Early detection of colonic dysplasia by ...
Document type :
Article dans une revue scientifique
DOI :
Permalink :
Title :
Early detection of colonic dysplasia by magnetic resonance molecular imaging with a contrast agent raised against the colon cancer marker MUC5AC: MRI Contrast Agent Targeting MUC5AC
Author(s) :
Rossez, Yannick [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Burtea, Carmen [Auteur]
Université de Mons / University of Mons [UMONS]
Laurent, Sophie [Auteur]
Université de Mons / University of Mons [UMONS]
Gosset, Pierre [Auteur]
Université catholique de Lille [UCL]
Leonard, Renaud [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Gonzalez, Walter [Auteur]
Laboratoire Guerbet / Guerbet Research
Ballet, Sébastien [Auteur]
Laboratoire Guerbet / Guerbet Research
Raynal, Isabelle [Auteur]
Laboratoire Guerbet / Guerbet Research
Rousseaux, Olivier [Auteur]
Laboratoire Guerbet / Guerbet Research
Dugué, Timothée [Auteur]
Université catholique de Lille [UCL]
Vander Elst, Luce [Auteur]
Université de Mons / University of Mons [UMONS]
Michalski, Jean-Claude [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Muller, Robert N. [Auteur]
Université de Mons / University of Mons [UMONS]
Masselot, Catherine [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Burtea, Carmen [Auteur]
Université de Mons / University of Mons [UMONS]
Laurent, Sophie [Auteur]
Université de Mons / University of Mons [UMONS]
Gosset, Pierre [Auteur]
Université catholique de Lille [UCL]
Leonard, Renaud [Auteur]

Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Gonzalez, Walter [Auteur]
Laboratoire Guerbet / Guerbet Research
Ballet, Sébastien [Auteur]
Laboratoire Guerbet / Guerbet Research
Raynal, Isabelle [Auteur]
Laboratoire Guerbet / Guerbet Research
Rousseaux, Olivier [Auteur]
Laboratoire Guerbet / Guerbet Research
Dugué, Timothée [Auteur]
Université catholique de Lille [UCL]
Vander Elst, Luce [Auteur]
Université de Mons / University of Mons [UMONS]
Michalski, Jean-Claude [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Muller, Robert N. [Auteur]
Université de Mons / University of Mons [UMONS]
Masselot, Catherine [Auteur]

Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Journal title :
Contrast Media & Molecular Imaging
Volume number :
11
Pages :
211-221
Publication date :
2016-05
ISSN :
1555-4309
English keyword(s) :
colorectal cancer
Early Detection of Cancer
MRI
Mucin 5AC
Humans
Molecular Imaging
Peptide Library
Biomarkers, Tumor
MUC5AC
Mucin-2
Magnetic Resonance Imaging
Animals
Contrast Media
Heterografts
mucin
Sensitivity and Specificity
Cell Line, Tumor
Biomarkers
Colorectal Neoplasms
Mice
Colonic Neoplasms
Early Detection of Cancer
MRI
Mucin 5AC
Humans
Molecular Imaging
Peptide Library
Biomarkers, Tumor
MUC5AC
Mucin-2
Magnetic Resonance Imaging
Animals
Contrast Media
Heterografts
mucin
Sensitivity and Specificity
Cell Line, Tumor
Biomarkers
Colorectal Neoplasms
Mice
Colonic Neoplasms
HAL domain(s) :
Chimie/Chimie théorique et/ou physique
English abstract : [en]
Human gastric mucin MUC5AC is secreted in the colonic mucus of cancer patients and is a specific marker of precancerous lesions called aberrant crypt foci. Using MUC5AC as a specific marker can improve sensitivity in the ...
Show more >Human gastric mucin MUC5AC is secreted in the colonic mucus of cancer patients and is a specific marker of precancerous lesions called aberrant crypt foci. Using MUC5AC as a specific marker can improve sensitivity in the detection of early colorectal cancer. Here we demonstrated that the accumulation of MUC5AC in xenograft and mouse stomach can be detected by magnetic resonance imaging (MRI). We used ultrasmall particles of iron oxide (USPIOs) conjugated with disulfide constrained heptapeptide that were identified using a screening phage display. To accomplish this, we employed positive selection of the phage display library on MUC5AC purified from fresh human colonic adenomas in combination with negative selection of the phage library on purified human MUC2, which is predominantly found in normal colorectal tissues. This conjugate was tested on human colorectal cancer cell lines that were either able or unable to secrete MUC5AC, both in vitro and in vivo. MUC5AC–USPIO contrast agent and USPIOs alone were not detected in cell lines unable to secrete MUC5AC. A combination of MRI and microscopy studies was performed to detect a specific accumulation of the contrast agent in vivo. Thus, the MUC5AC contrast agent enabled non‐invasive detection of precancerous lesions and colorectal cancer, highlighting its potential use in diagnostics, in the early detection of colorectal cancer recurrences after treatment and in mechanistic studies implicating MUC5AC.Show less >
Show more >Human gastric mucin MUC5AC is secreted in the colonic mucus of cancer patients and is a specific marker of precancerous lesions called aberrant crypt foci. Using MUC5AC as a specific marker can improve sensitivity in the detection of early colorectal cancer. Here we demonstrated that the accumulation of MUC5AC in xenograft and mouse stomach can be detected by magnetic resonance imaging (MRI). We used ultrasmall particles of iron oxide (USPIOs) conjugated with disulfide constrained heptapeptide that were identified using a screening phage display. To accomplish this, we employed positive selection of the phage display library on MUC5AC purified from fresh human colonic adenomas in combination with negative selection of the phage library on purified human MUC2, which is predominantly found in normal colorectal tissues. This conjugate was tested on human colorectal cancer cell lines that were either able or unable to secrete MUC5AC, both in vitro and in vivo. MUC5AC–USPIO contrast agent and USPIOs alone were not detected in cell lines unable to secrete MUC5AC. A combination of MRI and microscopy studies was performed to detect a specific accumulation of the contrast agent in vivo. Thus, the MUC5AC contrast agent enabled non‐invasive detection of precancerous lesions and colorectal cancer, highlighting its potential use in diagnostics, in the early detection of colorectal cancer recurrences after treatment and in mechanistic studies implicating MUC5AC.Show less >
Language :
Anglais
Audience :
Non spécifiée
Administrative institution(s) :
CNRS
Université de Lille
Université de Lille
Research team(s) :
Mécanismes moléculaires de la N-glycosylation et pathologies associées
Génétique des enveloppes bactériennes
Génétique des enveloppes bactériennes
Submission date :
2020-02-12T15:44:37Z
2021-04-22T06:56:50Z
2021-04-22T06:56:50Z