Visualizing Lignification Dynamics in Plants with Click Chemistry: Dual Labeling is BLISS!

Simon, Clemence; Spriet, Corentin; Hawkins, Simon; Lion, Cedric

Document type :

Article dans une revue scientifique

DOI :

10.3791/56947

Permalink :

http://hdl.handle.net/20.500.12210/18434

Title :

Visualizing Lignification Dynamics in Plants with Click Chemistry: Dual Labeling is BLISS!

Author(s) :

Simon, Clemence [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Spriet, Corentin [Auteur]

Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576
Hawkins, Simon [Auteur]

Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576
Lion, Cedric [Auteur]

Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576

Journal title :

Journal of visualized experiments : JoVE

Volume number :

131

Publication date :

2018-01

ISSN :

1940-087X

HAL domain(s) :

Chimie/Chimie théorique et/ou physique

English abstract : [en]

Lignin is one of the most prevalent biopolymers on the planet and a major component of lignocellulosic biomass. This phenolic polymer plays a vital structural and protective role in the development and life of higher plants. ...
Show more >Lignin is one of the most prevalent biopolymers on the planet and a major component of lignocellulosic biomass. This phenolic polymer plays a vital structural and protective role in the development and life of higher plants. Although the intricate mechanisms regulating lignification processes in vivo strongly impact the industrial valorization of many plant-derived products, the scientific community still has a long way to go to decipher them. In a simple three-step workflow, the dual labeling protocol presented herein enables bioimaging studies of actively lignifying zones of plant tissues. The first step consists in the metabolic incorporation of two independent chemical reporters, surrogates of the two native monolignols that give rise to lignin H- and G-units. After incorporation into growing lignin polymers, each reporter is then specifically labeled with its own fluorescent probe via a sequential combination of bioorthogonal SPAAC/CuAAC click reactions. Combined with lignin autofluorescence, this approach leads to the generation of three-color localization maps of lignin within plant cell walls by confocal fluorescence microscopy and provides precise spatial information on the presence or absence of active lignification machinery at the scale of plant tissues, cells and different cell wall layers.Show less >

Language :

Anglais

Audience :

Internationale

Popular science :

Non

Administrative institution(s) :

CNRS
Université de Lille

Collections :

Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576

Research team(s) :

Fibres végétales
Chemical Glycobiology

Submission date :

2020-02-12T15:12:17Z
2024-02-19T13:34:41Z

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Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 3.0 United States

Version	Link	Modification date
2	20.500.12210/18434*	2024-02-19T13:27:30Z
1	20.500.12210/18434.1	2020-02-12T15:12:17Z

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