Evidence for P-Glycoprotein Involvement ...
Type de document :
Article dans une revue scientifique
DOI :
URL permanente :
Titre :
Evidence for P-Glycoprotein Involvement in Cell Volume Regulation Using Coulter Sizing in Flow Cytometry
Auteur(s) :
Pasquier, Jennifer [Auteur]
Weill Medical College of Cornell University [New York]
Weill Cornell Medicine [Qatar]
Rioult, Damien [Auteur]
Stress Environnementaux et BIOsurveillance des milieux aquatiques [SEBIO]
Abu-Kaoud, Nadine [Auteur]
Weill Cornell Medicine [Qatar]
Hoarau-Véchot, Jessica [Auteur]
Weill Cornell Medicine [Qatar]
Marin, Matthieu [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Le Foll, Frank [Auteur]
Laboratoire d'Ecotoxicologie - Milieux Aquatiques [LEMA]
Weill Medical College of Cornell University [New York]
Weill Cornell Medicine [Qatar]
Rioult, Damien [Auteur]
Stress Environnementaux et BIOsurveillance des milieux aquatiques [SEBIO]
Abu-Kaoud, Nadine [Auteur]
Weill Cornell Medicine [Qatar]
Hoarau-Véchot, Jessica [Auteur]
Weill Cornell Medicine [Qatar]
Marin, Matthieu [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Le Foll, Frank [Auteur]
Laboratoire d'Ecotoxicologie - Milieux Aquatiques [LEMA]
Titre de la revue :
International journal of molecular sciences
Numéro :
16
Pagination :
14318-14337
Date de publication :
2015-06-24
ISSN :
1422-0067
Mot(s)-clé(s) en anglais :
multi-drug resistance
P-glycoprotein
cell volume
flow cytometer
MCF7
P-glycoprotein
cell volume
flow cytometer
MCF7
Discipline(s) HAL :
Chimie/Chimie théorique et/ou physique
Résumé en anglais : [en]
The regulation of cell volume is an essential function that is coupled to a variety of physiological processes such as receptor recycling, excitability and contraction, cell proliferation, migration, and programmed cell ...
Lire la suite >The regulation of cell volume is an essential function that is coupled to a variety of physiological processes such as receptor recycling, excitability and contraction, cell proliferation, migration, and programmed cell death. Under stress, cells undergo emergency swelling and respond to such a phenomenon with a regulatory volume decrease (RVD) where they release cellular ions, and other osmolytes as well as a concomitant loss of water. The link between P-glycoprotein, a transmembrane transporter, and cell volume regulation is controversial, and changes in cells volume are measured using microscopy or electrophysiology. For instance, by using the patch-clamp method, our team demonstrated that chloride currents activated in the RVD were more intense and rapid in a breast cancer cell line overexpressing the P-glycoprotein (P-gp). The Cell Lab Quanta SC is a flow cytometry system that simultaneously measures electronic volume, side scatter and three fluorescent colors; altogether this provides unsurpassed population resolution and accurate cell counting. Therefore, here we propose a novel method to follow cellular volume. By using the Coulter-type channel of the cytometer Cell Lab Quanta SC MPL (multi-platform loading), we demonstrated a role for the P-gp during different osmotic treatments, but also a differential activity of the P-gp through the cell cycle. Altogether, our data strongly suggests a role of P-gp in cell volume regulation.Lire moins >
Lire la suite >The regulation of cell volume is an essential function that is coupled to a variety of physiological processes such as receptor recycling, excitability and contraction, cell proliferation, migration, and programmed cell death. Under stress, cells undergo emergency swelling and respond to such a phenomenon with a regulatory volume decrease (RVD) where they release cellular ions, and other osmolytes as well as a concomitant loss of water. The link between P-glycoprotein, a transmembrane transporter, and cell volume regulation is controversial, and changes in cells volume are measured using microscopy or electrophysiology. For instance, by using the patch-clamp method, our team demonstrated that chloride currents activated in the RVD were more intense and rapid in a breast cancer cell line overexpressing the P-glycoprotein (P-gp). The Cell Lab Quanta SC is a flow cytometry system that simultaneously measures electronic volume, side scatter and three fluorescent colors; altogether this provides unsurpassed population resolution and accurate cell counting. Therefore, here we propose a novel method to follow cellular volume. By using the Coulter-type channel of the cytometer Cell Lab Quanta SC MPL (multi-platform loading), we demonstrated a role for the P-gp during different osmotic treatments, but also a differential activity of the P-gp through the cell cycle. Altogether, our data strongly suggests a role of P-gp in cell volume regulation.Lire moins >
Langue :
Anglais
Audience :
Non spécifiée
Établissement(s) :
CNRS
Université de Lille
Université de Lille
Équipe(s) de recherche :
Régulation des signaux de division
Date de dépôt :
2020-02-12T15:44:30Z
2021-05-07T09:30:11Z
2021-05-07T09:30:11Z
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