The two‐component system CpxAR is essential ...
Document type :
Article dans une revue scientifique
DOI :
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Title :
The two‐component system CpxAR is essential for virulence in the phytopathogen bacteria Dickeya dadantii EC3937
Author(s) :
bontemps-gallo, sébastien [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Madec, Edwige [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Lacroix, Jean-Marie [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]

Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Madec, Edwige [Auteur]

Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Lacroix, Jean-Marie [Auteur]

Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Journal title :
Environmental Microbiology
Volume number :
17
Pages :
4415-4428
Publication date :
2015-11
ISSN :
1462-2912
English keyword(s) :
CpxAR
Virulence
stress
plant pathogen
D. dadantii
Virulence
stress
plant pathogen
D. dadantii
HAL domain(s) :
Chimie/Chimie théorique et/ou physique
English abstract : [en]
The CpxAR two‐component system is present in many Proteobacteria. It controls expression of genes required to maintain envelope integrity in response to environmental injury. Consequently, this two‐component system was ...
Show more >The CpxAR two‐component system is present in many Proteobacteria. It controls expression of genes required to maintain envelope integrity in response to environmental injury. Consequently, this two‐component system was shown to be required for virulence of several zoo‐pathogens, but it has never been investigated in phyto‐pathogens. In this paper, we investigate the role of the CpxAR two‐component system in vitro and in vivo in Dickeya dadantii, an enterobacterial phytopathogen that causes soft‐rot disease in a large variety of plant species. cpxA null mutant displays a constitutively phosphorylated CpxR phenotype as shown by direct analysis of phosphorylation of CpxR by a Phos‐Tag retardation gel approach. Virulence in plants is completely abolished in cpxA or cpxR mutants of D. dadantii. In planta, CpxAR is only activated at an early stage of the infection process as shown by Phos‐Tag and gene fusion analyses. To our knowledge, this is the first time that the timing of CpxAR phosphorelay activation has been investigated during the infection process by direct monitoring of response regulator phosphorylation.Show less >
Show more >The CpxAR two‐component system is present in many Proteobacteria. It controls expression of genes required to maintain envelope integrity in response to environmental injury. Consequently, this two‐component system was shown to be required for virulence of several zoo‐pathogens, but it has never been investigated in phyto‐pathogens. In this paper, we investigate the role of the CpxAR two‐component system in vitro and in vivo in Dickeya dadantii, an enterobacterial phytopathogen that causes soft‐rot disease in a large variety of plant species. cpxA null mutant displays a constitutively phosphorylated CpxR phenotype as shown by direct analysis of phosphorylation of CpxR by a Phos‐Tag retardation gel approach. Virulence in plants is completely abolished in cpxA or cpxR mutants of D. dadantii. In planta, CpxAR is only activated at an early stage of the infection process as shown by Phos‐Tag and gene fusion analyses. To our knowledge, this is the first time that the timing of CpxAR phosphorelay activation has been investigated during the infection process by direct monitoring of response regulator phosphorylation.Show less >
Language :
Anglais
Audience :
Non spécifiée
Administrative institution(s) :
CNRS
Université de Lille
Université de Lille
Research team(s) :
Génétique des enveloppes bactériennes
Submission date :
2020-02-12T15:44:38Z
2021-04-22T11:30:21Z
2021-04-22T11:30:21Z
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