Title :

First characterization of SmOPG1, a novel protein involved in gonad-associated processes in Schistosoma mansoni

Author(s) :

Hahnel, Steffen [Auteur]
Justus-Liebig-Universität Gießen = Justus Liebig University [JLU]
Parker-Manuel, Richard [Auteur]
Justus-Liebig-Universität Gießen = Justus Liebig University [JLU]
Dissous, Colette [Auteur]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
Cailliau, Katia [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 [UGSF]
Grevelding, Christoph G. [Auteur]
Justus-Liebig-Universität Gießen = Justus Liebig University [JLU]

Journal title :

Molecular and Biochemical Parasitology

Volume number :

213

Pages :

22-25

Publication date :

2017-04

ISSN :

0166-6851

English keyword(s) :

Schistosoma mansoni
Platyhelminth reproduction
Hypothetical protein
Mucin-like protein
Signaling
Gonad development

HAL domain(s) :

Chimie/Chimie théorique et/ou physique
Sciences du Vivant [q-bio]/Biologie cellulaire

English abstract : [en]

In the context of investigating the exceptional reproductive biology of schistosomes, several studies have focused on the identification and characterization of involved molecules. Among these are cellular tyrosine kinases ...
Show more >In the context of investigating the exceptional reproductive biology of schistosomes, several studies have focused on the identification and characterization of involved molecules. Among these are cellular tyrosine kinases (CTKs) which control differentiation processes in the female gonads. On the way to unravel CTK-mediated signaling processes in more detail, several upstream- and downstream partners of these CTKs were identified. In this context we present here first data characterizing the novel orphan gene Sm opg1. Annotated as hypothetical protein, SmOPG1 was identified as an interaction partner of the CTK SmTK6 by yeast two-hybrid library screening. Y2/3H interaction studies showed that SmTK6 binds with its SH2 domain to a specific binding motif within the C-terminus of SmOPG1. Additionally, in situ-hybridization and organ-specific RT-PCR analyses demonstrated the co-localization of SmOPG1 and SmTK6 transcripts in the gonads of adult S. mansoni. Finally, SmOPG1 knock-down provided first hints for a function in the ovary.Show less >

Language :

Anglais

Audience :

Non spécifiée

Administrative institution(s) :

CNRS
Université de Lille

Collections :

• Centre d'Infection et d'Immunité de Lille (CIIL) - U1019 - UMR 9017
• Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576

Research team(s) :

Régulation des signaux de division

Submission date :

2020-02-12T15:12:36Z
2021-03-19T14:13:36Z
2021-03-19T14:16:13Z