The two‐component system CpxAR is essential ...
Type de document :
Article dans une revue scientifique
DOI :
URL permanente :
Titre :
The two‐component system CpxAR is essential for virulence in the phytopathogen bacteria Dickeya dadantii EC3937
Auteur(s) :
bontemps-gallo, sébastien [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Madec, Edwige [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 [UGSF]
Lacroix, Jean-Marie [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 [UGSF]
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Madec, Edwige [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 [UGSF]
Lacroix, Jean-Marie [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 [UGSF]
Titre de la revue :
Environmental Microbiology
Numéro :
17
Pagination :
4415-4428
Date de publication :
2015-11
ISSN :
1462-2912
Mot(s)-clé(s) en anglais :
CpxAR
Virulence
stress
plant pathogen
D. dadantii
Virulence
stress
plant pathogen
D. dadantii
Discipline(s) HAL :
Chimie/Chimie théorique et/ou physique
Résumé en anglais : [en]
The CpxAR two‐component system is present in many Proteobacteria. It controls expression of genes required to maintain envelope integrity in response to environmental injury. Consequently, this two‐component system was ...
Lire la suite >The CpxAR two‐component system is present in many Proteobacteria. It controls expression of genes required to maintain envelope integrity in response to environmental injury. Consequently, this two‐component system was shown to be required for virulence of several zoo‐pathogens, but it has never been investigated in phyto‐pathogens. In this paper, we investigate the role of the CpxAR two‐component system in vitro and in vivo in Dickeya dadantii, an enterobacterial phytopathogen that causes soft‐rot disease in a large variety of plant species. cpxA null mutant displays a constitutively phosphorylated CpxR phenotype as shown by direct analysis of phosphorylation of CpxR by a Phos‐Tag retardation gel approach. Virulence in plants is completely abolished in cpxA or cpxR mutants of D. dadantii. In planta, CpxAR is only activated at an early stage of the infection process as shown by Phos‐Tag and gene fusion analyses. To our knowledge, this is the first time that the timing of CpxAR phosphorelay activation has been investigated during the infection process by direct monitoring of response regulator phosphorylation.Lire moins >
Lire la suite >The CpxAR two‐component system is present in many Proteobacteria. It controls expression of genes required to maintain envelope integrity in response to environmental injury. Consequently, this two‐component system was shown to be required for virulence of several zoo‐pathogens, but it has never been investigated in phyto‐pathogens. In this paper, we investigate the role of the CpxAR two‐component system in vitro and in vivo in Dickeya dadantii, an enterobacterial phytopathogen that causes soft‐rot disease in a large variety of plant species. cpxA null mutant displays a constitutively phosphorylated CpxR phenotype as shown by direct analysis of phosphorylation of CpxR by a Phos‐Tag retardation gel approach. Virulence in plants is completely abolished in cpxA or cpxR mutants of D. dadantii. In planta, CpxAR is only activated at an early stage of the infection process as shown by Phos‐Tag and gene fusion analyses. To our knowledge, this is the first time that the timing of CpxAR phosphorelay activation has been investigated during the infection process by direct monitoring of response regulator phosphorylation.Lire moins >
Langue :
Anglais
Audience :
Non spécifiée
Établissement(s) :
CNRS
Université de Lille
Université de Lille
Équipe(s) de recherche :
Génétique des enveloppes bactériennes
Date de dépôt :
2020-02-12T15:44:38Z
2021-04-22T11:30:21Z
2021-04-22T11:30:21Z
Fichiers
- documen
- Accès libre
- Accéder au document