Gonad RNA-specific qRT-PCR analyses identify ...
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Article dans une revue scientifique
DOI :
Permalink :
Title :
Gonad RNA-specific qRT-PCR analyses identify genes with potential functions in schistosome reproduction such as SmFz1 and SmFGFRs
Author(s) :
Hahnel, Steffen [Auteur]
Justus-Liebig-Universität Gießen = Justus Liebig University [JLU]
Quack, Thomas [Auteur]
Justus-Liebig-Universität Gießen = Justus Liebig University [JLU]
Parker-Manuel, Sophia J. [Auteur]
Justus-Liebig-Universität Gießen = Justus Liebig University [JLU]
Lu, Zhigang [Auteur]
Justus-Liebig-Universität Gießen = Justus Liebig University [JLU]
Vanderstraete, Mathieu [Auteur]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
Morel, Marion [Auteur]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
Dissous, Colette [Auteur]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
Cailliau, Katia [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 [UGSF]
Grevelding, Christoph G. [Auteur]
Justus-Liebig-Universität Gießen = Justus Liebig University [JLU]
Justus-Liebig-Universität Gießen = Justus Liebig University [JLU]
Quack, Thomas [Auteur]
Justus-Liebig-Universität Gießen = Justus Liebig University [JLU]
Parker-Manuel, Sophia J. [Auteur]
Justus-Liebig-Universität Gießen = Justus Liebig University [JLU]
Lu, Zhigang [Auteur]
Justus-Liebig-Universität Gießen = Justus Liebig University [JLU]
Vanderstraete, Mathieu [Auteur]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
Morel, Marion [Auteur]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
Dissous, Colette [Auteur]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
Cailliau, Katia [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 [UGSF]
Grevelding, Christoph G. [Auteur]
Justus-Liebig-Universität Gießen = Justus Liebig University [JLU]
Journal title :
Frontiers in Genetics
Volume number :
5
Publication date :
2014-06-10
ISSN :
1664-8021
English keyword(s) :
schistosomiasis
Schistosoma mansoni
development
helminth reproduction
gonad
frizzled (Fz)
fibroblast growth factor receptor (FGFR)
organ isolation
Schistosoma mansoni
development
helminth reproduction
gonad
frizzled (Fz)
fibroblast growth factor receptor (FGFR)
organ isolation
HAL domain(s) :
Chimie/Chimie théorique et/ou physique
English abstract : [en]
In the search for new strategies to fight schistosomiasis, the unique reproductive biologyofSchistosoma mansonihas come into the focus of research. The development of thegonads and the ability of egg production are fundamental ...
Show more >In the search for new strategies to fight schistosomiasis, the unique reproductive biologyofSchistosoma mansonihas come into the focus of research. The development of thegonads and the ability of egg production are fundamental not only for continuing the lifecycle but also for pathogenicity. Previous studies of schistosome biology demonstrated aninfluence of pairing on gonad development of the female and on gene expression profilesin both genders. Due to the limited access to specific tissues, however, most of thesestudies were done at the level of whole worms neglecting individual tissues that may betargets of pairing-dependent processes. Recently, we established a protocol allowing theisolation of testes and ovaries from adultS. mansoni. Here, we describe tissue-specificqRT-PCR analyses comparing transcript levels of selected genes on the basis of RNAfrom gonads and whole worms. Gene expression in ovary and testes was in some casesfound to be significantly influenced by pairing, which was not traceable in whole worms.Among the candidate genes identified as regulated by pairing in gonads were the frizzledhomolog SmFz1 and the two fibroblast growth factor receptor homologs SmFGFR-A andSmFGFR-B. First functional characterizations were done, including comparative qRT-PCRanalyses,in situ-localization experiments, heterologous expression inXenopusoocytes(SmFGFR-A/B), and inhibitor studies using the Fz/Dvl-pathway inhibitor 3289-8625, orBIBF1120 blocking FGFR-signaling. Besides confirming gonad localization and receptorfunctions, inhibitor-induced phenotypes were observedin vitrosuch as decreased eggproduction as well as drastic effects on gonad differentiation, morphology, embryogenesis,and survival of adult worms. In summary, these results emphasise the usefulnessof tissue-specific qRT-PCRs for selection of candidate genes with important roles inreproduction, allowing subsequent studies to determine their suitability as drug targets.Show less >
Show more >In the search for new strategies to fight schistosomiasis, the unique reproductive biologyofSchistosoma mansonihas come into the focus of research. The development of thegonads and the ability of egg production are fundamental not only for continuing the lifecycle but also for pathogenicity. Previous studies of schistosome biology demonstrated aninfluence of pairing on gonad development of the female and on gene expression profilesin both genders. Due to the limited access to specific tissues, however, most of thesestudies were done at the level of whole worms neglecting individual tissues that may betargets of pairing-dependent processes. Recently, we established a protocol allowing theisolation of testes and ovaries from adultS. mansoni. Here, we describe tissue-specificqRT-PCR analyses comparing transcript levels of selected genes on the basis of RNAfrom gonads and whole worms. Gene expression in ovary and testes was in some casesfound to be significantly influenced by pairing, which was not traceable in whole worms.Among the candidate genes identified as regulated by pairing in gonads were the frizzledhomolog SmFz1 and the two fibroblast growth factor receptor homologs SmFGFR-A andSmFGFR-B. First functional characterizations were done, including comparative qRT-PCRanalyses,in situ-localization experiments, heterologous expression inXenopusoocytes(SmFGFR-A/B), and inhibitor studies using the Fz/Dvl-pathway inhibitor 3289-8625, orBIBF1120 blocking FGFR-signaling. Besides confirming gonad localization and receptorfunctions, inhibitor-induced phenotypes were observedin vitrosuch as decreased eggproduction as well as drastic effects on gonad differentiation, morphology, embryogenesis,and survival of adult worms. In summary, these results emphasise the usefulnessof tissue-specific qRT-PCRs for selection of candidate genes with important roles inreproduction, allowing subsequent studies to determine their suitability as drug targets.Show less >
Language :
Anglais
Audience :
Non spécifiée
Administrative institution(s) :
CNRS
Université de Lille
Université de Lille
Research team(s) :
Régulation des signaux de division
Submission date :
2020-02-12T15:44:32Z
2021-01-08T08:34:56Z
2021-06-04T12:32:14Z
2021-01-08T08:34:56Z
2021-06-04T12:32:14Z
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