Title :

Evidence for P-Glycoprotein Involvement in Cell Volume Regulation Using Coulter Sizing in Flow Cytometry

Author(s) :

Pasquier, Jennifer [Auteur]
Weill Cornell Medicine [Qatar]
Weill Medical College of Cornell University [New York]
Rioult, Damien [Auteur]
Stress Environnementaux et BIOsurveillance des milieux aquatiques [SEBIO]
Abu-Kaoud, Nadine [Auteur]
Weill Cornell Medicine [Qatar]
Hoarau-Véchot, Jessica [Auteur]
Weill Cornell Medicine [Qatar]
Marin, Matthieu [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 [UGSF]
Le Foll, Frank [Auteur]
Laboratoire d'Ecotoxicologie - Milieux Aquatiques [LEMA]

Journal title :

International journal of molecular sciences

Volume number :

16

Pages :

14318-14337

Publication date :

2015-06-24

ISSN :

1422-0067

English keyword(s) :

multi-drug resistance
P-glycoprotein
cell volume
flow cytometer
MCF7

HAL domain(s) :

Chimie/Chimie théorique et/ou physique

English abstract : [en]

The regulation of cell volume is an essential function that is coupled to a variety of physiological processes such as receptor recycling, excitability and contraction, cell proliferation, migration, and programmed cell ...
Show more >The regulation of cell volume is an essential function that is coupled to a variety of physiological processes such as receptor recycling, excitability and contraction, cell proliferation, migration, and programmed cell death. Under stress, cells undergo emergency swelling and respond to such a phenomenon with a regulatory volume decrease (RVD) where they release cellular ions, and other osmolytes as well as a concomitant loss of water. The link between P-glycoprotein, a transmembrane transporter, and cell volume regulation is controversial, and changes in cells volume are measured using microscopy or electrophysiology. For instance, by using the patch-clamp method, our team demonstrated that chloride currents activated in the RVD were more intense and rapid in a breast cancer cell line overexpressing the P-glycoprotein (P-gp). The Cell Lab Quanta SC is a flow cytometry system that simultaneously measures electronic volume, side scatter and three fluorescent colors; altogether this provides unsurpassed population resolution and accurate cell counting. Therefore, here we propose a novel method to follow cellular volume. By using the Coulter-type channel of the cytometer Cell Lab Quanta SC MPL (multi-platform loading), we demonstrated a role for the P-gp during different osmotic treatments, but also a differential activity of the P-gp through the cell cycle. Altogether, our data strongly suggests a role of P-gp in cell volume regulation.Show less >

Language :

Anglais

Audience :

Non spécifiée

Administrative institution(s) :

CNRS
Université de Lille

Collections :

• Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576

Research team(s) :

Régulation des signaux de division

Submission date :

2020-02-12T15:44:30Z
2021-05-07T09:30:11Z