the deep regions of a thick sample can be obtained from its surface by multi-focal multiphoton microscopy (MMM). The MMM system incorporates a spatial light modulator to separate the excitation beam into a multi-focal excitation beam and modulate the pre-distortion wavefront to correct spherical aberration (SA) caused by a refractive index mismatch between the immersion medium and the biological sample. When fluorescent beads in transparent epoxy resin were observed using four SA-corrected focal beams, the fluorescence signal of the obtained images was ~52 times higher than that obtained without SA correction until a depth of ~1100 μm, similar to the result for single-focal multiphoton microscopy (SMM). The MMM scanning time was four times less than that for SMM, and MMM showed an improved fluorescence intensity and depth resolution for an image of blood vessels in the brain of a mouse stained with a fluorescent dye.Show less >
Show more >We demonstrate that high-quality images of the deep regions of a thick sample can be obtained from its surface by multi-focal multiphoton microscopy (MMM). The MMM system incorporates a spatial light modulator to separate the excitation beam into a multi-focal excitation beam and modulate the pre-distortion wavefront to correct spherical aberration (SA) caused by a refractive index mismatch between the immersion medium and the biological sample. When fluorescent beads in transparent epoxy resin were observed using four SA-corrected focal beams, the fluorescence signal of the obtained images was ~52 times higher than that obtained without SA correction until a depth of ~1100 μm, similar to the result for single-focal multiphoton microscopy (SMM). The MMM scanning time was four times less than that for SMM, and MMM showed an improved fluorescence intensity and depth resolution for an image of blood vessels in the brain of a mouse stained with a fluorescent dye.Show less >
Language :
Anglais
Audience :
Non spécifiée
Administrative institution(s) :
CNRS
Université de Lille
Université de Lille
Collections :
Submission date :
2020-02-12T15:12:12Z
2021-03-19T13:46:08Z
2021-03-19T13:46:08Z